Imaging of Indocyanine Green-Human Serum Albumin (ICG-HSA) Complex in Secreted Protein Acidic and Rich in Cysteine (SPARC)-Expressing Glioblastoma
Glioblastoma is the most common and fatal primary glioma and has a severe prognosis. It is a challenge for neurosurgeons to remove brain tumor tissues completely by resection. Meanwhile, fluorescence-guided surgery (FGS) is a technique used in glioma surgery to enhance the visualization of tumor edg...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/1/850 |
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| author | Hye Jung Jang Myung Geun Song Cho Rong Park Hyewon Youn Yun-Sang Lee Gi Jeong Cheon Keon Wook Kang |
| author_facet | Hye Jung Jang Myung Geun Song Cho Rong Park Hyewon Youn Yun-Sang Lee Gi Jeong Cheon Keon Wook Kang |
| author_sort | Hye Jung Jang |
| collection | DOAJ |
| description | Glioblastoma is the most common and fatal primary glioma and has a severe prognosis. It is a challenge for neurosurgeons to remove brain tumor tissues completely by resection. Meanwhile, fluorescence-guided surgery (FGS) is a technique used in glioma surgery to enhance the visualization of tumor edges to clarify the extent of tumor resection. Indocyanine green (ICG) is the only FDA-approved NIR fluorescent agent. It non-covalently binds to human serum albumin (HSA). Secreted protein acidic and rich in cysteine (SPARC) is an extracellular glycoprotein expressed in gliomas and binds to albumin, suggesting that it plays an important role in tumor uptake of the ICG-HSA complex. Here we demonstrate the binding properties of HSA or SPARC to ICG using surface plasmon resonance and saturation binding assay. According to in vitro and in vivo studies, the results showed that the uptake of ICG-HSA complex was higher in SPARC-expressing glioblastoma cell line and tumor region compared with the uptake of free ICG. Here, we visualized the SPARC-dependent uptake of ICG and ICG-HSA complex in U87MG. Our results demonstrated that the ICG-HSA complex is likely to be used as an efficient imaging agent targeting SPARC-expressing tumors, especially glioblastoma. |
| first_indexed | 2024-03-11T09:57:52Z |
| format | Article |
| id | doaj.art-6a53ba94a0df41ccb081355ee8e51dc9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:57:52Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6a53ba94a0df41ccb081355ee8e51dc92023-11-16T15:39:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-0124185010.3390/ijms24010850Imaging of Indocyanine Green-Human Serum Albumin (ICG-HSA) Complex in Secreted Protein Acidic and Rich in Cysteine (SPARC)-Expressing GlioblastomaHye Jung Jang0Myung Geun Song1Cho Rong Park2Hyewon Youn3Yun-Sang Lee4Gi Jeong Cheon5Keon Wook Kang6Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of KoreaDepartment of Nuclear Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of KoreaDepartment of Nuclear Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of KoreaDepartment of Nuclear Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of KoreaDepartment of Nuclear Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of KoreaDepartment of Nuclear Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of KoreaDepartment of Nuclear Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of KoreaGlioblastoma is the most common and fatal primary glioma and has a severe prognosis. It is a challenge for neurosurgeons to remove brain tumor tissues completely by resection. Meanwhile, fluorescence-guided surgery (FGS) is a technique used in glioma surgery to enhance the visualization of tumor edges to clarify the extent of tumor resection. Indocyanine green (ICG) is the only FDA-approved NIR fluorescent agent. It non-covalently binds to human serum albumin (HSA). Secreted protein acidic and rich in cysteine (SPARC) is an extracellular glycoprotein expressed in gliomas and binds to albumin, suggesting that it plays an important role in tumor uptake of the ICG-HSA complex. Here we demonstrate the binding properties of HSA or SPARC to ICG using surface plasmon resonance and saturation binding assay. According to in vitro and in vivo studies, the results showed that the uptake of ICG-HSA complex was higher in SPARC-expressing glioblastoma cell line and tumor region compared with the uptake of free ICG. Here, we visualized the SPARC-dependent uptake of ICG and ICG-HSA complex in U87MG. Our results demonstrated that the ICG-HSA complex is likely to be used as an efficient imaging agent targeting SPARC-expressing tumors, especially glioblastoma.https://www.mdpi.com/1422-0067/24/1/850glioblastomafluorescence-guided surgerysecreted protein acidic and rich in cysteine (SPARC)human serum albuminindocyanine green |
| spellingShingle | Hye Jung Jang Myung Geun Song Cho Rong Park Hyewon Youn Yun-Sang Lee Gi Jeong Cheon Keon Wook Kang Imaging of Indocyanine Green-Human Serum Albumin (ICG-HSA) Complex in Secreted Protein Acidic and Rich in Cysteine (SPARC)-Expressing Glioblastoma International Journal of Molecular Sciences glioblastoma fluorescence-guided surgery secreted protein acidic and rich in cysteine (SPARC) human serum albumin indocyanine green |
| title | Imaging of Indocyanine Green-Human Serum Albumin (ICG-HSA) Complex in Secreted Protein Acidic and Rich in Cysteine (SPARC)-Expressing Glioblastoma |
| title_full | Imaging of Indocyanine Green-Human Serum Albumin (ICG-HSA) Complex in Secreted Protein Acidic and Rich in Cysteine (SPARC)-Expressing Glioblastoma |
| title_fullStr | Imaging of Indocyanine Green-Human Serum Albumin (ICG-HSA) Complex in Secreted Protein Acidic and Rich in Cysteine (SPARC)-Expressing Glioblastoma |
| title_full_unstemmed | Imaging of Indocyanine Green-Human Serum Albumin (ICG-HSA) Complex in Secreted Protein Acidic and Rich in Cysteine (SPARC)-Expressing Glioblastoma |
| title_short | Imaging of Indocyanine Green-Human Serum Albumin (ICG-HSA) Complex in Secreted Protein Acidic and Rich in Cysteine (SPARC)-Expressing Glioblastoma |
| title_sort | imaging of indocyanine green human serum albumin icg hsa complex in secreted protein acidic and rich in cysteine sparc expressing glioblastoma |
| topic | glioblastoma fluorescence-guided surgery secreted protein acidic and rich in cysteine (SPARC) human serum albumin indocyanine green |
| url | https://www.mdpi.com/1422-0067/24/1/850 |
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