Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction
The objectives of this study were to employ response surface methodology (RSM) to investigate and optimize the effect of ultrasound-assisted extraction (UAE) variables, temperature, time and amplitude on the yields of polysaccharides (fucose and total glucans) and antioxidant activities (ferric redu...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-07-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/16/8/257 |
| _version_ | 1811297746921979904 |
|---|---|
| author | Marco Garcia-Vaquero Gaurav Rajauria Brijesh Tiwari Torres Sweeney John O’Doherty |
| author_facet | Marco Garcia-Vaquero Gaurav Rajauria Brijesh Tiwari Torres Sweeney John O’Doherty |
| author_sort | Marco Garcia-Vaquero |
| collection | DOAJ |
| description | The objectives of this study were to employ response surface methodology (RSM) to investigate and optimize the effect of ultrasound-assisted extraction (UAE) variables, temperature, time and amplitude on the yields of polysaccharides (fucose and total glucans) and antioxidant activities (ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity (DPPH)) from Laminaria digitata, and to explore the suitability of applying the optimum UAE conditions for L. digitata to other brown macroalgae (L. hyperborea and Ascophyllum nodosum). The RSM with three-factor, four-level Box-Behnken Design (BBD) was used to study and optimize the extraction variables. A second order polynomial model fitted well to the experimental data with R2 values of 0.79, 0.66, 0.64, 0.73 for fucose, total glucans, FRAP and DPPH, respectively. The UAE parameters studied had a significant influence on the levels of fucose, FRAP and DPPH. The optimised UAE conditions (temperature = 76 °C, time = 10 min and amplitude = 100%) achieved yields of fucose (1060.7 ± 70.6 mg/100 g dried seaweed (ds)), total glucans (968.6 ± 13.3 mg/100 g ds), FRAP (8.7 ± 0.5 µM trolox/mg freeze-dried extract (fde)) and DPPH (11.0 ± 0.2%) in L. digitata. Polysaccharide rich extracts were also attained from L. hyperborea and A. nodosum with variable results when utilizing the optimum UAE conditions for L. digitata. |
| first_indexed | 2024-04-13T06:09:45Z |
| format | Article |
| id | doaj.art-6a6b1374abf24363b4e71c1c8c84b6ea |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T06:09:45Z |
| publishDate | 2018-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-6a6b1374abf24363b4e71c1c8c84b6ea2022-12-22T02:59:06ZengMDPI AGMarine Drugs1660-33972018-07-0116825710.3390/md16080257md16080257Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted ExtractionMarco Garcia-Vaquero0Gaurav Rajauria1Brijesh Tiwari2Torres Sweeney3John O’Doherty4School of Veterinary Medicine, Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, IrelandSchool of Agriculture and Food Science, University College Dublin, Lyons Research Farm, Celbridge, Co., Kildare W23 ENY2, IrelandDepartment of Food Biosciences, TEAGASC, Food Research Centre, Ashtown, Dublin 15, KN3K, IrelandSchool of Veterinary Medicine, Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, IrelandSchool of Agriculture and Food Science, University College Dublin, Lyons Research Farm, Celbridge, Co., Kildare W23 ENY2, IrelandThe objectives of this study were to employ response surface methodology (RSM) to investigate and optimize the effect of ultrasound-assisted extraction (UAE) variables, temperature, time and amplitude on the yields of polysaccharides (fucose and total glucans) and antioxidant activities (ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity (DPPH)) from Laminaria digitata, and to explore the suitability of applying the optimum UAE conditions for L. digitata to other brown macroalgae (L. hyperborea and Ascophyllum nodosum). The RSM with three-factor, four-level Box-Behnken Design (BBD) was used to study and optimize the extraction variables. A second order polynomial model fitted well to the experimental data with R2 values of 0.79, 0.66, 0.64, 0.73 for fucose, total glucans, FRAP and DPPH, respectively. The UAE parameters studied had a significant influence on the levels of fucose, FRAP and DPPH. The optimised UAE conditions (temperature = 76 °C, time = 10 min and amplitude = 100%) achieved yields of fucose (1060.7 ± 70.6 mg/100 g dried seaweed (ds)), total glucans (968.6 ± 13.3 mg/100 g ds), FRAP (8.7 ± 0.5 µM trolox/mg freeze-dried extract (fde)) and DPPH (11.0 ± 0.2%) in L. digitata. Polysaccharide rich extracts were also attained from L. hyperborea and A. nodosum with variable results when utilizing the optimum UAE conditions for L. digitata.http://www.mdpi.com/1660-3397/16/8/257seaweedfucoidanlaminarinFRAPDPPHultrasound-assisted extractioninnovative technologyoptimization |
| spellingShingle | Marco Garcia-Vaquero Gaurav Rajauria Brijesh Tiwari Torres Sweeney John O’Doherty Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction Marine Drugs seaweed fucoidan laminarin FRAP DPPH ultrasound-assisted extraction innovative technology optimization |
| title | Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction |
| title_full | Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction |
| title_fullStr | Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction |
| title_full_unstemmed | Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction |
| title_short | Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction |
| title_sort | extraction and yield optimisation of fucose glucans and associated antioxidant activities from laminaria digitata by applying response surface methodology to high intensity ultrasound assisted extraction |
| topic | seaweed fucoidan laminarin FRAP DPPH ultrasound-assisted extraction innovative technology optimization |
| url | http://www.mdpi.com/1660-3397/16/8/257 |
| work_keys_str_mv | AT marcogarciavaquero extractionandyieldoptimisationoffucoseglucansandassociatedantioxidantactivitiesfromlaminariadigitatabyapplyingresponsesurfacemethodologytohighintensityultrasoundassistedextraction AT gauravrajauria extractionandyieldoptimisationoffucoseglucansandassociatedantioxidantactivitiesfromlaminariadigitatabyapplyingresponsesurfacemethodologytohighintensityultrasoundassistedextraction AT brijeshtiwari extractionandyieldoptimisationoffucoseglucansandassociatedantioxidantactivitiesfromlaminariadigitatabyapplyingresponsesurfacemethodologytohighintensityultrasoundassistedextraction AT torressweeney extractionandyieldoptimisationoffucoseglucansandassociatedantioxidantactivitiesfromlaminariadigitatabyapplyingresponsesurfacemethodologytohighintensityultrasoundassistedextraction AT johnodoherty extractionandyieldoptimisationoffucoseglucansandassociatedantioxidantactivitiesfromlaminariadigitatabyapplyingresponsesurfacemethodologytohighintensityultrasoundassistedextraction |