Microbial Genes of Agricultural Importance in Maize Rhizosphere Unveiled Through Shotgun Metagenomics
Genes possessed by microbes in the rhizosphere influence the metabolic activities that occur in this zone. Although the maize rhizosphere has been reported to be a hotspot of genes, these genes remain under-investigated. Hence, this study aimed at identifying putative microbial genes with plant bene...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Spanish Journal of Soil Science |
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| Online Access: | https://www.frontierspartnerships.org/articles/10.3389/sjss.2022.10427/full |
| _version_ | 1797222378321739776 |
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| author | Oluwadara Pelumi Omotayo Ozede Nicholas Igiehon Olubukola Oluranti Babalola |
| author_facet | Oluwadara Pelumi Omotayo Ozede Nicholas Igiehon Olubukola Oluranti Babalola |
| author_sort | Oluwadara Pelumi Omotayo |
| collection | DOAJ |
| description | Genes possessed by microbes in the rhizosphere influence the metabolic activities that occur in this zone. Although the maize rhizosphere has been reported to be a hotspot of genes, these genes remain under-investigated. Hence, this study aimed at identifying putative microbial genes with plant beneficial functions in the underexplored maize rhizosphere microbiome using a shotgun metagenomics approach. Sampling was done at the flowering stage of the maize plants and both the rhizosphere and bulk soils were collected in triplicates. The metagenomes of the examined rhizosphere and bulk soils revealed genes involved in carbon fixation, nitrogen fixation, iron acquisition, heat and cold shock, phosphorus solubilization and utilization, sulfur cycling, and siderophore production. The beta diversity analysis showed significant variations (p < 0.05) in these genes across the examined rhizosphere and bulk soils which was further confirmed by the distinct separations between the samples as seen on the principal coordinate analysis (PCoA) plot. Contrarily, no significant difference was observed in diversity within the habitats (p = 0.99). The predominance of significant genes of agricultural importance such as the nifH, nifA, groES, and cspA in the rhizosphere metagenomes signifies that this region is endowed with beneficial organisms with potential for improving plant growth, mitigating stress, and reducing the effect of extreme temperatures, which can be optimized in developing biofertilizers. Therefore, the development of strategies that will help in cultivating these organisms, which are mostly unculturable, is encouraged. This would make them readily available for use as bio-inoculants and in other biotechnological applications. |
| first_indexed | 2024-04-24T13:20:23Z |
| format | Article |
| id | doaj.art-6a6d55675df74bfd92d5a80584cbb7fb |
| institution | Directory Open Access Journal |
| issn | 2253-6574 |
| language | English |
| last_indexed | 2024-04-24T13:20:23Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Spanish Journal of Soil Science |
| spelling | doaj.art-6a6d55675df74bfd92d5a80584cbb7fb2024-04-04T15:34:22ZengFrontiers Media S.A.Spanish Journal of Soil Science2253-65742022-07-011210.3389/sjss.2022.1042710427Microbial Genes of Agricultural Importance in Maize Rhizosphere Unveiled Through Shotgun MetagenomicsOluwadara Pelumi OmotayoOzede Nicholas IgiehonOlubukola Oluranti BabalolaGenes possessed by microbes in the rhizosphere influence the metabolic activities that occur in this zone. Although the maize rhizosphere has been reported to be a hotspot of genes, these genes remain under-investigated. Hence, this study aimed at identifying putative microbial genes with plant beneficial functions in the underexplored maize rhizosphere microbiome using a shotgun metagenomics approach. Sampling was done at the flowering stage of the maize plants and both the rhizosphere and bulk soils were collected in triplicates. The metagenomes of the examined rhizosphere and bulk soils revealed genes involved in carbon fixation, nitrogen fixation, iron acquisition, heat and cold shock, phosphorus solubilization and utilization, sulfur cycling, and siderophore production. The beta diversity analysis showed significant variations (p < 0.05) in these genes across the examined rhizosphere and bulk soils which was further confirmed by the distinct separations between the samples as seen on the principal coordinate analysis (PCoA) plot. Contrarily, no significant difference was observed in diversity within the habitats (p = 0.99). The predominance of significant genes of agricultural importance such as the nifH, nifA, groES, and cspA in the rhizosphere metagenomes signifies that this region is endowed with beneficial organisms with potential for improving plant growth, mitigating stress, and reducing the effect of extreme temperatures, which can be optimized in developing biofertilizers. Therefore, the development of strategies that will help in cultivating these organisms, which are mostly unculturable, is encouraged. This would make them readily available for use as bio-inoculants and in other biotechnological applications.https://www.frontierspartnerships.org/articles/10.3389/sjss.2022.10427/fullmaize rhizospheremicrobesmicrobial genessulfur cyclingiron acquisitionrhizosphere microbiome |
| spellingShingle | Oluwadara Pelumi Omotayo Ozede Nicholas Igiehon Olubukola Oluranti Babalola Microbial Genes of Agricultural Importance in Maize Rhizosphere Unveiled Through Shotgun Metagenomics Spanish Journal of Soil Science maize rhizosphere microbes microbial genes sulfur cycling iron acquisition rhizosphere microbiome |
| title | Microbial Genes of Agricultural Importance in Maize Rhizosphere Unveiled Through Shotgun Metagenomics |
| title_full | Microbial Genes of Agricultural Importance in Maize Rhizosphere Unveiled Through Shotgun Metagenomics |
| title_fullStr | Microbial Genes of Agricultural Importance in Maize Rhizosphere Unveiled Through Shotgun Metagenomics |
| title_full_unstemmed | Microbial Genes of Agricultural Importance in Maize Rhizosphere Unveiled Through Shotgun Metagenomics |
| title_short | Microbial Genes of Agricultural Importance in Maize Rhizosphere Unveiled Through Shotgun Metagenomics |
| title_sort | microbial genes of agricultural importance in maize rhizosphere unveiled through shotgun metagenomics |
| topic | maize rhizosphere microbes microbial genes sulfur cycling iron acquisition rhizosphere microbiome |
| url | https://www.frontierspartnerships.org/articles/10.3389/sjss.2022.10427/full |
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