Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative element
Abstract Background Pseudomonas aeruginosa is a cause of nosocomial infections, especially in patients with cystic fibrosis and burn wounds. PAO1 strain and its derivatives are widely used to study the biology of this bacterium, however recent studies demonstrated differences in the genomes and phen...
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-019-6378-6 |
| _version_ | 1819279058853167104 |
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| author | Adam Kawalek Karolina Kotecka Magdalena Modrzejewska Jan Gawor Grazyna Jagura-Burdzy Aneta Agnieszka Bartosik |
| author_facet | Adam Kawalek Karolina Kotecka Magdalena Modrzejewska Jan Gawor Grazyna Jagura-Burdzy Aneta Agnieszka Bartosik |
| author_sort | Adam Kawalek |
| collection | DOAJ |
| description | Abstract Background Pseudomonas aeruginosa is a cause of nosocomial infections, especially in patients with cystic fibrosis and burn wounds. PAO1 strain and its derivatives are widely used to study the biology of this bacterium, however recent studies demonstrated differences in the genomes and phenotypes of derivatives from different laboratories. Results Here we report the genome sequence of P. aeruginosa PAO1161 laboratory strain, a leu-, RifR, restriction-modification defective PAO1 derivative, described as the host of IncP-8 plasmid FP2, conferring the resistance to mercury. Comparison of PAO1161 genome with PAO1-UW sequence revealed lack of an inversion of a large genome segment between rRNA operons and 100 nucleotide polymorphisms, short insertions and deletions. These included a change in leuA, resulting in E108K substitution, which caused leucine auxotrophy and a mutation in rpoB, likely responsible for the rifampicin resistance. Nonsense mutations were detected in PA2735 and PA1939 encoding a DNA methyltransferase and a putative OLD family endonuclease, respectively. Analysis of revertants in these two genes showed that PA2735 is a component of a restriction-modification system, independent of PA1939. Moreover, a 12 kb RPG42 prophage and a novel 108 kb PAPI-1 like integrative conjugative element (ICE) encompassing a mercury resistance operon were identified. The ICEPae1161 was transferred to Pseudomonas putida cells, where it integrated in the genome and conferred the mercury resistance. Conclusions The high-quality P. aeruginosa PAO1161 genome sequence provides a reference for further research including e.g. investigation of horizontal gene transfer or comparative genomics. The strain was found to carry ICEPae1161, a functional PAPI-1 family integrative conjugative element, containing loci conferring mercury resistance, in the past attributed to the FP2 plasmid of IncP-8 incompatibility group. This indicates that the only known member of IncP-8 is in fact an ICE. |
| first_indexed | 2024-12-24T00:21:52Z |
| format | Article |
| id | doaj.art-6a5b23252a3e41f89b3fd82d7aa346e5 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-24T00:21:52Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
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| series | BMC Genomics |
| spelling | doaj.art-6a5b23252a3e41f89b3fd82d7aa346e52022-12-21T17:24:33ZengBMCBMC Genomics1471-21642020-01-0121111210.1186/s12864-019-6378-6Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative elementAdam Kawalek0Karolina Kotecka1Magdalena Modrzejewska2Jan Gawor3Grazyna Jagura-Burdzy4Aneta Agnieszka Bartosik5Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Department of Microbial BiochemistryInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Department of Microbial BiochemistryInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Department of Microbial BiochemistryInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, DNA Sequencing and Oligonucleotide Synthesis LaboratoryInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Department of Microbial BiochemistryInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Department of Microbial BiochemistryAbstract Background Pseudomonas aeruginosa is a cause of nosocomial infections, especially in patients with cystic fibrosis and burn wounds. PAO1 strain and its derivatives are widely used to study the biology of this bacterium, however recent studies demonstrated differences in the genomes and phenotypes of derivatives from different laboratories. Results Here we report the genome sequence of P. aeruginosa PAO1161 laboratory strain, a leu-, RifR, restriction-modification defective PAO1 derivative, described as the host of IncP-8 plasmid FP2, conferring the resistance to mercury. Comparison of PAO1161 genome with PAO1-UW sequence revealed lack of an inversion of a large genome segment between rRNA operons and 100 nucleotide polymorphisms, short insertions and deletions. These included a change in leuA, resulting in E108K substitution, which caused leucine auxotrophy and a mutation in rpoB, likely responsible for the rifampicin resistance. Nonsense mutations were detected in PA2735 and PA1939 encoding a DNA methyltransferase and a putative OLD family endonuclease, respectively. Analysis of revertants in these two genes showed that PA2735 is a component of a restriction-modification system, independent of PA1939. Moreover, a 12 kb RPG42 prophage and a novel 108 kb PAPI-1 like integrative conjugative element (ICE) encompassing a mercury resistance operon were identified. The ICEPae1161 was transferred to Pseudomonas putida cells, where it integrated in the genome and conferred the mercury resistance. Conclusions The high-quality P. aeruginosa PAO1161 genome sequence provides a reference for further research including e.g. investigation of horizontal gene transfer or comparative genomics. The strain was found to carry ICEPae1161, a functional PAPI-1 family integrative conjugative element, containing loci conferring mercury resistance, in the past attributed to the FP2 plasmid of IncP-8 incompatibility group. This indicates that the only known member of IncP-8 is in fact an ICE.https://doi.org/10.1186/s12864-019-6378-6Pseudomonas aeruginosaGenome sequenceIntegrative conjugative elementMercury resistance |
| spellingShingle | Adam Kawalek Karolina Kotecka Magdalena Modrzejewska Jan Gawor Grazyna Jagura-Burdzy Aneta Agnieszka Bartosik Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative element BMC Genomics Pseudomonas aeruginosa Genome sequence Integrative conjugative element Mercury resistance |
| title | Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative element |
| title_full | Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative element |
| title_fullStr | Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative element |
| title_full_unstemmed | Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative element |
| title_short | Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative element |
| title_sort | genome sequence of pseudomonas aeruginosa pao1161 a pao1 derivative with the icepae1161 integrative and conjugative element |
| topic | Pseudomonas aeruginosa Genome sequence Integrative conjugative element Mercury resistance |
| url | https://doi.org/10.1186/s12864-019-6378-6 |
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