Characterization of C-strain “Riems” TAV-epitope escape variants obtained through selective antibody pressure in cell culture
<p>Abstract</p> <p>Classical swine fever virus (CSFV) C-strain “Riems” escape variants generated under selective antibody pressure with monoclonal antibodies and a peptide-specific antiserum in cell culture were investigated. Candidates with up to three amino acid exchanges in the...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-04-01
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| Series: | Veterinary Research |
| Online Access: | http://www.veterinaryresearch.org/content/43/1/33 |
| _version_ | 1811286370059026432 |
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| author | Leifer Immanuel Blome Sandra Blohm Ulrike König Patricia Küster Heike Lange Bodo Beer Martin |
| author_facet | Leifer Immanuel Blome Sandra Blohm Ulrike König Patricia Küster Heike Lange Bodo Beer Martin |
| author_sort | Leifer Immanuel |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Classical swine fever virus (CSFV) C-strain “Riems” escape variants generated under selective antibody pressure with monoclonal antibodies and a peptide-specific antiserum in cell culture were investigated. Candidates with up to three amino acid exchanges in the immunodominant and highly conserved linear TAV-epitope of the E2-glycoprotein, and additional mutations in the envelope proteins E<sup>RNS</sup> and E1, were characterized both in vitro and in vivo.</p> <p>It was further demonstrated, that intramuscular immunization of weaner pigs with variants selected after a series of passages elicited full protection against lethal CSFV challenge infection. These novel CSFV C-strain variants with exchanges in the TAV-epitope present potential marker vaccine candidates. The DIVA (differentiating infected from vaccinated animals) principle was tested for those variants using commercially available E2 antibody detection ELISA. Moreover, direct virus differentiation is possible using a real-time RT-PCR system specific for the new C-strain virus escape variants or using differential immunofluorescence staining.</p> |
| first_indexed | 2024-04-13T02:58:36Z |
| format | Article |
| id | doaj.art-6a61d973f51642108ebac29e8bb54333 |
| institution | Directory Open Access Journal |
| issn | 0928-4249 1297-9716 |
| language | English |
| last_indexed | 2024-04-13T02:58:36Z |
| publishDate | 2012-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-6a61d973f51642108ebac29e8bb543332022-12-22T03:05:31ZengBMCVeterinary Research0928-42491297-97162012-04-014313310.1186/1297-9716-43-33Characterization of C-strain “Riems” TAV-epitope escape variants obtained through selective antibody pressure in cell cultureLeifer ImmanuelBlome SandraBlohm UlrikeKönig PatriciaKüster HeikeLange BodoBeer Martin<p>Abstract</p> <p>Classical swine fever virus (CSFV) C-strain “Riems” escape variants generated under selective antibody pressure with monoclonal antibodies and a peptide-specific antiserum in cell culture were investigated. Candidates with up to three amino acid exchanges in the immunodominant and highly conserved linear TAV-epitope of the E2-glycoprotein, and additional mutations in the envelope proteins E<sup>RNS</sup> and E1, were characterized both in vitro and in vivo.</p> <p>It was further demonstrated, that intramuscular immunization of weaner pigs with variants selected after a series of passages elicited full protection against lethal CSFV challenge infection. These novel CSFV C-strain variants with exchanges in the TAV-epitope present potential marker vaccine candidates. The DIVA (differentiating infected from vaccinated animals) principle was tested for those variants using commercially available E2 antibody detection ELISA. Moreover, direct virus differentiation is possible using a real-time RT-PCR system specific for the new C-strain virus escape variants or using differential immunofluorescence staining.</p>http://www.veterinaryresearch.org/content/43/1/33 |
| spellingShingle | Leifer Immanuel Blome Sandra Blohm Ulrike König Patricia Küster Heike Lange Bodo Beer Martin Characterization of C-strain “Riems” TAV-epitope escape variants obtained through selective antibody pressure in cell culture Veterinary Research |
| title | Characterization of C-strain “Riems” TAV-epitope escape variants obtained through selective antibody pressure in cell culture |
| title_full | Characterization of C-strain “Riems” TAV-epitope escape variants obtained through selective antibody pressure in cell culture |
| title_fullStr | Characterization of C-strain “Riems” TAV-epitope escape variants obtained through selective antibody pressure in cell culture |
| title_full_unstemmed | Characterization of C-strain “Riems” TAV-epitope escape variants obtained through selective antibody pressure in cell culture |
| title_short | Characterization of C-strain “Riems” TAV-epitope escape variants obtained through selective antibody pressure in cell culture |
| title_sort | characterization of c strain riems tav epitope escape variants obtained through selective antibody pressure in cell culture |
| url | http://www.veterinaryresearch.org/content/43/1/33 |
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