Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i>
Antibiotics are well known drugs which, when present above certain concentrations, are able to inhibit the growth of certain bacteria. However, a growing body of evidence shows that even when present at lower doses (subMIC, for sub-minimal inhibitory concentration), unable to inhibit or affect micro...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/10/11/3227 |
| _version_ | 1797510795563630592 |
|---|---|
| author | André Carvalho Evelyne Krin Chloé Korlowski Didier Mazel Zeynep Baharoglu |
| author_facet | André Carvalho Evelyne Krin Chloé Korlowski Didier Mazel Zeynep Baharoglu |
| author_sort | André Carvalho |
| collection | DOAJ |
| description | Antibiotics are well known drugs which, when present above certain concentrations, are able to inhibit the growth of certain bacteria. However, a growing body of evidence shows that even when present at lower doses (subMIC, for sub-minimal inhibitory concentration), unable to inhibit or affect microbial growth, antibiotics work as signaling molecules, affect gene expression and trigger important bacterial stress responses. However, how subMIC antibiotic signaling interplays with other well-known signaling networks in bacteria (and the consequences of such interplay) is not well understood. In this work, through transcriptomic and genetic approaches, we have explored how quorum-sensing (QS) proficiency of <i>V. cholerae</i> affects this pathogen’s response to subMIC doses of the aminoglycoside tobramycin (TOB). We show that the transcriptomic signature of <i>V. cholerae</i> in response to subMIC TOB depends highly on the presence of QS master regulator HapR. In parallel, we show that subMIC doses of TOB are able to negatively interfere with the AI-2/LuxS QS network of <i>V. cholerae</i>, which seems critical for survival to aminoglycoside treatment and TOB-mediated induction of SOS response in this species. This interplay between QS and aminoglycosides suggests that targeting QS signaling may be a strategy to enhance aminoglycoside efficacy in <i>V. cholerae</i>. |
| first_indexed | 2024-03-10T05:36:20Z |
| format | Article |
| id | doaj.art-6a6a9ced4b4046c19aa8e0e33103fa17 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T05:36:20Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-6a6a9ced4b4046c19aa8e0e33103fa172023-11-22T22:53:09ZengMDPI AGCells2073-44092021-11-011011322710.3390/cells10113227Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i>André Carvalho0Evelyne Krin1Chloé Korlowski2Didier Mazel3Zeynep Baharoglu4Unité Plasticité du Génome Bactérien, Institut Pasteur, UMR3525 CNRS, 75015 Paris, FranceUnité Plasticité du Génome Bactérien, Institut Pasteur, UMR3525 CNRS, 75015 Paris, FranceUnité Plasticité du Génome Bactérien, Institut Pasteur, UMR3525 CNRS, 75015 Paris, FranceUnité Plasticité du Génome Bactérien, Institut Pasteur, UMR3525 CNRS, 75015 Paris, FranceUnité Plasticité du Génome Bactérien, Institut Pasteur, UMR3525 CNRS, 75015 Paris, FranceAntibiotics are well known drugs which, when present above certain concentrations, are able to inhibit the growth of certain bacteria. However, a growing body of evidence shows that even when present at lower doses (subMIC, for sub-minimal inhibitory concentration), unable to inhibit or affect microbial growth, antibiotics work as signaling molecules, affect gene expression and trigger important bacterial stress responses. However, how subMIC antibiotic signaling interplays with other well-known signaling networks in bacteria (and the consequences of such interplay) is not well understood. In this work, through transcriptomic and genetic approaches, we have explored how quorum-sensing (QS) proficiency of <i>V. cholerae</i> affects this pathogen’s response to subMIC doses of the aminoglycoside tobramycin (TOB). We show that the transcriptomic signature of <i>V. cholerae</i> in response to subMIC TOB depends highly on the presence of QS master regulator HapR. In parallel, we show that subMIC doses of TOB are able to negatively interfere with the AI-2/LuxS QS network of <i>V. cholerae</i>, which seems critical for survival to aminoglycoside treatment and TOB-mediated induction of SOS response in this species. This interplay between QS and aminoglycosides suggests that targeting QS signaling may be a strategy to enhance aminoglycoside efficacy in <i>V. cholerae</i>.https://www.mdpi.com/2073-4409/10/11/3227quorum sensingaminoglycosidesSOS response<i>Vibrio cholerae</i>bacterial signalingantibiotic tolerance |
| spellingShingle | André Carvalho Evelyne Krin Chloé Korlowski Didier Mazel Zeynep Baharoglu Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i> Cells quorum sensing aminoglycosides SOS response <i>Vibrio cholerae</i> bacterial signaling antibiotic tolerance |
| title | Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i> |
| title_full | Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i> |
| title_fullStr | Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i> |
| title_full_unstemmed | Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i> |
| title_short | Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i> |
| title_sort | interplay between sublethal aminoglycosides and quorum sensing consequences on survival in i v cholerae i |
| topic | quorum sensing aminoglycosides SOS response <i>Vibrio cholerae</i> bacterial signaling antibiotic tolerance |
| url | https://www.mdpi.com/2073-4409/10/11/3227 |
| work_keys_str_mv | AT andrecarvalho interplaybetweensublethalaminoglycosidesandquorumsensingconsequencesonsurvivalinivcholeraei AT evelynekrin interplaybetweensublethalaminoglycosidesandquorumsensingconsequencesonsurvivalinivcholeraei AT chloekorlowski interplaybetweensublethalaminoglycosidesandquorumsensingconsequencesonsurvivalinivcholeraei AT didiermazel interplaybetweensublethalaminoglycosidesandquorumsensingconsequencesonsurvivalinivcholeraei AT zeynepbaharoglu interplaybetweensublethalaminoglycosidesandquorumsensingconsequencesonsurvivalinivcholeraei |