Electrochemical Investigation of Curcumin–DNA Interaction by Using Hydroxyapatite Nanoparticles–Ionic Liquids Based Composite Electrodes
Hydroxyapatite nanoparticles (HaP) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) are newly developed in this assay. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and cyclic voltammetry (CV) were applie...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/14/15/4344 |
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| author | Merve Uca Ece Eksin Yasemin Erac Arzum Erdem |
| author_facet | Merve Uca Ece Eksin Yasemin Erac Arzum Erdem |
| author_sort | Merve Uca |
| collection | DOAJ |
| description | Hydroxyapatite nanoparticles (HaP) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) are newly developed in this assay. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and cyclic voltammetry (CV) were applied to examine the microscopic and electrochemical characterization of HaP and IL-modified biosensors. The interaction of curcumin with nucleic acids and polymerase chain reaction (PCR) samples was investigated by measuring the changes at the oxidation signals of both curcumin and guanine by differential pulse voltammetry (DPV) technique. The optimization of curcumin concentration, DNA concentration, and the interaction time was performed. The interaction of curcumin with PCR samples was also investigated by gel electrophoresis. |
| first_indexed | 2024-03-10T09:12:02Z |
| format | Article |
| id | doaj.art-6a455452ae6044d790d4df9771436316 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T09:12:02Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-6a455452ae6044d790d4df97714363162023-11-22T05:55:23ZengMDPI AGMaterials1996-19442021-08-011415434410.3390/ma14154344Electrochemical Investigation of Curcumin–DNA Interaction by Using Hydroxyapatite Nanoparticles–Ionic Liquids Based Composite ElectrodesMerve Uca0Ece Eksin1Yasemin Erac2Arzum Erdem3Biotechnology Department, Graduate School of Natural and Applied Sciences, Ege University, 35100 Izmir, TurkeyAnalytical Chemistry Department, Faculty of Pharmacy, Ege University, 35100 Izmir, TurkeyPharmacology Department, Faculty of Pharmacy, Ege University, 35100 Izmir, TurkeyBiotechnology Department, Graduate School of Natural and Applied Sciences, Ege University, 35100 Izmir, TurkeyHydroxyapatite nanoparticles (HaP) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) are newly developed in this assay. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and cyclic voltammetry (CV) were applied to examine the microscopic and electrochemical characterization of HaP and IL-modified biosensors. The interaction of curcumin with nucleic acids and polymerase chain reaction (PCR) samples was investigated by measuring the changes at the oxidation signals of both curcumin and guanine by differential pulse voltammetry (DPV) technique. The optimization of curcumin concentration, DNA concentration, and the interaction time was performed. The interaction of curcumin with PCR samples was also investigated by gel electrophoresis.https://www.mdpi.com/1996-1944/14/15/4344hydroxyapatite nanoparticlesionic liquidbionanomaterialsnucleic acid interactionscurcuminelectrochemical biosensors |
| spellingShingle | Merve Uca Ece Eksin Yasemin Erac Arzum Erdem Electrochemical Investigation of Curcumin–DNA Interaction by Using Hydroxyapatite Nanoparticles–Ionic Liquids Based Composite Electrodes Materials hydroxyapatite nanoparticles ionic liquid bionanomaterials nucleic acid interactions curcumin electrochemical biosensors |
| title | Electrochemical Investigation of Curcumin–DNA Interaction by Using Hydroxyapatite Nanoparticles–Ionic Liquids Based Composite Electrodes |
| title_full | Electrochemical Investigation of Curcumin–DNA Interaction by Using Hydroxyapatite Nanoparticles–Ionic Liquids Based Composite Electrodes |
| title_fullStr | Electrochemical Investigation of Curcumin–DNA Interaction by Using Hydroxyapatite Nanoparticles–Ionic Liquids Based Composite Electrodes |
| title_full_unstemmed | Electrochemical Investigation of Curcumin–DNA Interaction by Using Hydroxyapatite Nanoparticles–Ionic Liquids Based Composite Electrodes |
| title_short | Electrochemical Investigation of Curcumin–DNA Interaction by Using Hydroxyapatite Nanoparticles–Ionic Liquids Based Composite Electrodes |
| title_sort | electrochemical investigation of curcumin dna interaction by using hydroxyapatite nanoparticles ionic liquids based composite electrodes |
| topic | hydroxyapatite nanoparticles ionic liquid bionanomaterials nucleic acid interactions curcumin electrochemical biosensors |
| url | https://www.mdpi.com/1996-1944/14/15/4344 |
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