Current Status and Perspectives on the Application of CRISPR/Cas9 Gene-Editing System to Develop a Low-Gluten, Non-Transgenic Wheat Variety
Wheat gluten contains epitopes that trigger celiac disease (CD). A life-long strict gluten-free diet is the only treatment accepted for CD. However, very low-gluten wheat may provide an alternative treatment to CD. Conventional plant breeding methods have not been sufficient to produce celiac-safe w...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/10/10/2351 |
| _version_ | 1827679535199420416 |
|---|---|
| author | Anil K. Verma Sayanti Mandal Aadhya Tiwari Chiara Monachesi Giulia N. Catassi Akash Srivastava Simona Gatti Elena Lionetti Carlo Catassi |
| author_facet | Anil K. Verma Sayanti Mandal Aadhya Tiwari Chiara Monachesi Giulia N. Catassi Akash Srivastava Simona Gatti Elena Lionetti Carlo Catassi |
| author_sort | Anil K. Verma |
| collection | DOAJ |
| description | Wheat gluten contains epitopes that trigger celiac disease (CD). A life-long strict gluten-free diet is the only treatment accepted for CD. However, very low-gluten wheat may provide an alternative treatment to CD. Conventional plant breeding methods have not been sufficient to produce celiac-safe wheat. RNA interference technology, to some extent, has succeeded in the development of safer wheat varieties. However, these varieties have multiple challenges in terms of their implementation. Clustered Regularly Interspaced Short Palindromic Repeats-associated nuclease 9 (CRISPR/Cas9) is a versatile gene-editing tool that has the ability to edit immunogenic gluten genes. So far, only a few studies have applied CRISPR/Cas9 to modify the wheat genome. In this article, we reviewed the published literature that applied CRISPR/Cas9 in wheat genome editing to investigate the current status of the CRISPR/Cas9 system to produce a low-immunogenic wheat variety. We found that in recent years, the CRISPR/Cas9 system has been continuously improved to edit the complex hexaploid wheat genome. Although some reduced immunogenic wheat varieties have been reported, CRISPR/Cas9 has still not been fully explored in terms of editing the wheat genome. We conclude that further studies are required to apply the CRISPR/Cas9 gene-editing system efficiently for the development of a celiac-safe wheat variety and to establish it as a “tool to celiac safe wheat”. |
| first_indexed | 2024-03-10T06:33:44Z |
| format | Article |
| id | doaj.art-6a7a417162e04d21ae5f0e79649cf104 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T06:33:44Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-6a7a417162e04d21ae5f0e79649cf1042023-11-22T18:14:41ZengMDPI AGFoods2304-81582021-10-011010235110.3390/foods10102351Current Status and Perspectives on the Application of CRISPR/Cas9 Gene-Editing System to Develop a Low-Gluten, Non-Transgenic Wheat VarietyAnil K. Verma0Sayanti Mandal1Aadhya Tiwari2Chiara Monachesi3Giulia N. Catassi4Akash Srivastava5Simona Gatti6Elena Lionetti7Carlo Catassi8Celiac Disease Research Laboratory, Polytechnic University of Marche, 60123 Ancona, ItalyInstitute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Ganeshkhind Road, Pune 411007, Maharashtra, IndiaDepartment of System Biology, MD Anderson Cancer Center, Houston, TX 77030, USACeliac Disease Research Laboratory, Polytechnic University of Marche, 60123 Ancona, ItalyDivision of Pediatrics, DISCO Department, Polytechnic University of Marche, 60123 Ancona, ItalyDepartment of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02906, USADivision of Pediatrics, DISCO Department, Polytechnic University of Marche, 60123 Ancona, ItalyDivision of Pediatrics, DISCO Department, Polytechnic University of Marche, 60123 Ancona, ItalyDivision of Pediatrics, DISCO Department, Polytechnic University of Marche, 60123 Ancona, ItalyWheat gluten contains epitopes that trigger celiac disease (CD). A life-long strict gluten-free diet is the only treatment accepted for CD. However, very low-gluten wheat may provide an alternative treatment to CD. Conventional plant breeding methods have not been sufficient to produce celiac-safe wheat. RNA interference technology, to some extent, has succeeded in the development of safer wheat varieties. However, these varieties have multiple challenges in terms of their implementation. Clustered Regularly Interspaced Short Palindromic Repeats-associated nuclease 9 (CRISPR/Cas9) is a versatile gene-editing tool that has the ability to edit immunogenic gluten genes. So far, only a few studies have applied CRISPR/Cas9 to modify the wheat genome. In this article, we reviewed the published literature that applied CRISPR/Cas9 in wheat genome editing to investigate the current status of the CRISPR/Cas9 system to produce a low-immunogenic wheat variety. We found that in recent years, the CRISPR/Cas9 system has been continuously improved to edit the complex hexaploid wheat genome. Although some reduced immunogenic wheat varieties have been reported, CRISPR/Cas9 has still not been fully explored in terms of editing the wheat genome. We conclude that further studies are required to apply the CRISPR/Cas9 gene-editing system efficiently for the development of a celiac-safe wheat variety and to establish it as a “tool to celiac safe wheat”.https://www.mdpi.com/2304-8158/10/10/2351celiac diseaseCRISPR/Cas9RNAiα-gliadinlow-glutennon-transgenic wheat |
| spellingShingle | Anil K. Verma Sayanti Mandal Aadhya Tiwari Chiara Monachesi Giulia N. Catassi Akash Srivastava Simona Gatti Elena Lionetti Carlo Catassi Current Status and Perspectives on the Application of CRISPR/Cas9 Gene-Editing System to Develop a Low-Gluten, Non-Transgenic Wheat Variety Foods celiac disease CRISPR/Cas9 RNAi α-gliadin low-gluten non-transgenic wheat |
| title | Current Status and Perspectives on the Application of CRISPR/Cas9 Gene-Editing System to Develop a Low-Gluten, Non-Transgenic Wheat Variety |
| title_full | Current Status and Perspectives on the Application of CRISPR/Cas9 Gene-Editing System to Develop a Low-Gluten, Non-Transgenic Wheat Variety |
| title_fullStr | Current Status and Perspectives on the Application of CRISPR/Cas9 Gene-Editing System to Develop a Low-Gluten, Non-Transgenic Wheat Variety |
| title_full_unstemmed | Current Status and Perspectives on the Application of CRISPR/Cas9 Gene-Editing System to Develop a Low-Gluten, Non-Transgenic Wheat Variety |
| title_short | Current Status and Perspectives on the Application of CRISPR/Cas9 Gene-Editing System to Develop a Low-Gluten, Non-Transgenic Wheat Variety |
| title_sort | current status and perspectives on the application of crispr cas9 gene editing system to develop a low gluten non transgenic wheat variety |
| topic | celiac disease CRISPR/Cas9 RNAi α-gliadin low-gluten non-transgenic wheat |
| url | https://www.mdpi.com/2304-8158/10/10/2351 |
| work_keys_str_mv | AT anilkverma currentstatusandperspectivesontheapplicationofcrisprcas9geneeditingsystemtodevelopalowglutennontransgenicwheatvariety AT sayantimandal currentstatusandperspectivesontheapplicationofcrisprcas9geneeditingsystemtodevelopalowglutennontransgenicwheatvariety AT aadhyatiwari currentstatusandperspectivesontheapplicationofcrisprcas9geneeditingsystemtodevelopalowglutennontransgenicwheatvariety AT chiaramonachesi currentstatusandperspectivesontheapplicationofcrisprcas9geneeditingsystemtodevelopalowglutennontransgenicwheatvariety AT giuliancatassi currentstatusandperspectivesontheapplicationofcrisprcas9geneeditingsystemtodevelopalowglutennontransgenicwheatvariety AT akashsrivastava currentstatusandperspectivesontheapplicationofcrisprcas9geneeditingsystemtodevelopalowglutennontransgenicwheatvariety AT simonagatti currentstatusandperspectivesontheapplicationofcrisprcas9geneeditingsystemtodevelopalowglutennontransgenicwheatvariety AT elenalionetti currentstatusandperspectivesontheapplicationofcrisprcas9geneeditingsystemtodevelopalowglutennontransgenicwheatvariety AT carlocatassi currentstatusandperspectivesontheapplicationofcrisprcas9geneeditingsystemtodevelopalowglutennontransgenicwheatvariety |