The superior healing capacity of MRL tendons is minimally influenced by the systemic environment of the MRL mouse
Abstract Murphy Roths Large mice (MRL) exhibit improved tendon healing and are often described as a “super-healer” strain. The underlying mechanisms that drive the superior healing response of MRL remain a controversial subject. We utilized a tendon transplantation model between MRL and “normal-heal...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-42449-8 |
| _version_ | 1797576977588158464 |
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| author | Borys Frankewycz Rebecca Bell Monideepa Chatterjee Nelly Andarawis-Puri |
| author_facet | Borys Frankewycz Rebecca Bell Monideepa Chatterjee Nelly Andarawis-Puri |
| author_sort | Borys Frankewycz |
| collection | DOAJ |
| description | Abstract Murphy Roths Large mice (MRL) exhibit improved tendon healing and are often described as a “super-healer” strain. The underlying mechanisms that drive the superior healing response of MRL remain a controversial subject. We utilized a tendon transplantation model between MRL and “normal-healer” B6-mice to differentiate between the contribution of MRL’s innate tendon and systemic environment to its improved healing capacity. Patellar tendons with a midsubstance punch injury were transplanted back into the same animal (autograft) or into an animal of the other strain (allograft). Findings at 4 weeks showed that the innate MRL tendon environment drives its improved healing capacity as demonstrated by improved stiffness and maximum load in MRL-grafts-in-B6-host-allografts compared to B6-autografts, and higher modulus in MRL-autografts compared to B6-graft-in-MRL-host-allografts. Groups with an MRL component showed an increase in pro-inflammatory cytokines in the 3 days after injury, suggesting an early enhanced inflammatory profile in MRL that ultimately resolves. A preserved range of motion of the knee joint in all MRL animals suggests a systemic “shielding effect” of MRL in regard to joint adhesiveness. Our findings 4-weeks post injury are consistent with previous studies showing tissue-driven improved healing and suggest that the systemic environment contributes to the overall healing process. |
| first_indexed | 2024-03-10T22:01:24Z |
| format | Article |
| id | doaj.art-6a65bd942f4d4ed5b0e9db0331676fe4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T22:01:24Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6a65bd942f4d4ed5b0e9db0331676fe42023-11-19T12:56:45ZengNature PortfolioScientific Reports2045-23222023-10-0113111310.1038/s41598-023-42449-8The superior healing capacity of MRL tendons is minimally influenced by the systemic environment of the MRL mouseBorys Frankewycz0Rebecca Bell1Monideepa Chatterjee2Nelly Andarawis-Puri3Sibley School of Mechanical and Aerospace Engineering, Cornell UniversitySibley School of Mechanical and Aerospace Engineering, Cornell UniversityMeinig School of Biomedical Engineering, Cornell UniversitySibley School of Mechanical and Aerospace Engineering, Cornell UniversityAbstract Murphy Roths Large mice (MRL) exhibit improved tendon healing and are often described as a “super-healer” strain. The underlying mechanisms that drive the superior healing response of MRL remain a controversial subject. We utilized a tendon transplantation model between MRL and “normal-healer” B6-mice to differentiate between the contribution of MRL’s innate tendon and systemic environment to its improved healing capacity. Patellar tendons with a midsubstance punch injury were transplanted back into the same animal (autograft) or into an animal of the other strain (allograft). Findings at 4 weeks showed that the innate MRL tendon environment drives its improved healing capacity as demonstrated by improved stiffness and maximum load in MRL-grafts-in-B6-host-allografts compared to B6-autografts, and higher modulus in MRL-autografts compared to B6-graft-in-MRL-host-allografts. Groups with an MRL component showed an increase in pro-inflammatory cytokines in the 3 days after injury, suggesting an early enhanced inflammatory profile in MRL that ultimately resolves. A preserved range of motion of the knee joint in all MRL animals suggests a systemic “shielding effect” of MRL in regard to joint adhesiveness. Our findings 4-weeks post injury are consistent with previous studies showing tissue-driven improved healing and suggest that the systemic environment contributes to the overall healing process.https://doi.org/10.1038/s41598-023-42449-8 |
| spellingShingle | Borys Frankewycz Rebecca Bell Monideepa Chatterjee Nelly Andarawis-Puri The superior healing capacity of MRL tendons is minimally influenced by the systemic environment of the MRL mouse Scientific Reports |
| title | The superior healing capacity of MRL tendons is minimally influenced by the systemic environment of the MRL mouse |
| title_full | The superior healing capacity of MRL tendons is minimally influenced by the systemic environment of the MRL mouse |
| title_fullStr | The superior healing capacity of MRL tendons is minimally influenced by the systemic environment of the MRL mouse |
| title_full_unstemmed | The superior healing capacity of MRL tendons is minimally influenced by the systemic environment of the MRL mouse |
| title_short | The superior healing capacity of MRL tendons is minimally influenced by the systemic environment of the MRL mouse |
| title_sort | superior healing capacity of mrl tendons is minimally influenced by the systemic environment of the mrl mouse |
| url | https://doi.org/10.1038/s41598-023-42449-8 |
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