Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast <i>Geotrichum candidum</i> PO27
Enzymes are biocatalysts mainly used for their industrial potential in various applications. The present study aims to understand the enzyme production for biotechnological interest from a local yeast strain. From 100 isolates obtained from various biotopes, 78 strains were selected for their enzyma...
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AIMS Press
2024-01-01
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| Series: | AIMS Microbiology |
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| Online Access: | https://www.aimspress.com/article/doi/10.3934/microbiol.2024006?viewType=HTML |
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| author | Ibtissem Chaib Scheherazed Dakhmouche-Djekrif Leila Bennamoun Tahar Nouadri |
| author_facet | Ibtissem Chaib Scheherazed Dakhmouche-Djekrif Leila Bennamoun Tahar Nouadri |
| author_sort | Ibtissem Chaib |
| collection | DOAJ |
| description | Enzymes are biocatalysts mainly used for their industrial potential in various applications. The present study aims to understand the enzyme production for biotechnological interest from a local yeast strain. From 100 isolates obtained from various biotopes, 78 strains were selected for their enzymatic heritage. Screening of α-amylase, lipase/esterase, and cellulase activities by rapid plate detection methods was carried out and the PO27 yeast was selected for its high capacity to produce α-amylase. In addition, this yeast strain exhibited good lipolytic and esterolytic activities, as well as low cellulase activity. A sequence analysis of the D1/D2 region of the 26S ribosomal RNA (26S rRNA) and a study of morphological characteristics identified the PO27 strain as <italic>Geotrichum candidum</italic>. The production of α-amylase has been studied in solid medium fermentation using various natural substrates without any supplementation such as olive pomace, potato peels, leftover bread, and mastic cake. <italic>G. candidum</italic> PO27 showed an improved production of α-amylase with olive pomace, thus reaching approximately 180.71 U/g. To evaluate the ability of this isolate to produce α-amylase in submerged fermentation, multiple concentrations of olive pomace substrate were tested. The best activity of submerged fermentation was statistically compared to the solid-state fermentation result in order to select the appropriate fermentation type. A high significant difference was found to rank the 6% olive pomace medium as the best substrate concentration with 34.395 U/mL of α-amylase activity. This work showed that the new isolate <italic>Geotrichum candidum</italic> PO27 has a better potential to produce α-amylase at a low cost in solid-state fermentation compared to submerged fermentation. Optimization conditions for PO27 α-amylase production through solid-state fermentation were achieved using a one factor at a time (OFAT) approach. The findings revealed that a high temperature (60 °C), an acidic pH, malt extract, and soluble starch were the highly significant medium components for enhancing α-amylase production. The use of olive pomace waste by <italic>Geotrichum candidum</italic> PO27 is expected to be effective in producing an industrially useful α-amylase. |
| first_indexed | 2024-04-24T10:59:44Z |
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| institution | Directory Open Access Journal |
| issn | 2471-1888 |
| language | English |
| last_indexed | 2024-04-24T10:59:44Z |
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| spelling | doaj.art-6a604f2b7a314bb8a1cac28ebba88d792024-04-12T01:27:07ZengAIMS PressAIMS Microbiology2471-18882024-01-011018310610.3934/microbiol.2024006Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast <i>Geotrichum candidum</i> PO27Ibtissem Chaib 0Scheherazed Dakhmouche-Djekrif 1Leila Bennamoun2Tahar Nouadri31. Laboratory of Microbiological Engineering and Applications, Department of Biochemistry and Molecular and Cellular Biology, Faculty of Natural and Life Sciences, Frères Mentouri University Constantine 1, Constantine 25017, Algeria1. Laboratory of Microbiological Engineering and Applications, Department of Biochemistry and Molecular and Cellular Biology, Faculty of Natural and Life Sciences, Frères Mentouri University Constantine 1, Constantine 25017, Algeria 2. Department of Natural Sciences, Teachers Training School El Katiba Assia Djebar, University town Ali Mendjeli, Constantine 25000, Algeria1. Laboratory of Microbiological Engineering and Applications, Department of Biochemistry and Molecular and Cellular Biology, Faculty of Natural and Life Sciences, Frères Mentouri University Constantine 1, Constantine 25017, Algeria1. Laboratory of Microbiological Engineering and Applications, Department of Biochemistry and Molecular and Cellular Biology, Faculty of Natural and Life Sciences, Frères Mentouri University Constantine 1, Constantine 25017, AlgeriaEnzymes are biocatalysts mainly used for their industrial potential in various applications. The present study aims to understand the enzyme production for biotechnological interest from a local yeast strain. From 100 isolates obtained from various biotopes, 78 strains were selected for their enzymatic heritage. Screening of α-amylase, lipase/esterase, and cellulase activities by rapid plate detection methods was carried out and the PO27 yeast was selected for its high capacity to produce α-amylase. In addition, this yeast strain exhibited good lipolytic and esterolytic activities, as well as low cellulase activity. A sequence analysis of the D1/D2 region of the 26S ribosomal RNA (26S rRNA) and a study of morphological characteristics identified the PO27 strain as <italic>Geotrichum candidum</italic>. The production of α-amylase has been studied in solid medium fermentation using various natural substrates without any supplementation such as olive pomace, potato peels, leftover bread, and mastic cake. <italic>G. candidum</italic> PO27 showed an improved production of α-amylase with olive pomace, thus reaching approximately 180.71 U/g. To evaluate the ability of this isolate to produce α-amylase in submerged fermentation, multiple concentrations of olive pomace substrate were tested. The best activity of submerged fermentation was statistically compared to the solid-state fermentation result in order to select the appropriate fermentation type. A high significant difference was found to rank the 6% olive pomace medium as the best substrate concentration with 34.395 U/mL of α-amylase activity. This work showed that the new isolate <italic>Geotrichum candidum</italic> PO27 has a better potential to produce α-amylase at a low cost in solid-state fermentation compared to submerged fermentation. Optimization conditions for PO27 α-amylase production through solid-state fermentation were achieved using a one factor at a time (OFAT) approach. The findings revealed that a high temperature (60 °C), an acidic pH, malt extract, and soluble starch were the highly significant medium components for enhancing α-amylase production. The use of olive pomace waste by <italic>Geotrichum candidum</italic> PO27 is expected to be effective in producing an industrially useful α-amylase.https://www.aimspress.com/article/doi/10.3934/microbiol.2024006?viewType=HTMLgeotrichum candidum po27α-amylaseolive pomacessfsmfofat |
| spellingShingle | Ibtissem Chaib Scheherazed Dakhmouche-Djekrif Leila Bennamoun Tahar Nouadri Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast <i>Geotrichum candidum</i> PO27 AIMS Microbiology geotrichum candidum po27 α-amylase olive pomace ssf smf ofat |
| title | Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast <i>Geotrichum candidum</i> PO27 |
| title_full | Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast <i>Geotrichum candidum</i> PO27 |
| title_fullStr | Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast <i>Geotrichum candidum</i> PO27 |
| title_full_unstemmed | Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast <i>Geotrichum candidum</i> PO27 |
| title_short | Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast <i>Geotrichum candidum</i> PO27 |
| title_sort | extracellular enzymes producing yeasts study cost effective production of α amylase by a newly isolated thermophilic yeast i geotrichum candidum i po27 |
| topic | geotrichum candidum po27 α-amylase olive pomace ssf smf ofat |
| url | https://www.aimspress.com/article/doi/10.3934/microbiol.2024006?viewType=HTML |
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