Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators
Optogenetic (photo-responsive) actuators engineered from photoreceptors are widely used in various applications to study cell biology and tissue physiology. In the toolkit of optogenetic actuators, the key building blocks are genetically encodable light-sensitive proteins. Currently, most optogeneti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/18/6522 |
| _version_ | 1797554426549895168 |
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| author | Xiaocen Lu Yi Shen Robert E. Campbell |
| author_facet | Xiaocen Lu Yi Shen Robert E. Campbell |
| author_sort | Xiaocen Lu |
| collection | DOAJ |
| description | Optogenetic (photo-responsive) actuators engineered from photoreceptors are widely used in various applications to study cell biology and tissue physiology. In the toolkit of optogenetic actuators, the key building blocks are genetically encodable light-sensitive proteins. Currently, most optogenetic photosensory modules are engineered from naturally-occurring photoreceptor proteins from bacteria, fungi, and plants. There is a growing demand for novel photosensory domains with improved optical properties and light-induced responses to satisfy the needs of a wider variety of studies in biological sciences. In this review, we focus on progress towards engineering of non-opsin-based photosensory domains, and their representative applications in cell biology and physiology. We summarize current knowledge of engineering of light-sensitive proteins including light-oxygen-voltage-sensing domain (LOV), cryptochrome (CRY2), phytochrome (PhyB and BphP), and fluorescent protein (FP)-based photosensitive domains (Dronpa and PhoCl). |
| first_indexed | 2024-03-10T16:31:53Z |
| format | Article |
| id | doaj.art-6a49391d314642349c6a59bf4c90e62d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T16:31:53Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6a49391d314642349c6a59bf4c90e62d2023-11-20T12:47:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-012118652210.3390/ijms21186522Engineering Photosensory Modules of Non-Opsin-Based Optogenetic ActuatorsXiaocen Lu0Yi Shen1Robert E. Campbell2Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, CanadaDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, CanadaDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, CanadaOptogenetic (photo-responsive) actuators engineered from photoreceptors are widely used in various applications to study cell biology and tissue physiology. In the toolkit of optogenetic actuators, the key building blocks are genetically encodable light-sensitive proteins. Currently, most optogenetic photosensory modules are engineered from naturally-occurring photoreceptor proteins from bacteria, fungi, and plants. There is a growing demand for novel photosensory domains with improved optical properties and light-induced responses to satisfy the needs of a wider variety of studies in biological sciences. In this review, we focus on progress towards engineering of non-opsin-based photosensory domains, and their representative applications in cell biology and physiology. We summarize current knowledge of engineering of light-sensitive proteins including light-oxygen-voltage-sensing domain (LOV), cryptochrome (CRY2), phytochrome (PhyB and BphP), and fluorescent protein (FP)-based photosensitive domains (Dronpa and PhoCl).https://www.mdpi.com/1422-0067/21/18/6522optogenetic actuatorprotein engineeringphotosensory domains |
| spellingShingle | Xiaocen Lu Yi Shen Robert E. Campbell Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators International Journal of Molecular Sciences optogenetic actuator protein engineering photosensory domains |
| title | Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators |
| title_full | Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators |
| title_fullStr | Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators |
| title_full_unstemmed | Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators |
| title_short | Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators |
| title_sort | engineering photosensory modules of non opsin based optogenetic actuators |
| topic | optogenetic actuator protein engineering photosensory domains |
| url | https://www.mdpi.com/1422-0067/21/18/6522 |
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