In Vitro and In Vivo Potential of RH Strain of Toxoplasma Gondii (Type Ι) in Tissue Cyst Forming
Background: Based on recent studies, there are controversial reports on the capacity of tissue cyst forming of Toxoplasma gondii RH strain. In this study, the capacity was evaluated by in vivo and in vitro experiments.Methods: RH strain was subcutaneously inoculated to ten Wistar rats. After one m...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2013-09-01
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| Series: | Iranian Journal of Parasitology |
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| Online Access: | http://ijpa.tums.ac.ir/index.php/ijpa/article/view/734/421 |
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| author | Qasem AsgariI Hossein Keshavarz Saeedeh Shojaee Mohammad Hossein Motazedian Mehdi Mohebali Ramin MIRI Davood Mehrabani Mostafa Rezaeian |
| author_facet | Qasem AsgariI Hossein Keshavarz Saeedeh Shojaee Mohammad Hossein Motazedian Mehdi Mohebali Ramin MIRI Davood Mehrabani Mostafa Rezaeian |
| author_sort | Qasem AsgariI |
| collection | DOAJ |
| description | Background: Based on recent studies, there are controversial reports on the capacity of tissue cyst forming of Toxoplasma gondii RH strain. In this study, the capacity was evaluated by in vivo and in vitro experiments.Methods: RH strain was subcutaneously inoculated to ten Wistar rats. After one month, their blood, brain, tongue and diaphragm were collected and evaluated by MAT, PCR, pathological and bioassay methods. The parasite was cultivated in the cell monolayer. To change to bradyzoite, the media pH was altered to 6.8. Biological aspect of the bradyzoites was evaluated by incubation in acidic pepsin and it's inoculation in ten BALB/c mice.Results: All rats showed antibodies to Toxoplasma at titers ≥1:320 but no DNA and tissue cyst were detected in the tissues. Following intraperitoneal inoculation of rats’ brain homogenate into BALB/c mice, no infection was established in none of the animals. During presence of cell culture, in acid media for a 3-5 days period, cyst-like structures were noticed when they were stained with PAS. The visible bradyzoites in the cysts that were incubated in acid pepsin medium were not able to kill any mice.Conclusion: This study confirmed that Iranian RH strain has lost the potential of tissue cyst forming in rats and bradyzoites cultivated in cell culture lost their resistance to acidic condition, so this strain can be a candidate for future vaccine researches. |
| first_indexed | 2024-12-13T18:42:15Z |
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| id | doaj.art-6a53ea27f55a47f3bcfe1397451e7a08 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-13T18:42:15Z |
| publishDate | 2013-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-6a53ea27f55a47f3bcfe1397451e7a082022-12-21T23:35:11ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2013-09-0183367375In Vitro and In Vivo Potential of RH Strain of Toxoplasma Gondii (Type Ι) in Tissue Cyst FormingQasem AsgariIHossein KeshavarzSaeedeh ShojaeeMohammad Hossein MotazedianMehdi MohebaliRamin MIRIDavood MehrabaniMostafa RezaeianBackground: Based on recent studies, there are controversial reports on the capacity of tissue cyst forming of Toxoplasma gondii RH strain. In this study, the capacity was evaluated by in vivo and in vitro experiments.Methods: RH strain was subcutaneously inoculated to ten Wistar rats. After one month, their blood, brain, tongue and diaphragm were collected and evaluated by MAT, PCR, pathological and bioassay methods. The parasite was cultivated in the cell monolayer. To change to bradyzoite, the media pH was altered to 6.8. Biological aspect of the bradyzoites was evaluated by incubation in acidic pepsin and it's inoculation in ten BALB/c mice.Results: All rats showed antibodies to Toxoplasma at titers ≥1:320 but no DNA and tissue cyst were detected in the tissues. Following intraperitoneal inoculation of rats’ brain homogenate into BALB/c mice, no infection was established in none of the animals. During presence of cell culture, in acid media for a 3-5 days period, cyst-like structures were noticed when they were stained with PAS. The visible bradyzoites in the cysts that were incubated in acid pepsin medium were not able to kill any mice.Conclusion: This study confirmed that Iranian RH strain has lost the potential of tissue cyst forming in rats and bradyzoites cultivated in cell culture lost their resistance to acidic condition, so this strain can be a candidate for future vaccine researches.http://ijpa.tums.ac.ir/index.php/ijpa/article/view/734/421Toxoplasma GondiiRH StrainTissue CystIn VitroIn Vivo |
| spellingShingle | Qasem AsgariI Hossein Keshavarz Saeedeh Shojaee Mohammad Hossein Motazedian Mehdi Mohebali Ramin MIRI Davood Mehrabani Mostafa Rezaeian In Vitro and In Vivo Potential of RH Strain of Toxoplasma Gondii (Type Ι) in Tissue Cyst Forming Iranian Journal of Parasitology Toxoplasma Gondii RH Strain Tissue Cyst In Vitro In Vivo |
| title | In Vitro and In Vivo Potential of RH Strain of Toxoplasma Gondii (Type Ι) in Tissue Cyst Forming |
| title_full | In Vitro and In Vivo Potential of RH Strain of Toxoplasma Gondii (Type Ι) in Tissue Cyst Forming |
| title_fullStr | In Vitro and In Vivo Potential of RH Strain of Toxoplasma Gondii (Type Ι) in Tissue Cyst Forming |
| title_full_unstemmed | In Vitro and In Vivo Potential of RH Strain of Toxoplasma Gondii (Type Ι) in Tissue Cyst Forming |
| title_short | In Vitro and In Vivo Potential of RH Strain of Toxoplasma Gondii (Type Ι) in Tissue Cyst Forming |
| title_sort | in vitro and in vivo potential of rh strain of toxoplasma gondii type ι in tissue cyst forming |
| topic | Toxoplasma Gondii RH Strain Tissue Cyst In Vitro In Vivo |
| url | http://ijpa.tums.ac.ir/index.php/ijpa/article/view/734/421 |
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