New sequence type in multidrug-resistant Klebsiella pneumoniae harboring the blaNDM-1-encoding gene in Brazil

Objectives: The aim of this study was to investigate the blaNDM gene, pathogenic potential, and antimicrobial resistance of clinical isolates of carbapenem-resistant Klebsiella pneumoniae isolated from patients admitted to the University Hospital of Londrina between January 2014 and March 2017. Meth...

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Main Authors: Roseane Galdioli Nava, Mariana Oliveira-Silva, Rafael Nakamura-Silva, André Pitondo-Silva, Eliana Carolina Vespero
Format: Article
Language:English
Published: Elsevier 2019-02-01
Series:International Journal of Infectious Diseases
Online Access:http://www.sciencedirect.com/science/article/pii/S1201971218345910
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author Roseane Galdioli Nava
Mariana Oliveira-Silva
Rafael Nakamura-Silva
André Pitondo-Silva
Eliana Carolina Vespero
author_facet Roseane Galdioli Nava
Mariana Oliveira-Silva
Rafael Nakamura-Silva
André Pitondo-Silva
Eliana Carolina Vespero
author_sort Roseane Galdioli Nava
collection DOAJ
description Objectives: The aim of this study was to investigate the blaNDM gene, pathogenic potential, and antimicrobial resistance of clinical isolates of carbapenem-resistant Klebsiella pneumoniae isolated from patients admitted to the University Hospital of Londrina between January 2014 and March 2017. Methods: blaNDM-1 and virulence genes were investigated using conventional PCR methods Antimicrobial susceptibility testing was performed by disk diffusion method according to the Clinical and Laboratory Standards Institute guidelines of 2017. Clonal relationships of the New Delhi metallo-β-lactamase (NDM)-positive isolates were determined by enterobacterial repetitive intergenic consensus (ERIC)-PCR and multilocus sequence typing (MLST). Results: A total of 825 K. pneumoniae were identified, with four isolates (Kp6408, Kp6410, Kp6411, and Kp6715) presenting the blaNDM-1 gene. All NDM-1-producing isolates showed co-production of blaKPC-2 and blaTEM genes and also the virulence genes kfu, entB, mrkD, and fimH. Three isolates (Kp6408, Kp6410, and Kp6715) were classified as multidrug-resistant (MDR) and one (Kp6411) as extensively drug-resistant (XDR). ERIC-PCR analyses demonstrated that the isolates shared about 60% genetic similarity. MLST revealed four different sequence types (STs), described for the first time in Brazil, with two novel STs described in this study: ST3371 and ST3372. Conclusion: This study reports the identification of NDM-1 associated with KPC and virulence genes in four MDR K. pneumoniae with STs first described in Brazil. Keywords: MLST, Carbapenemases, Klebsiella pneumoniae, NDM
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spelling doaj.art-6a47e904daf84ff4ac6328560c7aa3022022-12-22T00:03:28ZengElsevierInternational Journal of Infectious Diseases1201-97122019-02-0179101103New sequence type in multidrug-resistant Klebsiella pneumoniae harboring the blaNDM-1-encoding gene in BrazilRoseane Galdioli Nava0Mariana Oliveira-Silva1Rafael Nakamura-Silva2André Pitondo-Silva3Eliana Carolina Vespero4Department of Pathology, Clinical and Toxicological Analysis, State University of Londrina, Londrina, Paraná, BrazilSchool of Dentistry, University of Ribeirão Preto, Ribeirão Preto, São Paulo, BrazilSchool of Dentistry, University of Ribeirão Preto, Ribeirão Preto, São Paulo, BrazilSchool of Dentistry, University of Ribeirão Preto, Ribeirão Preto, São Paulo, BrazilDepartment of Pathology, Clinical and Toxicological Analysis, State University of Londrina, Londrina, Paraná, Brazil; Corresponding author.Objectives: The aim of this study was to investigate the blaNDM gene, pathogenic potential, and antimicrobial resistance of clinical isolates of carbapenem-resistant Klebsiella pneumoniae isolated from patients admitted to the University Hospital of Londrina between January 2014 and March 2017. Methods: blaNDM-1 and virulence genes were investigated using conventional PCR methods Antimicrobial susceptibility testing was performed by disk diffusion method according to the Clinical and Laboratory Standards Institute guidelines of 2017. Clonal relationships of the New Delhi metallo-β-lactamase (NDM)-positive isolates were determined by enterobacterial repetitive intergenic consensus (ERIC)-PCR and multilocus sequence typing (MLST). Results: A total of 825 K. pneumoniae were identified, with four isolates (Kp6408, Kp6410, Kp6411, and Kp6715) presenting the blaNDM-1 gene. All NDM-1-producing isolates showed co-production of blaKPC-2 and blaTEM genes and also the virulence genes kfu, entB, mrkD, and fimH. Three isolates (Kp6408, Kp6410, and Kp6715) were classified as multidrug-resistant (MDR) and one (Kp6411) as extensively drug-resistant (XDR). ERIC-PCR analyses demonstrated that the isolates shared about 60% genetic similarity. MLST revealed four different sequence types (STs), described for the first time in Brazil, with two novel STs described in this study: ST3371 and ST3372. Conclusion: This study reports the identification of NDM-1 associated with KPC and virulence genes in four MDR K. pneumoniae with STs first described in Brazil. Keywords: MLST, Carbapenemases, Klebsiella pneumoniae, NDMhttp://www.sciencedirect.com/science/article/pii/S1201971218345910
spellingShingle Roseane Galdioli Nava
Mariana Oliveira-Silva
Rafael Nakamura-Silva
André Pitondo-Silva
Eliana Carolina Vespero
New sequence type in multidrug-resistant Klebsiella pneumoniae harboring the blaNDM-1-encoding gene in Brazil
International Journal of Infectious Diseases
title New sequence type in multidrug-resistant Klebsiella pneumoniae harboring the blaNDM-1-encoding gene in Brazil
title_full New sequence type in multidrug-resistant Klebsiella pneumoniae harboring the blaNDM-1-encoding gene in Brazil
title_fullStr New sequence type in multidrug-resistant Klebsiella pneumoniae harboring the blaNDM-1-encoding gene in Brazil
title_full_unstemmed New sequence type in multidrug-resistant Klebsiella pneumoniae harboring the blaNDM-1-encoding gene in Brazil
title_short New sequence type in multidrug-resistant Klebsiella pneumoniae harboring the blaNDM-1-encoding gene in Brazil
title_sort new sequence type in multidrug resistant klebsiella pneumoniae harboring the blandm 1 encoding gene in brazil
url http://www.sciencedirect.com/science/article/pii/S1201971218345910
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