Proteomic Analysis of patients with Epileptic Seizure and Psychogenic Non-epileptic Seizure; a Cross-Sectional Study
Introduction: There is an increasing interest in the use of different biomarkers to help distinguish psychogenic non-epileptic seizure (PNES) from epileptic seizures (ES). This study aimed to evaluate the patterns of differentially expressed serum proteins in ES and PNES cases. Methods: In this cro...
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| Format: | Article |
| Language: | English |
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Shahid Beheshti University of Medical Sciences
2020-03-01
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| Series: | Archives of Academic Emergency Medicine |
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| Online Access: | https://journals.sbmu.ac.ir/aaem/index.php/AAEM/article/view/593 |
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| author | Mohsen Parvareshi Hamrah Mostafa Rezaei Tavirani Monireh Movahedi Sanaz Ahmadi Karvigh |
| author_facet | Mohsen Parvareshi Hamrah Mostafa Rezaei Tavirani Monireh Movahedi Sanaz Ahmadi Karvigh |
| author_sort | Mohsen Parvareshi Hamrah |
| collection | DOAJ |
| description | Introduction: There is an increasing interest in the use of different biomarkers to help distinguish psychogenic non-epileptic seizure (PNES) from epileptic seizures (ES). This study aimed to evaluate the patterns of differentially expressed serum proteins in ES and PNES cases.
Methods: In this cross-sectional study, 4 patients with mesial temporal lobe epilepsy and 4 patients with PNES were selected from patients with history of recurrent seizures. Venous blood samples were obtained within 1 hour after seizure and serum proteomes as well as the extent of protein expression were analyzed.
Results:  361 proteins were identified; of these, expression of 197 proteins had altered. 110 (55.9%) proteins were down-regulated and 87 (44.1%) were up-regulated in the PNES samples compared to ES samples. The mean pI for deregulated proteins with 1.5 to 3 fold changes were 6.69 ± 1.68 in proteins with increasing expression in ES group and 5.88 ± 1.39 in proteins with increasing expression in PNES group (p = 0.008). The median and interquartile range (IQR) of molecular weight changes in proteins with 1.5 to 3 fold changes were 64 (22.0-86.0) in proteins whose expression had increased in ES group and 39.5 (26.0-61.5) in proteins whose expression had increased in PNES cases (p = 0.05).Â
Conclusion: Several spots with differential expression were observed by comparing patients with ES against the PNES groups, which could be potential biomarkers of the disease. Damage to the blood-brain barrier is the most important difference between the two groups, thus identifying total protein changes offers a key to the future of differentiating ES and PNES patients. |
| first_indexed | 2024-04-13T02:34:53Z |
| format | Article |
| id | doaj.art-6a743f4a037e4865abcfe0bd543afaa5 |
| institution | Directory Open Access Journal |
| issn | 2645-4904 |
| language | English |
| last_indexed | 2024-04-13T02:34:53Z |
| publishDate | 2020-03-01 |
| publisher | Shahid Beheshti University of Medical Sciences |
| record_format | Article |
| series | Archives of Academic Emergency Medicine |
| spelling | doaj.art-6a743f4a037e4865abcfe0bd543afaa52022-12-22T03:06:25ZengShahid Beheshti University of Medical SciencesArchives of Academic Emergency Medicine2645-49042020-03-018110.22037/aaem.v8i1.593379Proteomic Analysis of patients with Epileptic Seizure and Psychogenic Non-epileptic Seizure; a Cross-Sectional StudyMohsen Parvareshi Hamrah0Mostafa Rezaei Tavirani1Monireh Movahedi2Sanaz Ahmadi Karvigh3Department of Biochemistry, Faculty of Biological Science , North Tehran Branch, Islamic Azad University , Tehran , Iran.Proteomics Research Center, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Biochemistry, Islamic Azad University, Tehran North Branch, Tehran, IranDepartment of Neurology, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran.Introduction: There is an increasing interest in the use of different biomarkers to help distinguish psychogenic non-epileptic seizure (PNES) from epileptic seizures (ES). This study aimed to evaluate the patterns of differentially expressed serum proteins in ES and PNES cases. Methods: In this cross-sectional study, 4 patients with mesial temporal lobe epilepsy and 4 patients with PNES were selected from patients with history of recurrent seizures. Venous blood samples were obtained within 1 hour after seizure and serum proteomes as well as the extent of protein expression were analyzed. Results:  361 proteins were identified; of these, expression of 197 proteins had altered. 110 (55.9%) proteins were down-regulated and 87 (44.1%) were up-regulated in the PNES samples compared to ES samples. The mean pI for deregulated proteins with 1.5 to 3 fold changes were 6.69 ± 1.68 in proteins with increasing expression in ES group and 5.88 ± 1.39 in proteins with increasing expression in PNES group (p = 0.008). The median and interquartile range (IQR) of molecular weight changes in proteins with 1.5 to 3 fold changes were 64 (22.0-86.0) in proteins whose expression had increased in ES group and 39.5 (26.0-61.5) in proteins whose expression had increased in PNES cases (p = 0.05). Conclusion: Several spots with differential expression were observed by comparing patients with ES against the PNES groups, which could be potential biomarkers of the disease. Damage to the blood-brain barrier is the most important difference between the two groups, thus identifying total protein changes offers a key to the future of differentiating ES and PNES patients.https://journals.sbmu.ac.ir/aaem/index.php/AAEM/article/view/593Seizuresproteomicsbiomarkersdiagnosisdifferential |
| spellingShingle | Mohsen Parvareshi Hamrah Mostafa Rezaei Tavirani Monireh Movahedi Sanaz Ahmadi Karvigh Proteomic Analysis of patients with Epileptic Seizure and Psychogenic Non-epileptic Seizure; a Cross-Sectional Study Archives of Academic Emergency Medicine Seizures proteomics biomarkers diagnosis differential |
| title | Proteomic Analysis of patients with Epileptic Seizure and Psychogenic Non-epileptic Seizure; a Cross-Sectional Study |
| title_full | Proteomic Analysis of patients with Epileptic Seizure and Psychogenic Non-epileptic Seizure; a Cross-Sectional Study |
| title_fullStr | Proteomic Analysis of patients with Epileptic Seizure and Psychogenic Non-epileptic Seizure; a Cross-Sectional Study |
| title_full_unstemmed | Proteomic Analysis of patients with Epileptic Seizure and Psychogenic Non-epileptic Seizure; a Cross-Sectional Study |
| title_short | Proteomic Analysis of patients with Epileptic Seizure and Psychogenic Non-epileptic Seizure; a Cross-Sectional Study |
| title_sort | proteomic analysis of patients with epileptic seizure and psychogenic non epileptic seizure a cross sectional study |
| topic | Seizures proteomics biomarkers diagnosis differential |
| url | https://journals.sbmu.ac.ir/aaem/index.php/AAEM/article/view/593 |
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