Quality Evaluation of Crude and Salt-Processed Cuscutae Semen through Qualitative and Quantitative Analysis of Multiple Components Using HPLC Combined with Chemometrics
Cuscutae Semen (CS; Chinese common name “Tusizi”) is one of the most common traditional herbal medicines used to treat liver and kidney diseases in China. Although it is most commonly used as a processed product, little progress has been made on the quality control of CS and salt-processed Cuscutae...
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MDPI AG
2022-08-01
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| author | Yun Shi Chang-Li Xu Yu-Jie Zhu Yi-Wen Tian Xiao Liu Xun Gao Kun-Ming Qin Wei-Dong Li |
| author_facet | Yun Shi Chang-Li Xu Yu-Jie Zhu Yi-Wen Tian Xiao Liu Xun Gao Kun-Ming Qin Wei-Dong Li |
| author_sort | Yun Shi |
| collection | DOAJ |
| description | Cuscutae Semen (CS; Chinese common name “Tusizi”) is one of the most common traditional herbal medicines used to treat liver and kidney diseases in China. Although it is most commonly used as a processed product, little progress has been made on the quality control of CS and salt-processed Cuscutae Semen (PCS). The purpose of this study was to establish a comprehensive strategy integrating chromatographic analysis and chemometric methods for quality evaluation and discrimination of CS and PCS. An accurate and reliable HPLC method was established for the simultaneous quantification of 12 analyte compounds in CS and PCS. The results showed that this method exhibited desirable sensitivity, precision, stability, and repeatability. Multivariate chemometric methods were applied to analyze the obtained HPLC fingerprints, including hierarchical cluster analysis (HCA), principle component analysis (PCA), and partial least squares discriminant analysis (PLS-DA). The results indicated that CS and PCS samples showed a clear classification of the two groups, and three chemical markers with great contributions to the differentiation were screened out. Collectively, the chemometrics combined with the quantitative analysis based on HPLC fingerprint results indicated that salt processing may change the contents and types of components in Cuscutae Semen. |
| first_indexed | 2024-03-09T22:31:54Z |
| format | Article |
| id | doaj.art-6a7c57fa10204713951a1c67063ea1cd |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-09T22:31:54Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-6a7c57fa10204713951a1c67063ea1cd2023-11-23T18:54:27ZengMDPI AGSeparations2297-87392022-08-019923110.3390/separations9090231Quality Evaluation of Crude and Salt-Processed Cuscutae Semen through Qualitative and Quantitative Analysis of Multiple Components Using HPLC Combined with ChemometricsYun Shi0Chang-Li Xu1Yu-Jie Zhu2Yi-Wen Tian3Xiao Liu4Xun Gao5Kun-Ming Qin6Wei-Dong Li7Key Laboratory of State Administration of Traditional Chinese Medicine for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaPharmacy School, Jiangsu Ocean University, Lianyungang 222005, ChinaPharmacy School, Jiangsu Ocean University, Lianyungang 222005, ChinaPharmacy School, Jiangsu Ocean University, Lianyungang 222005, ChinaKey Laboratory of State Administration of Traditional Chinese Medicine for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaPharmacy School, Jiangsu Ocean University, Lianyungang 222005, ChinaPharmacy School, Jiangsu Ocean University, Lianyungang 222005, ChinaKey Laboratory of State Administration of Traditional Chinese Medicine for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaCuscutae Semen (CS; Chinese common name “Tusizi”) is one of the most common traditional herbal medicines used to treat liver and kidney diseases in China. Although it is most commonly used as a processed product, little progress has been made on the quality control of CS and salt-processed Cuscutae Semen (PCS). The purpose of this study was to establish a comprehensive strategy integrating chromatographic analysis and chemometric methods for quality evaluation and discrimination of CS and PCS. An accurate and reliable HPLC method was established for the simultaneous quantification of 12 analyte compounds in CS and PCS. The results showed that this method exhibited desirable sensitivity, precision, stability, and repeatability. Multivariate chemometric methods were applied to analyze the obtained HPLC fingerprints, including hierarchical cluster analysis (HCA), principle component analysis (PCA), and partial least squares discriminant analysis (PLS-DA). The results indicated that CS and PCS samples showed a clear classification of the two groups, and three chemical markers with great contributions to the differentiation were screened out. Collectively, the chemometrics combined with the quantitative analysis based on HPLC fingerprint results indicated that salt processing may change the contents and types of components in Cuscutae Semen.https://www.mdpi.com/2297-8739/9/9/231Cuscutae Semenmultivariate statistical analysishigh-performance liquid chromatographysalt-processed |
| spellingShingle | Yun Shi Chang-Li Xu Yu-Jie Zhu Yi-Wen Tian Xiao Liu Xun Gao Kun-Ming Qin Wei-Dong Li Quality Evaluation of Crude and Salt-Processed Cuscutae Semen through Qualitative and Quantitative Analysis of Multiple Components Using HPLC Combined with Chemometrics Separations Cuscutae Semen multivariate statistical analysis high-performance liquid chromatography salt-processed |
| title | Quality Evaluation of Crude and Salt-Processed Cuscutae Semen through Qualitative and Quantitative Analysis of Multiple Components Using HPLC Combined with Chemometrics |
| title_full | Quality Evaluation of Crude and Salt-Processed Cuscutae Semen through Qualitative and Quantitative Analysis of Multiple Components Using HPLC Combined with Chemometrics |
| title_fullStr | Quality Evaluation of Crude and Salt-Processed Cuscutae Semen through Qualitative and Quantitative Analysis of Multiple Components Using HPLC Combined with Chemometrics |
| title_full_unstemmed | Quality Evaluation of Crude and Salt-Processed Cuscutae Semen through Qualitative and Quantitative Analysis of Multiple Components Using HPLC Combined with Chemometrics |
| title_short | Quality Evaluation of Crude and Salt-Processed Cuscutae Semen through Qualitative and Quantitative Analysis of Multiple Components Using HPLC Combined with Chemometrics |
| title_sort | quality evaluation of crude and salt processed cuscutae semen through qualitative and quantitative analysis of multiple components using hplc combined with chemometrics |
| topic | Cuscutae Semen multivariate statistical analysis high-performance liquid chromatography salt-processed |
| url | https://www.mdpi.com/2297-8739/9/9/231 |
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