The effects of adipose tissue-derived stem cells seeded onto the curcumin-loaded collagen scaffold in healing of experimentally induced oral mucosal ulcers in rat
Objective(s): Various therapeutic approaches, including stem-cell-based strategies and tissue engineering, have been proposed for oral ulcerative lesions. We investigated the effects of adipose tissue-derived stem cells (ADSCs) seeded onto the curcumin-loaded collagen scaffold in the mucosal healing...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2020-12-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_16734_db91f2230dae35105293bb6d7e84677c.pdf |
| _version_ | 1811281472393314304 |
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| author | Maryam Mardani Azita Sadeghzadeh Nader Tanideh Azadeh Andisheh Tadbir Fatemeh Lavaee Moein Zarei Javad Moayedi |
| author_facet | Maryam Mardani Azita Sadeghzadeh Nader Tanideh Azadeh Andisheh Tadbir Fatemeh Lavaee Moein Zarei Javad Moayedi |
| author_sort | Maryam Mardani |
| collection | DOAJ |
| description | Objective(s): Various therapeutic approaches, including stem-cell-based strategies and tissue engineering, have been proposed for oral ulcerative lesions. We investigated the effects of adipose tissue-derived stem cells (ADSCs) seeded onto the curcumin-loaded collagen scaffold in the mucosal healing of oral ulcers in rats.Materials and Methods: The current experimental study was conducted on 40 male Sprague-Dawley rats. Oral ulcers were created over both sides of buccal mucosa, and the rats were randomly divided into four equal groups: 1) an untreated group (negative control); 2) Teriadent-treated group (positive control); 3) group treated with curcumin-loaded collagen scaffold; and 4) group received the ADSCs (3 × 106 cells) seeded onto the curcumin-loaded collagen scaffold. Rats were sacrificed on 3rd and 7th day after ulceration for histopathological examination as well as measurement of tissue levels of myeloperoxidase (MPO), superoxide dismutase (SOD), and Interleukin-1 beta (IL-1β) activity. Results: Compared with the negative control, the tissue levels of MPO and IL-1β were significantly decreased in all treated groups (p <0.0001); however, the SOD activity was elevated (p <0.0001). The highest SOD activity as well as the lowest MPO and IL-1β levels were observed in the ADSCs-curcumin-loaded collagen scaffold group. The ulcer healing process at 3rd and 7th day follow-up was much more progressed in the ADSCs-curcumin-loaded collagen scaffold group in comparison with the untreated group (P=0.037 and P=0.004, respectively).Conclusion: According to the findings of this study, ADSCs seeded onto the curcumin-loaded collagen scaffold seems to have a promising potential for oral ulcer healing applications. |
| first_indexed | 2024-04-13T01:33:16Z |
| format | Article |
| id | doaj.art-6a53e54b31d94351b00461200a240a1a |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-13T01:33:16Z |
| publishDate | 2020-12-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-6a53e54b31d94351b00461200a240a1a2022-12-22T03:08:27ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742020-12-0123121618162710.22038/ijbms.2020.48698.1117116734The effects of adipose tissue-derived stem cells seeded onto the curcumin-loaded collagen scaffold in healing of experimentally induced oral mucosal ulcers in ratMaryam Mardani0Azita Sadeghzadeh1Nader Tanideh2Azadeh Andisheh Tadbir3Fatemeh Lavaee4Moein Zarei5Javad Moayedi6Oral and Dental Disease Research Center, Department of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, IranPostgraduate Student, Oral and Dental Disease Research Center, Department of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, IranStem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranOral and Dental Disease Research Center, Department of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, IranOral and Dental Disease Research Center, Department of Oral and Maxillofacial Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, IranWest Pomeranian University of Technology, Szczecin, Department of Polymer and Biomaterials Science, Al. Piastow 45, 71-311 Szczecin, PolandDiagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IranObjective(s): Various therapeutic approaches, including stem-cell-based strategies and tissue engineering, have been proposed for oral ulcerative lesions. We investigated the effects of adipose tissue-derived stem cells (ADSCs) seeded onto the curcumin-loaded collagen scaffold in the mucosal healing of oral ulcers in rats.Materials and Methods: The current experimental study was conducted on 40 male Sprague-Dawley rats. Oral ulcers were created over both sides of buccal mucosa, and the rats were randomly divided into four equal groups: 1) an untreated group (negative control); 2) Teriadent-treated group (positive control); 3) group treated with curcumin-loaded collagen scaffold; and 4) group received the ADSCs (3 × 106 cells) seeded onto the curcumin-loaded collagen scaffold. Rats were sacrificed on 3rd and 7th day after ulceration for histopathological examination as well as measurement of tissue levels of myeloperoxidase (MPO), superoxide dismutase (SOD), and Interleukin-1 beta (IL-1β) activity. Results: Compared with the negative control, the tissue levels of MPO and IL-1β were significantly decreased in all treated groups (p <0.0001); however, the SOD activity was elevated (p <0.0001). The highest SOD activity as well as the lowest MPO and IL-1β levels were observed in the ADSCs-curcumin-loaded collagen scaffold group. The ulcer healing process at 3rd and 7th day follow-up was much more progressed in the ADSCs-curcumin-loaded collagen scaffold group in comparison with the untreated group (P=0.037 and P=0.004, respectively).Conclusion: According to the findings of this study, ADSCs seeded onto the curcumin-loaded collagen scaffold seems to have a promising potential for oral ulcer healing applications.https://ijbms.mums.ac.ir/article_16734_db91f2230dae35105293bb6d7e84677c.pdfadscscollagencurcuminexperimentaloral ulcersscaffoldulcer healing |
| spellingShingle | Maryam Mardani Azita Sadeghzadeh Nader Tanideh Azadeh Andisheh Tadbir Fatemeh Lavaee Moein Zarei Javad Moayedi The effects of adipose tissue-derived stem cells seeded onto the curcumin-loaded collagen scaffold in healing of experimentally induced oral mucosal ulcers in rat Iranian Journal of Basic Medical Sciences adscs collagen curcumin experimental oral ulcers scaffold ulcer healing |
| title | The effects of adipose tissue-derived stem cells seeded onto the curcumin-loaded collagen scaffold in healing of experimentally induced oral mucosal ulcers in rat |
| title_full | The effects of adipose tissue-derived stem cells seeded onto the curcumin-loaded collagen scaffold in healing of experimentally induced oral mucosal ulcers in rat |
| title_fullStr | The effects of adipose tissue-derived stem cells seeded onto the curcumin-loaded collagen scaffold in healing of experimentally induced oral mucosal ulcers in rat |
| title_full_unstemmed | The effects of adipose tissue-derived stem cells seeded onto the curcumin-loaded collagen scaffold in healing of experimentally induced oral mucosal ulcers in rat |
| title_short | The effects of adipose tissue-derived stem cells seeded onto the curcumin-loaded collagen scaffold in healing of experimentally induced oral mucosal ulcers in rat |
| title_sort | effects of adipose tissue derived stem cells seeded onto the curcumin loaded collagen scaffold in healing of experimentally induced oral mucosal ulcers in rat |
| topic | adscs collagen curcumin experimental oral ulcers scaffold ulcer healing |
| url | https://ijbms.mums.ac.ir/article_16734_db91f2230dae35105293bb6d7e84677c.pdf |
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