Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes.
Alkannin is an active constituent from the root extract of Alkanna tinctoria of the Boraginaceae family and it may have utility as a heat shock protein 70 (HSP70) inducer in living organisms. Here, the effects of alkannin-induced HSP70 on ultraviolet (UV) B (40 mJ/cm(2))-induced apoptosis were inves...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23110127/?tool=EBI |
| _version_ | 1818400292664770560 |
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| author | Yoko Yoshihisa Mariame Ali Hassan Yukihiro Furusawa Yoshiaki Tabuchi Takashi Kondo Tadamichi Shimizu |
| author_facet | Yoko Yoshihisa Mariame Ali Hassan Yukihiro Furusawa Yoshiaki Tabuchi Takashi Kondo Tadamichi Shimizu |
| author_sort | Yoko Yoshihisa |
| collection | DOAJ |
| description | Alkannin is an active constituent from the root extract of Alkanna tinctoria of the Boraginaceae family and it may have utility as a heat shock protein 70 (HSP70) inducer in living organisms. Here, the effects of alkannin-induced HSP70 on ultraviolet (UV) B (40 mJ/cm(2))-induced apoptosis were investigated in human keratinocyte HaCaT cells. Pretreatment of cells with alkannin (1 µM) caused significant inhibition of UVB-induced apoptosis and caspase-3 cleavage. On the other hand, the addition of KNK437 (HSP70 inhibitor) reversed the action of alkannin increasing UVB-induced apoptosis in a dose-dependent manner. In addition, differences in gene expression associated with the suppression of UVB-induced apoptosis in the presence of alkannin were investigated using Gene Chip assay. Our results indicate that alkannin suppresses UVB-induced apoptosis through the induction of HSP70 in human keratinocytes, and therefore, we suggest the usefulness of using alkannin as an antiaging agent. |
| first_indexed | 2024-12-14T07:34:16Z |
| format | Article |
| id | doaj.art-6a6ed1781b33464183861b70e6b6ce1c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T07:34:16Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6a6ed1781b33464183861b70e6b6ce1c2022-12-21T23:11:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01710e4790310.1371/journal.pone.0047903Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes.Yoko YoshihisaMariame Ali HassanYukihiro FurusawaYoshiaki TabuchiTakashi KondoTadamichi ShimizuAlkannin is an active constituent from the root extract of Alkanna tinctoria of the Boraginaceae family and it may have utility as a heat shock protein 70 (HSP70) inducer in living organisms. Here, the effects of alkannin-induced HSP70 on ultraviolet (UV) B (40 mJ/cm(2))-induced apoptosis were investigated in human keratinocyte HaCaT cells. Pretreatment of cells with alkannin (1 µM) caused significant inhibition of UVB-induced apoptosis and caspase-3 cleavage. On the other hand, the addition of KNK437 (HSP70 inhibitor) reversed the action of alkannin increasing UVB-induced apoptosis in a dose-dependent manner. In addition, differences in gene expression associated with the suppression of UVB-induced apoptosis in the presence of alkannin were investigated using Gene Chip assay. Our results indicate that alkannin suppresses UVB-induced apoptosis through the induction of HSP70 in human keratinocytes, and therefore, we suggest the usefulness of using alkannin as an antiaging agent.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23110127/?tool=EBI |
| spellingShingle | Yoko Yoshihisa Mariame Ali Hassan Yukihiro Furusawa Yoshiaki Tabuchi Takashi Kondo Tadamichi Shimizu Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes. PLoS ONE |
| title | Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes. |
| title_full | Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes. |
| title_fullStr | Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes. |
| title_full_unstemmed | Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes. |
| title_short | Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes. |
| title_sort | alkannin hsp70 inducer protects against uvb induced apoptosis in human keratinocytes |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23110127/?tool=EBI |
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