Platelet and coagulation hemostasis status in patients with atrial fibrillation depending on warfarin dosing method
The aim. To evaluate indicators of induced platelet aggregation and D-dimer level in patients with atrial fibrillation (AF) depending on the method of warfarin (WF) dosing. Materials and methods. The study involved 110 patients with AF (mean age 68.72 ± 0.79; men – 57, women – 53). Patients with...
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| Format: | Article |
| Language: | English |
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Zaporozhye State Medical University
2022-12-01
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| Series: | Zaporožskij Medicinskij Žurnal |
| Subjects: | |
| Online Access: | http://zmj.zsmu.edu.ua/article/view/263895/266362 |
| _version_ | 1797957025136640000 |
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| author | M. Yu. Kolesnyk Ya. M. Mykhailovskyi |
| author_facet | M. Yu. Kolesnyk Ya. M. Mykhailovskyi |
| author_sort | M. Yu. Kolesnyk |
| collection | DOAJ |
| description | The aim. To evaluate indicators of induced platelet aggregation and D-dimer level in patients with atrial fibrillation (AF) depending on the method of warfarin (WF) dosing.
Materials and methods. The study involved 110 patients with AF (mean age 68.72 ± 0.79; men – 57, women – 53). Patients with AF were divided into two groups: the main group – 50 patients with AF and genotype-guided dosing method, the control group – 60 patients with AF and traditional dosing method. CYP2C9, CYP4F2, VKORC1 genetic polymorphisms were determined using multiplex real time polymerase chain reaction, D-dimer concentration – by the method of solid-phase enzyme immunoassay; ADP- and epinephrine-induced aggregation indicators – by the method of G. Born.
Results. The percentage (by 24 %), time (by 3 min 6 s) and rate in 30 s (by 19.5 %/min) of ADP-induced platelet aggregation were significantly lower in patients with AF and genotype-guided WF dosing method. D-dimer concentration in patients of the main and control groups did not differ significantly. However, significantly fewer patients with elevated D-dimer values were registered in the group with genotype-guided WF dosing method compared to the group with traditional dosing method: 0 (0.00 %) vs. 7 (11.67 %) at 500 ng FEU/ml cut-off (χ2 = 4.43, P < 0.05), 1 (2.00 %) vs. 9 (15.00 %) at 390 ng FEU/ml cut-off (χ2 = 4.16, P < 0.05), 0 (0.00 %) vs. 7 (11.67 %) at age-adjusted cut-off (χ2 = 4.43, P < 0.05).
Conclusions. The obtained results may indicate a potentially lower risk of thrombotic events in patients with AF and genotype-guided WF dosing method compared to the group with traditional dosing method, which confirms the benefit of the widespread use of the pharmacogenetic testing in clinical practice. |
| first_indexed | 2024-04-10T23:58:57Z |
| format | Article |
| id | doaj.art-6a62547e71eb48768032c9693a30ba31 |
| institution | Directory Open Access Journal |
| issn | 2306-4145 2310-1210 |
| language | English |
| last_indexed | 2024-04-10T23:58:57Z |
| publishDate | 2022-12-01 |
| publisher | Zaporozhye State Medical University |
| record_format | Article |
| series | Zaporožskij Medicinskij Žurnal |
| spelling | doaj.art-6a62547e71eb48768032c9693a30ba312023-01-10T08:56:15ZengZaporozhye State Medical UniversityZaporožskij Medicinskij Žurnal2306-41452310-12102022-12-0124664765110.14739/2310-1210.2022.6.263895Platelet and coagulation hemostasis status in patients with atrial fibrillation depending on warfarin dosing methodM. Yu. Kolesnyk0https://orcid.org/0000-0001-7566-1899Ya. M. Mykhailovskyi1https://orcid.org/0000-0002-1310-8585Zaporizhzhia State Medical University, UkraineZaporizhzhia State Medical University, UkraineThe aim. To evaluate indicators of induced platelet aggregation and D-dimer level in patients with atrial fibrillation (AF) depending on the method of warfarin (WF) dosing. Materials and methods. The study involved 110 patients with AF (mean age 68.72 ± 0.79; men – 57, women – 53). Patients with AF were divided into two groups: the main group – 50 patients with AF and genotype-guided dosing method, the control group – 60 patients with AF and traditional dosing method. CYP2C9, CYP4F2, VKORC1 genetic polymorphisms were determined using multiplex real time polymerase chain reaction, D-dimer concentration – by the method of solid-phase enzyme immunoassay; ADP- and epinephrine-induced aggregation indicators – by the method of G. Born. Results. The percentage (by 24 %), time (by 3 min 6 s) and rate in 30 s (by 19.5 %/min) of ADP-induced platelet aggregation were significantly lower in patients with AF and genotype-guided WF dosing method. D-dimer concentration in patients of the main and control groups did not differ significantly. However, significantly fewer patients with elevated D-dimer values were registered in the group with genotype-guided WF dosing method compared to the group with traditional dosing method: 0 (0.00 %) vs. 7 (11.67 %) at 500 ng FEU/ml cut-off (χ2 = 4.43, P < 0.05), 1 (2.00 %) vs. 9 (15.00 %) at 390 ng FEU/ml cut-off (χ2 = 4.16, P < 0.05), 0 (0.00 %) vs. 7 (11.67 %) at age-adjusted cut-off (χ2 = 4.43, P < 0.05). Conclusions. The obtained results may indicate a potentially lower risk of thrombotic events in patients with AF and genotype-guided WF dosing method compared to the group with traditional dosing method, which confirms the benefit of the widespread use of the pharmacogenetic testing in clinical practice.http://zmj.zsmu.edu.ua/article/view/263895/266362atrial fibrillationwarfarinplatelet aggregationd-dimergenotype-guided dosing |
| spellingShingle | M. Yu. Kolesnyk Ya. M. Mykhailovskyi Platelet and coagulation hemostasis status in patients with atrial fibrillation depending on warfarin dosing method Zaporožskij Medicinskij Žurnal atrial fibrillation warfarin platelet aggregation d-dimer genotype-guided dosing |
| title | Platelet and coagulation hemostasis status in patients with atrial fibrillation depending on warfarin dosing method |
| title_full | Platelet and coagulation hemostasis status in patients with atrial fibrillation depending on warfarin dosing method |
| title_fullStr | Platelet and coagulation hemostasis status in patients with atrial fibrillation depending on warfarin dosing method |
| title_full_unstemmed | Platelet and coagulation hemostasis status in patients with atrial fibrillation depending on warfarin dosing method |
| title_short | Platelet and coagulation hemostasis status in patients with atrial fibrillation depending on warfarin dosing method |
| title_sort | platelet and coagulation hemostasis status in patients with atrial fibrillation depending on warfarin dosing method |
| topic | atrial fibrillation warfarin platelet aggregation d-dimer genotype-guided dosing |
| url | http://zmj.zsmu.edu.ua/article/view/263895/266362 |
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