The Effect of Sialic Acid on Viability and Growth of Mice Astrocytes and Human Astroglia Cells
Background: The surface of all vertebrate cells is covered with sugar chains like sialic acid that are found at high levels in sialoglycan form in vertebrate brain. There are growing evidences that changes in level of sialic acid may cause neurodegenerative and psychotic disorders. Duo to increasing...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Isfahan University of Medical Sciences
2018-05-01
|
| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/9566 |
| _version_ | 1797720271800500224 |
|---|---|
| author | Maryam Cheraghzadeh Shirin Azizidoost Zahra Nazeri Sadegh Saremi Hamid Galehdari Alireza Kheirollah |
| author_facet | Maryam Cheraghzadeh Shirin Azizidoost Zahra Nazeri Sadegh Saremi Hamid Galehdari Alireza Kheirollah |
| author_sort | Maryam Cheraghzadeh |
| collection | DOAJ |
| description | Background: The surface of all vertebrate cells is covered with sugar chains like sialic acid that are found at high levels in sialoglycan form in vertebrate brain. There are growing evidences that changes in level of sialic acid may cause neurodegenerative and psychotic disorders. Duo to increasing growth of these diseases, and the important role of sialic acid in causing and progression of neurogenic disease, determination of toxic levels of sialic acid is important to design the in-vitro and in-vivo experiments. So, the goal of this research was evaluation of the proper concentration of sialic acid for brain cell lines.
Methods: The human astroglia cells and mice astrocytes were treated with a serial dilution of sialic acid in 96 well plates. Toxic level of sialic acid was examined using MTT assay [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide or thiazolyl blue].
Findings: The half maximal inhibitory concentration (IC50) for astroglia and astrocyte cells were 1273.3 and 3750 µM, respectively.
Conclusion: Sialic acid has a dose-dependent inhibitory effect on brain cell growth with a significant growth limitation at concentrations higher than the IC50. In addition, the results showed significant differences between the two human and mouse cells, and it was suggested that instead of the mouse model, human brain cells should be used to obtain generalized results for humans. |
| first_indexed | 2024-03-12T09:16:59Z |
| format | Article |
| id | doaj.art-6a455ac91dbf4ba4b564ddf2cf0c1655 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:16:59Z |
| publishDate | 2018-05-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-6a455ac91dbf4ba4b564ddf2cf0c16552023-09-02T14:45:42ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2018-05-013647226426910.22122/jims.v36i472.95662939The Effect of Sialic Acid on Viability and Growth of Mice Astrocytes and Human Astroglia CellsMaryam Cheraghzadeh0Shirin Azizidoost1Zahra Nazeri2Sadegh Saremi3Hamid Galehdari4Alireza Kheirollah5PhD in Molecular Genetics, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranPhD Student, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranMSc Student, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranProfessor, Department of Genetics, School of Science, Shahid Chamran University of Ahvaz, Ahvaz, IranAssociate Professor, Department of Biochemistry, School of Medicine AND Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranBackground: The surface of all vertebrate cells is covered with sugar chains like sialic acid that are found at high levels in sialoglycan form in vertebrate brain. There are growing evidences that changes in level of sialic acid may cause neurodegenerative and psychotic disorders. Duo to increasing growth of these diseases, and the important role of sialic acid in causing and progression of neurogenic disease, determination of toxic levels of sialic acid is important to design the in-vitro and in-vivo experiments. So, the goal of this research was evaluation of the proper concentration of sialic acid for brain cell lines. Methods: The human astroglia cells and mice astrocytes were treated with a serial dilution of sialic acid in 96 well plates. Toxic level of sialic acid was examined using MTT assay [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide or thiazolyl blue]. Findings: The half maximal inhibitory concentration (IC50) for astroglia and astrocyte cells were 1273.3 and 3750 µM, respectively. Conclusion: Sialic acid has a dose-dependent inhibitory effect on brain cell growth with a significant growth limitation at concentrations higher than the IC50. In addition, the results showed significant differences between the two human and mouse cells, and it was suggested that instead of the mouse model, human brain cells should be used to obtain generalized results for humans.http://jims.mui.ac.ir/index.php/jims/article/view/9566Cell cultureSialic acidAstrogliaAstrocytesThiazolyl blue |
| spellingShingle | Maryam Cheraghzadeh Shirin Azizidoost Zahra Nazeri Sadegh Saremi Hamid Galehdari Alireza Kheirollah The Effect of Sialic Acid on Viability and Growth of Mice Astrocytes and Human Astroglia Cells مجله دانشکده پزشکی اصفهان Cell culture Sialic acid Astroglia Astrocytes Thiazolyl blue |
| title | The Effect of Sialic Acid on Viability and Growth of Mice Astrocytes and Human Astroglia Cells |
| title_full | The Effect of Sialic Acid on Viability and Growth of Mice Astrocytes and Human Astroglia Cells |
| title_fullStr | The Effect of Sialic Acid on Viability and Growth of Mice Astrocytes and Human Astroglia Cells |
| title_full_unstemmed | The Effect of Sialic Acid on Viability and Growth of Mice Astrocytes and Human Astroglia Cells |
| title_short | The Effect of Sialic Acid on Viability and Growth of Mice Astrocytes and Human Astroglia Cells |
| title_sort | effect of sialic acid on viability and growth of mice astrocytes and human astroglia cells |
| topic | Cell culture Sialic acid Astroglia Astrocytes Thiazolyl blue |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/9566 |
| work_keys_str_mv | AT maryamcheraghzadeh theeffectofsialicacidonviabilityandgrowthofmiceastrocytesandhumanastrogliacells AT shirinazizidoost theeffectofsialicacidonviabilityandgrowthofmiceastrocytesandhumanastrogliacells AT zahranazeri theeffectofsialicacidonviabilityandgrowthofmiceastrocytesandhumanastrogliacells AT sadeghsaremi theeffectofsialicacidonviabilityandgrowthofmiceastrocytesandhumanastrogliacells AT hamidgalehdari theeffectofsialicacidonviabilityandgrowthofmiceastrocytesandhumanastrogliacells AT alirezakheirollah theeffectofsialicacidonviabilityandgrowthofmiceastrocytesandhumanastrogliacells AT maryamcheraghzadeh effectofsialicacidonviabilityandgrowthofmiceastrocytesandhumanastrogliacells AT shirinazizidoost effectofsialicacidonviabilityandgrowthofmiceastrocytesandhumanastrogliacells AT zahranazeri effectofsialicacidonviabilityandgrowthofmiceastrocytesandhumanastrogliacells AT sadeghsaremi effectofsialicacidonviabilityandgrowthofmiceastrocytesandhumanastrogliacells AT hamidgalehdari effectofsialicacidonviabilityandgrowthofmiceastrocytesandhumanastrogliacells AT alirezakheirollah effectofsialicacidonviabilityandgrowthofmiceastrocytesandhumanastrogliacells |