Expression of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significance
Objective: To investigate the expression level of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significance. Methods: SP immunohistochemical and Western blot analysis were used to detect the expression of P53, PTEN and S100A4 protein in invasive ductal breast cancer, adj...
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| Format: | Article |
| Language: | English |
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Manipal College of Medical Sciences, Pokhara
2015-05-01
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| Series: | Asian Journal of Medical Sciences |
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| Online Access: | https://www.nepjol.info/index.php/AJMS/article/view/12599 |
| _version_ | 1819296372997750784 |
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| author | Zhang Gang |
| author_facet | Zhang Gang |
| author_sort | Zhang Gang |
| collection | DOAJ |
| description | Objective: To investigate the expression level of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significance.
Methods: SP immunohistochemical and Western blot analysis were used to detect the expression of P53, PTEN and S100A4 protein in invasive ductal breast cancer, adjacent tissues and benign breast lesions. The results were aimed to explore the correlation of p53, PTEN, S100A4 and analyze the relationship between indicators and clinical pathology.
Results: Immunohistochemical results showed that the positive rates of p53 and S100A4 in invasive ductal breast cancer were 47.69% and 56.92%, which were significantly higher than that in benign breast lesions (16.00%, 22.00%) (p<0.05); The positive rates of PTEN in invasive ductal breast cancer was 24.62%, which were significantly lower than that in benign breast lesions (52.00%) (p<0.05); The expression of p53 was negatively correlated with PTEN in invasive ductal breast cancer (p<0.05). Western blot results revealed that the relative expression levels of p53, PTEN and S100A4 protein in invasive ductal breast cancer were 0.967±0.066, 0.785±0.044, 1.065±0.073, which were significant difference with that in benign breast lesions (0.549±0.032, 1.245±0.059, 0.370±0.065) (p<0.05); The expression of p53 has correlation with histological differentiating degree and lymph node metastasis (p<0.05), but it was irrelevant to the age, tumor size and clinical grade (p>0.05). The expression of S100A4 has correlation with clinical grade and lymph node metastasis (p<0.05), but no association with the age, tumor size and histological differentiating degree was observed in this study (p>0.05). The expressions of PTEN has correlation with lymph node metastasis p<0.05, but no association with the age, tumor size, clinical grade and histological differentiating degree was noted in our study (p>0.05).
Conclusion: Expression of P53, PTEN and S100A4 can be expected to be taken as to the prognosis biomarkers of breast cancer and potential targets for new and effective treatment strategies for breast cancer. |
| first_indexed | 2024-12-24T04:57:04Z |
| format | Article |
| id | doaj.art-6a492478e77643e88ffa68dd129a5d1a |
| institution | Directory Open Access Journal |
| issn | 2467-9100 2091-0576 |
| language | English |
| last_indexed | 2024-12-24T04:57:04Z |
| publishDate | 2015-05-01 |
| publisher | Manipal College of Medical Sciences, Pokhara |
| record_format | Article |
| series | Asian Journal of Medical Sciences |
| spelling | doaj.art-6a492478e77643e88ffa68dd129a5d1a2022-12-21T17:14:22ZengManipal College of Medical Sciences, PokharaAsian Journal of Medical Sciences2467-91002091-05762015-05-01664347https://doi.org/10.3126/ajms.v6i6.12599Expression of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significanceZhang Gang 0Hospital Of Hebei University,Objective: To investigate the expression level of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significance. Methods: SP immunohistochemical and Western blot analysis were used to detect the expression of P53, PTEN and S100A4 protein in invasive ductal breast cancer, adjacent tissues and benign breast lesions. The results were aimed to explore the correlation of p53, PTEN, S100A4 and analyze the relationship between indicators and clinical pathology. Results: Immunohistochemical results showed that the positive rates of p53 and S100A4 in invasive ductal breast cancer were 47.69% and 56.92%, which were significantly higher than that in benign breast lesions (16.00%, 22.00%) (p<0.05); The positive rates of PTEN in invasive ductal breast cancer was 24.62%, which were significantly lower than that in benign breast lesions (52.00%) (p<0.05); The expression of p53 was negatively correlated with PTEN in invasive ductal breast cancer (p<0.05). Western blot results revealed that the relative expression levels of p53, PTEN and S100A4 protein in invasive ductal breast cancer were 0.967±0.066, 0.785±0.044, 1.065±0.073, which were significant difference with that in benign breast lesions (0.549±0.032, 1.245±0.059, 0.370±0.065) (p<0.05); The expression of p53 has correlation with histological differentiating degree and lymph node metastasis (p<0.05), but it was irrelevant to the age, tumor size and clinical grade (p>0.05). The expression of S100A4 has correlation with clinical grade and lymph node metastasis (p<0.05), but no association with the age, tumor size and histological differentiating degree was observed in this study (p>0.05). The expressions of PTEN has correlation with lymph node metastasis p<0.05, but no association with the age, tumor size, clinical grade and histological differentiating degree was noted in our study (p>0.05). Conclusion: Expression of P53, PTEN and S100A4 can be expected to be taken as to the prognosis biomarkers of breast cancer and potential targets for new and effective treatment strategies for breast cancer.https://www.nepjol.info/index.php/AJMS/article/view/12599invasive ductal breast cancerp53pten s100a4immunohistochemistrywestern blot |
| spellingShingle | Zhang Gang Expression of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significance Asian Journal of Medical Sciences invasive ductal breast cancer p53 pten s100a4 immunohistochemistry western blot |
| title | Expression of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significance |
| title_full | Expression of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significance |
| title_fullStr | Expression of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significance |
| title_full_unstemmed | Expression of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significance |
| title_short | Expression of P53, PTEN and S100A4 in invasive ductal breast cancer and the clinical significance |
| title_sort | expression of p53 pten and s100a4 in invasive ductal breast cancer and the clinical significance |
| topic | invasive ductal breast cancer p53 pten s100a4 immunohistochemistry western blot |
| url | https://www.nepjol.info/index.php/AJMS/article/view/12599 |
| work_keys_str_mv | AT zhanggang expressionofp53ptenands100a4ininvasiveductalbreastcancerandtheclinicalsignificance |