Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds
Non-tuberculous mycobacteria (NTM) are considered a relevant cause of non-specific reactions to the most widely applied bovine tuberculosis (bTB) test, the intradermal tuberculin test. In order to establish which NTM species might act as a potential source of such diagnostic interference, a collecti...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1361788/full |
| _version_ | 1827368341777416192 |
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| author | Alberto Gomez-Buendia Alberto Gomez-Buendia Julio Alvarez Julio Alvarez Javier Bezos Javier Bezos Jorge Mourelo Javier Amado Jose Luis Saez Lucia de Juan Lucia de Juan Beatriz Romero Beatriz Romero |
| author_facet | Alberto Gomez-Buendia Alberto Gomez-Buendia Julio Alvarez Julio Alvarez Javier Bezos Javier Bezos Jorge Mourelo Javier Amado Jose Luis Saez Lucia de Juan Lucia de Juan Beatriz Romero Beatriz Romero |
| author_sort | Alberto Gomez-Buendia |
| collection | DOAJ |
| description | Non-tuberculous mycobacteria (NTM) are considered a relevant cause of non-specific reactions to the most widely applied bovine tuberculosis (bTB) test, the intradermal tuberculin test. In order to establish which NTM species might act as a potential source of such diagnostic interference, a collection of 373 isolates obtained from skin test positive cows from 359 officially tuberculosis-free (OTF) herds, culled in the framework of the bTB eradication campaign in Spain, were identified at the species level through PCR and Sanger sequencing of the 16S rDNA, hsp65 and rpoB genes. Of the 308 isolates for which a reliable identification was achieved, 32 different mycobacterial species were identified, with certain species being most represented: among M. avium complex members (n = 142, 46.1%), M. avium subsp. hominissuis (98; 69.0%) was the most abundant followed by M. avium subsp. avium (33, 23.2%), and M. intracellulare (7, 4.9%). Among non-MAC members (n = 166, 53.9%), M. nonchromogenicum (85; 27.6%) and M. bourgelatii (11; 5.6%) were the predominant species. In addition, mixed results were obtained in 53 isolates presenting up to 30 different genotypes, which could be indicative of new mycobacterial species. Our results represent a first step toward characterizing the diversity of NTM species that could interfere with official diagnostic tests for bTB eradication in Spain. |
| first_indexed | 2024-03-08T09:29:58Z |
| format | Article |
| id | doaj.art-6a6a922865444d5dbbc7563a7f5eaa0d |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-08T09:29:58Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-6a6a922865444d5dbbc7563a7f5eaa0d2024-01-31T04:43:45ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-01-011110.3389/fvets.2024.13617881361788Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herdsAlberto Gomez-Buendia0Alberto Gomez-Buendia1Julio Alvarez2Julio Alvarez3Javier Bezos4Javier Bezos5Jorge Mourelo6Javier Amado7Jose Luis Saez8Lucia de Juan9Lucia de Juan10Beatriz Romero11Beatriz Romero12VISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid, SpainDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainVISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid, SpainDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainVISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid, SpainDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainServicio de Sanidad Animal, Xunta de Galicia, Consellería de Medio Rural, Santiago de Compostela, SpainServicio de Laboratorio de Sanidad Animal y Vegetal, Dirección General de Ganadería y Sanidad Agraria, Consejería de Medio Rural y Política Agraria, Principado de Asturias, SpainSubdirección General de Sanidad e Higiene Animal y Trazabilidad, Dirección General de la Producción Agraria, Ministerio de Agricultura, Pesca y Alimentación, Madrid, SpainVISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid, SpainDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainVISAVET Health Surveillance Centre, Universidad Complutense de Madrid, Madrid, SpainDepartamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainNon-tuberculous mycobacteria (NTM) are considered a relevant cause of non-specific reactions to the most widely applied bovine tuberculosis (bTB) test, the intradermal tuberculin test. In order to establish which NTM species might act as a potential source of such diagnostic interference, a collection of 373 isolates obtained from skin test positive cows from 359 officially tuberculosis-free (OTF) herds, culled in the framework of the bTB eradication campaign in Spain, were identified at the species level through PCR and Sanger sequencing of the 16S rDNA, hsp65 and rpoB genes. Of the 308 isolates for which a reliable identification was achieved, 32 different mycobacterial species were identified, with certain species being most represented: among M. avium complex members (n = 142, 46.1%), M. avium subsp. hominissuis (98; 69.0%) was the most abundant followed by M. avium subsp. avium (33, 23.2%), and M. intracellulare (7, 4.9%). Among non-MAC members (n = 166, 53.9%), M. nonchromogenicum (85; 27.6%) and M. bourgelatii (11; 5.6%) were the predominant species. In addition, mixed results were obtained in 53 isolates presenting up to 30 different genotypes, which could be indicative of new mycobacterial species. Our results represent a first step toward characterizing the diversity of NTM species that could interfere with official diagnostic tests for bTB eradication in Spain.https://www.frontiersin.org/articles/10.3389/fvets.2024.1361788/fullcattlediagnosisofficially tuberculosis-free (OTF) herdsinterferencenon-tuberculous mycobacteriatuberculosis |
| spellingShingle | Alberto Gomez-Buendia Alberto Gomez-Buendia Julio Alvarez Julio Alvarez Javier Bezos Javier Bezos Jorge Mourelo Javier Amado Jose Luis Saez Lucia de Juan Lucia de Juan Beatriz Romero Beatriz Romero Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds Frontiers in Veterinary Science cattle diagnosis officially tuberculosis-free (OTF) herds interference non-tuberculous mycobacteria tuberculosis |
| title | Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds |
| title_full | Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds |
| title_fullStr | Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds |
| title_full_unstemmed | Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds |
| title_short | Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds |
| title_sort | non tuberculous mycobacteria occurrence in skin test cattle reactors from official tuberculosis free herds |
| topic | cattle diagnosis officially tuberculosis-free (OTF) herds interference non-tuberculous mycobacteria tuberculosis |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1361788/full |
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