In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation
Background/Aim: MK615 extracted from Prunus mume was reported to have anti-inflammatory effects. In this article, we examined the in vivo antitumor effect of MK615 (an extract from Japanese apricot) using mouse tumor xenografts and focusing on the downregulation of PD-L1 (programmed death-ligand 1),...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2018-09-01
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| Series: | Integrative Cancer Therapies |
| Online Access: | https://doi.org/10.1177/1534735418766403 |
| _version_ | 1819031666573705216 |
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| author | Masashi Yanaki MD Masayuki Kobayashi MD Atsushi Aruga MD Minoru Nomura MD Makoto Ozaki MD |
| author_facet | Masashi Yanaki MD Masayuki Kobayashi MD Atsushi Aruga MD Minoru Nomura MD Makoto Ozaki MD |
| author_sort | Masashi Yanaki MD |
| collection | DOAJ |
| description | Background/Aim: MK615 extracted from Prunus mume was reported to have anti-inflammatory effects. In this article, we examined the in vivo antitumor effect of MK615 (an extract from Japanese apricot) using mouse tumor xenografts and focusing on the downregulation of PD-L1 (programmed death-ligand 1), a ligand of programmed cell death-1, a surface protein of activated T cells. Materials and Methods: B16/BL6 melanoma cells were injected into C57BL/6 or BALB/c-nu/nu mice to establish lung metastasis. BALB/c-nu/nu mice (nude mice) were used as a T cell–deficient model. The mice were given MK615 or saline orally every other day for approximately 8 weeks, and their survival was observed. NF-κB (nuclear factor-κB) and PD-L1 expressions of metastatic lung tissues were also examined. Results: The survival rate was improved only in the MK615-treated C57BL/6 mice ( P < .05), not in the saline-given control mice or BALB/c-nu/nu mice. The downregulations of NF-κB and PD-L1 were observed in both MK615-treated C57BL/6 and BALB/c-nu/nu mice. These results suggest that the antitumor effects of MK615 are associated with T cell–mediated immunity activated by MK-615-induced PD-L1 downregulation in tumor cells. Conclusion: MK615 is beneficial for a prolonged host survival time in the B16/BL6 melanoma xenograft model associated with T cell–mediated antitumor immunity. |
| first_indexed | 2024-12-21T06:49:41Z |
| format | Article |
| id | doaj.art-6a56bc5a394e4da4b6cdbcd15e2fd8e7 |
| institution | Directory Open Access Journal |
| issn | 1552-695X |
| language | English |
| last_indexed | 2024-12-21T06:49:41Z |
| publishDate | 2018-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Integrative Cancer Therapies |
| spelling | doaj.art-6a56bc5a394e4da4b6cdbcd15e2fd8e72022-12-21T19:12:31ZengSAGE PublishingIntegrative Cancer Therapies1552-695X2018-09-011710.1177/1534735418766403In Vivo Antitumor Effects of MK615 Led by PD-L1 DownregulationMasashi Yanaki MD0Masayuki Kobayashi MD1Atsushi Aruga MD2Minoru Nomura MD3Makoto Ozaki MD4Tokyo Women’s Medical University, Tokyo, JapanTokyo Women’s Medical University, Tokyo, JapanTokyo Women’s Medical University, Tokyo, JapanTokyo Women’s Medical University, Tokyo, JapanTokyo Women’s Medical University, Tokyo, JapanBackground/Aim: MK615 extracted from Prunus mume was reported to have anti-inflammatory effects. In this article, we examined the in vivo antitumor effect of MK615 (an extract from Japanese apricot) using mouse tumor xenografts and focusing on the downregulation of PD-L1 (programmed death-ligand 1), a ligand of programmed cell death-1, a surface protein of activated T cells. Materials and Methods: B16/BL6 melanoma cells were injected into C57BL/6 or BALB/c-nu/nu mice to establish lung metastasis. BALB/c-nu/nu mice (nude mice) were used as a T cell–deficient model. The mice were given MK615 or saline orally every other day for approximately 8 weeks, and their survival was observed. NF-κB (nuclear factor-κB) and PD-L1 expressions of metastatic lung tissues were also examined. Results: The survival rate was improved only in the MK615-treated C57BL/6 mice ( P < .05), not in the saline-given control mice or BALB/c-nu/nu mice. The downregulations of NF-κB and PD-L1 were observed in both MK615-treated C57BL/6 and BALB/c-nu/nu mice. These results suggest that the antitumor effects of MK615 are associated with T cell–mediated immunity activated by MK-615-induced PD-L1 downregulation in tumor cells. Conclusion: MK615 is beneficial for a prolonged host survival time in the B16/BL6 melanoma xenograft model associated with T cell–mediated antitumor immunity.https://doi.org/10.1177/1534735418766403 |
| spellingShingle | Masashi Yanaki MD Masayuki Kobayashi MD Atsushi Aruga MD Minoru Nomura MD Makoto Ozaki MD In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation Integrative Cancer Therapies |
| title | In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation |
| title_full | In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation |
| title_fullStr | In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation |
| title_full_unstemmed | In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation |
| title_short | In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation |
| title_sort | in vivo antitumor effects of mk615 led by pd l1 downregulation |
| url | https://doi.org/10.1177/1534735418766403 |
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