Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue
<p>Abstract</p> <p>Background</p> <p>As Chronic Fatigue Syndrome (CFS) has been known to follow Epstein-Bar virus (EBV) and other systemic infections; our objective was to describe differences in immune activation in post-infective CFS (PI-CFS) patients and recovered co...
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Format: | Article |
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BMC
2012-09-01
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Series: | Journal of Translational Medicine |
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Online Access: | http://www.translational-medicine.com/content/10/1/191 |
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author | Broderick Gordon Katz Ben Z Fernandes Henrique Fletcher Mary Klimas Nancy Smith Frederick A O’Gorman Maurice RG Vernon Suzanne D Taylor Renee |
author_facet | Broderick Gordon Katz Ben Z Fernandes Henrique Fletcher Mary Klimas Nancy Smith Frederick A O’Gorman Maurice RG Vernon Suzanne D Taylor Renee |
author_sort | Broderick Gordon |
collection | DOAJ |
description | <p>Abstract</p> <p>Background</p> <p>As Chronic Fatigue Syndrome (CFS) has been known to follow Epstein-Bar virus (EBV) and other systemic infections; our objective was to describe differences in immune activation in post-infective CFS (PI-CFS) patients and recovered controls. We studied 301 adolescents prospectively over 24 months following the diagnosis of monospot-positive infectious mononucleosis (IM). We found an incidence of CFS at 6, 12 and 24 months of 13%, 7% and 4% respectively.</p> <p>Methods</p> <p>Using chemiluminescent imaging we measured the concentrations of IL-1a, 1b, 2, 4, 5, 6, 8, 10, 12 (p70), 13, 15, 17 and 23, IFN-γ, TNF-α and TNF-β in duplicate plasma samples available in bio-bank from 9 PI-CFS subjects and 12 recovered controls at 24 months post-infection.</p> <p>Results</p> <p>Standard comparative analysis indicated significant differences in IL-8 and 23 across subject groups. In constructing a linear classification model IL-6, 8 and 23 were selected by two different statistical approaches as discriminating features, with IL-1a, IL-2 and IFN-γ also selected in one model or the other. This supported an assignment accuracy of better than 80% at a confidence level of 0.95 into PI-CFS versus recovered controls.</p> <p>Conclusion</p> <p>These results suggest that co-expression patterns in as few as 5 cytokines associated with Th17 function may hold promise as a tool for the diagnosis of post-infectious CFS.</p> |
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id | doaj.art-6a48fed5b9d04273a8cc5bf94699d154 |
institution | Directory Open Access Journal |
issn | 1479-5876 |
language | English |
last_indexed | 2024-04-13T11:09:51Z |
publishDate | 2012-09-01 |
publisher | BMC |
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series | Journal of Translational Medicine |
spelling | doaj.art-6a48fed5b9d04273a8cc5bf94699d1542022-12-22T02:49:09ZengBMCJournal of Translational Medicine1479-58762012-09-0110119110.1186/1479-5876-10-191Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigueBroderick GordonKatz Ben ZFernandes HenriqueFletcher MaryKlimas NancySmith Frederick AO’Gorman Maurice RGVernon Suzanne DTaylor Renee<p>Abstract</p> <p>Background</p> <p>As Chronic Fatigue Syndrome (CFS) has been known to follow Epstein-Bar virus (EBV) and other systemic infections; our objective was to describe differences in immune activation in post-infective CFS (PI-CFS) patients and recovered controls. We studied 301 adolescents prospectively over 24 months following the diagnosis of monospot-positive infectious mononucleosis (IM). We found an incidence of CFS at 6, 12 and 24 months of 13%, 7% and 4% respectively.</p> <p>Methods</p> <p>Using chemiluminescent imaging we measured the concentrations of IL-1a, 1b, 2, 4, 5, 6, 8, 10, 12 (p70), 13, 15, 17 and 23, IFN-γ, TNF-α and TNF-β in duplicate plasma samples available in bio-bank from 9 PI-CFS subjects and 12 recovered controls at 24 months post-infection.</p> <p>Results</p> <p>Standard comparative analysis indicated significant differences in IL-8 and 23 across subject groups. In constructing a linear classification model IL-6, 8 and 23 were selected by two different statistical approaches as discriminating features, with IL-1a, IL-2 and IFN-γ also selected in one model or the other. This supported an assignment accuracy of better than 80% at a confidence level of 0.95 into PI-CFS versus recovered controls.</p> <p>Conclusion</p> <p>These results suggest that co-expression patterns in as few as 5 cytokines associated with Th17 function may hold promise as a tool for the diagnosis of post-infectious CFS.</p>http://www.translational-medicine.com/content/10/1/191CytokinesInfectious mononucleosisChronic fatigueClassification model |
spellingShingle | Broderick Gordon Katz Ben Z Fernandes Henrique Fletcher Mary Klimas Nancy Smith Frederick A O’Gorman Maurice RG Vernon Suzanne D Taylor Renee Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue Journal of Translational Medicine Cytokines Infectious mononucleosis Chronic fatigue Classification model |
title | Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue |
title_full | Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue |
title_fullStr | Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue |
title_full_unstemmed | Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue |
title_short | Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue |
title_sort | cytokine expression profiles of immune imbalance in post mononucleosis chronic fatigue |
topic | Cytokines Infectious mononucleosis Chronic fatigue Classification model |
url | http://www.translational-medicine.com/content/10/1/191 |
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