Anti-inflammatory effects of yeast-derived vacuoles on LPS-induced murine macrophage activation
ABSTRACT Saccharomyces cerevisiae is a single-celled fungal microorganism. S. cerevisiae-derived vacuoles are closely related to mammalian lysosomes, which play a role in the degradation of macromolecules by various hydrolytic enzymes. This study evaluated the anti-inflammatory efficacy of S. cerevi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-10-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01466-23 |
| _version_ | 1797658221699137536 |
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| author | Su-Min Lee Yang-Hoon Kim Jiho Min |
| author_facet | Su-Min Lee Yang-Hoon Kim Jiho Min |
| author_sort | Su-Min Lee |
| collection | DOAJ |
| description | ABSTRACT Saccharomyces cerevisiae is a single-celled fungal microorganism. S. cerevisiae-derived vacuoles are closely related to mammalian lysosomes, which play a role in the degradation of macromolecules by various hydrolytic enzymes. This study evaluated the anti-inflammatory efficacy of S. cerevisiae-vacuoles by inhibiting inflammatory mediators induced by lipopolysaccharide (LPS). The results showed that treatment with 5, 10, and 20 µg/mL of S. cerevisiae-derived vacuoles almost completely inhibited the LPS-induced expression of iNOS protein and mRNA. Moreover, vacuoles significantly reduced the mRNA expression of tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) in LPS-stimulated macrophages compared to the control cells. The immunofluorescence analysis confirmed that S. cerevisiae-derived vacuoles inhibited the translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in LPS-stimulated cells. Taken together, the treatment with S. cerevisiae-derived vacuoles alone activated macrophages, but LPS-activated macrophages modulated pro-inflammatory mediators by downregulating the NF-κB pathway. These results suggest that S. cerevisiae-derived vacuoles may have therapeutic potential in the treatment of inflammatory diseases. In conclusion, our study provides new insights into the immunomodulatory effects of S. cerevisiae-derived vacuoles and their potential as a novel anti-inflammatory agent. IMPORTANCE This study investigates the potential of using vacuoles derived from the yeast Saccharomyces cerevisiae as a new anti-inflammatory therapy. Inflammation is a natural response of the immune system to invading pathogens, but when it is dysregulated, it can lead to chronic diseases. The researchers found that treating macrophages with vacuoles significantly reduced the production of pro-inflammatory cytokines and iNOS, markers of inflammation when they were stimulated with lipopolysaccharide. The study also showed that vacuoles inhibited the NF-κB signaling pathway, which is involved in the induction of pro-inflammatory cytokines in macrophages. These findings suggest that S. cerevisiae-derived vacuoles may have potential as a new therapeutic agent for regulating the inflammatory response in various diseases. Further studies are needed to evaluate the efficacy and safety of vacuoles in vivo and to elucidate the underlying mechanisms of their anti-inflammatory effects. |
| first_indexed | 2024-03-11T17:56:07Z |
| format | Article |
| id | doaj.art-6a7bdeb21014485caefc52a8e34c3220 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-11T17:56:07Z |
| publishDate | 2023-10-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-6a7bdeb21014485caefc52a8e34c32202023-10-17T13:04:35ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-10-0111510.1128/spectrum.01466-23Anti-inflammatory effects of yeast-derived vacuoles on LPS-induced murine macrophage activationSu-Min Lee0Yang-Hoon Kim1Jiho Min2Graduate School of Semiconductor and Chemical Engineering, Jeonbuk National University , Jeonbuk, South KoreaSchool of Biological Sciences, Chungbuk National University , Cheongju, South KoreaGraduate School of Semiconductor and Chemical Engineering, Jeonbuk National University , Jeonbuk, South KoreaABSTRACT Saccharomyces cerevisiae is a single-celled fungal microorganism. S. cerevisiae-derived vacuoles are closely related to mammalian lysosomes, which play a role in the degradation of macromolecules by various hydrolytic enzymes. This study evaluated the anti-inflammatory efficacy of S. cerevisiae-vacuoles by inhibiting inflammatory mediators induced by lipopolysaccharide (LPS). The results showed that treatment with 5, 10, and 20 µg/mL of S. cerevisiae-derived vacuoles almost completely inhibited the LPS-induced expression of iNOS protein and mRNA. Moreover, vacuoles significantly reduced the mRNA expression of tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) in LPS-stimulated macrophages compared to the control cells. The immunofluorescence analysis confirmed that S. cerevisiae-derived vacuoles inhibited the translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in LPS-stimulated cells. Taken together, the treatment with S. cerevisiae-derived vacuoles alone activated macrophages, but LPS-activated macrophages modulated pro-inflammatory mediators by downregulating the NF-κB pathway. These results suggest that S. cerevisiae-derived vacuoles may have therapeutic potential in the treatment of inflammatory diseases. In conclusion, our study provides new insights into the immunomodulatory effects of S. cerevisiae-derived vacuoles and their potential as a novel anti-inflammatory agent. IMPORTANCE This study investigates the potential of using vacuoles derived from the yeast Saccharomyces cerevisiae as a new anti-inflammatory therapy. Inflammation is a natural response of the immune system to invading pathogens, but when it is dysregulated, it can lead to chronic diseases. The researchers found that treating macrophages with vacuoles significantly reduced the production of pro-inflammatory cytokines and iNOS, markers of inflammation when they were stimulated with lipopolysaccharide. The study also showed that vacuoles inhibited the NF-κB signaling pathway, which is involved in the induction of pro-inflammatory cytokines in macrophages. These findings suggest that S. cerevisiae-derived vacuoles may have potential as a new therapeutic agent for regulating the inflammatory response in various diseases. Further studies are needed to evaluate the efficacy and safety of vacuoles in vivo and to elucidate the underlying mechanisms of their anti-inflammatory effects.https://journals.asm.org/doi/10.1128/spectrum.01466-23S. cerevisiae-derived vacuolemacrophagelipopolysaccharideanti-inflammatory |
| spellingShingle | Su-Min Lee Yang-Hoon Kim Jiho Min Anti-inflammatory effects of yeast-derived vacuoles on LPS-induced murine macrophage activation Microbiology Spectrum S. cerevisiae-derived vacuole macrophage lipopolysaccharide anti-inflammatory |
| title | Anti-inflammatory effects of yeast-derived vacuoles on LPS-induced murine macrophage activation |
| title_full | Anti-inflammatory effects of yeast-derived vacuoles on LPS-induced murine macrophage activation |
| title_fullStr | Anti-inflammatory effects of yeast-derived vacuoles on LPS-induced murine macrophage activation |
| title_full_unstemmed | Anti-inflammatory effects of yeast-derived vacuoles on LPS-induced murine macrophage activation |
| title_short | Anti-inflammatory effects of yeast-derived vacuoles on LPS-induced murine macrophage activation |
| title_sort | anti inflammatory effects of yeast derived vacuoles on lps induced murine macrophage activation |
| topic | S. cerevisiae-derived vacuole macrophage lipopolysaccharide anti-inflammatory |
| url | https://journals.asm.org/doi/10.1128/spectrum.01466-23 |
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