PD-L1 Expression in Patients with Idiopathic Pulmonary Fibrosis
Background: Idiopathic pulmonary fibrosis (IPF) is the most common and severe form within the group of idiopathic interstitial pneumonias. It is characterized by repetitive alveolar injury in genetically susceptible individuals and abnormal wound healing, leading to dysregulated bronchiolar prolifer...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/10/23/5562 |
| _version_ | 1797507639424319488 |
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| author | Sissel Kronborg-White Line Bille Madsen Elisabeth Bendstrup Venerino Poletti |
| author_facet | Sissel Kronborg-White Line Bille Madsen Elisabeth Bendstrup Venerino Poletti |
| author_sort | Sissel Kronborg-White |
| collection | DOAJ |
| description | Background: Idiopathic pulmonary fibrosis (IPF) is the most common and severe form within the group of idiopathic interstitial pneumonias. It is characterized by repetitive alveolar injury in genetically susceptible individuals and abnormal wound healing, leading to dysregulated bronchiolar proliferation and excessive deposition of extracellular matrix, causing complete architectural distortion and fibrosis. Epithelial-to-mesenchymal transition is considered an important pathogenic event, a phenomenon also observed in various malignant neoplasms, in which tumor cells express programmed death-ligand one (PD-L1). The aim of this study was to assess the presence of PD-L1 in patients with IPF and other interstitial lung diseases (ILDs). Method: Patients with a clinically and radiologically suspected idiopathic interstitial pneumonia or other ILDs undergoing transbronchial cryobiopsy to confirm the diagnosis at the Department of Respiratory Diseases and Allergy, Aarhus University Hospital, were included in this prospective observational study. Cellular membrane PD-L1 expression in epithelial cells was determined using the DAKO PD-L1 IHC 22C3 PharmDx Kit. Results: Membrane-bound PD-L1 (mPD-L1) was found in twelve (28%) of the forty-three patients with IPF and in five (9%) of the fifty-five patients with other ILDs (<i>p</i> = 0.015). When adjusting for age, gender and smoking status, the odds ratio of having IPF when expressing mPD-L1 in alveolar and/or bronchiolar epithelial cells was 4.3 (CI: 1.3–14.3). Conclusion: Expression of mPD-L1 in epithelial cells in the lung parenchymal zones was detected in a consistent subgroup of patients with IPF compared to other interstitial pneumonias. Larger studies are needed to explore the role of mPD-L1 in patients with IPF. |
| first_indexed | 2024-03-10T04:51:22Z |
| format | Article |
| id | doaj.art-6a5927610c8f41c49363b0f0add67aa8 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T04:51:22Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-6a5927610c8f41c49363b0f0add67aa82023-11-23T02:35:58ZengMDPI AGJournal of Clinical Medicine2077-03832021-11-011023556210.3390/jcm10235562PD-L1 Expression in Patients with Idiopathic Pulmonary FibrosisSissel Kronborg-White0Line Bille Madsen1Elisabeth Bendstrup2Venerino Poletti3Center for Rare Lung Diseases, Department of Respiratory Diseases and Allergy, Aarhus University Hospital, 8200 Aarhus, DenmarkDepartment of Pathology, Aarhus University Hospital, 8200 Aarhus, DenmarkCenter for Rare Lung Diseases, Department of Respiratory Diseases and Allergy, Aarhus University Hospital, 8200 Aarhus, DenmarkCenter for Rare Lung Diseases, Department of Respiratory Diseases and Allergy, Aarhus University Hospital, 8200 Aarhus, DenmarkBackground: Idiopathic pulmonary fibrosis (IPF) is the most common and severe form within the group of idiopathic interstitial pneumonias. It is characterized by repetitive alveolar injury in genetically susceptible individuals and abnormal wound healing, leading to dysregulated bronchiolar proliferation and excessive deposition of extracellular matrix, causing complete architectural distortion and fibrosis. Epithelial-to-mesenchymal transition is considered an important pathogenic event, a phenomenon also observed in various malignant neoplasms, in which tumor cells express programmed death-ligand one (PD-L1). The aim of this study was to assess the presence of PD-L1 in patients with IPF and other interstitial lung diseases (ILDs). Method: Patients with a clinically and radiologically suspected idiopathic interstitial pneumonia or other ILDs undergoing transbronchial cryobiopsy to confirm the diagnosis at the Department of Respiratory Diseases and Allergy, Aarhus University Hospital, were included in this prospective observational study. Cellular membrane PD-L1 expression in epithelial cells was determined using the DAKO PD-L1 IHC 22C3 PharmDx Kit. Results: Membrane-bound PD-L1 (mPD-L1) was found in twelve (28%) of the forty-three patients with IPF and in five (9%) of the fifty-five patients with other ILDs (<i>p</i> = 0.015). When adjusting for age, gender and smoking status, the odds ratio of having IPF when expressing mPD-L1 in alveolar and/or bronchiolar epithelial cells was 4.3 (CI: 1.3–14.3). Conclusion: Expression of mPD-L1 in epithelial cells in the lung parenchymal zones was detected in a consistent subgroup of patients with IPF compared to other interstitial pneumonias. Larger studies are needed to explore the role of mPD-L1 in patients with IPF.https://www.mdpi.com/2077-0383/10/23/5562idiopathic pulmonary fibrosiscryobiopsyPD-L1 |
| spellingShingle | Sissel Kronborg-White Line Bille Madsen Elisabeth Bendstrup Venerino Poletti PD-L1 Expression in Patients with Idiopathic Pulmonary Fibrosis Journal of Clinical Medicine idiopathic pulmonary fibrosis cryobiopsy PD-L1 |
| title | PD-L1 Expression in Patients with Idiopathic Pulmonary Fibrosis |
| title_full | PD-L1 Expression in Patients with Idiopathic Pulmonary Fibrosis |
| title_fullStr | PD-L1 Expression in Patients with Idiopathic Pulmonary Fibrosis |
| title_full_unstemmed | PD-L1 Expression in Patients with Idiopathic Pulmonary Fibrosis |
| title_short | PD-L1 Expression in Patients with Idiopathic Pulmonary Fibrosis |
| title_sort | pd l1 expression in patients with idiopathic pulmonary fibrosis |
| topic | idiopathic pulmonary fibrosis cryobiopsy PD-L1 |
| url | https://www.mdpi.com/2077-0383/10/23/5562 |
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