Distinct metabolic profile of primary focal segmental glomerulosclerosis revealed by NMR-based metabolomics.
BACKGROUND: Primary focal segmental glomerulosclerosis (FSGS) is pathological entity which is characterized by idiopathic steroid-resistant nephrotic syndrome (SRNS) and progression to end-stage renal disease (ESRD) in the majority of affected individuals. Currently, there is no practical noninvasiv...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3823857?pdf=render |
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| author | Xu Hao Xia Liu Weiming Wang Hong Ren Jingyuan Xie Pingyan Shen Donghai Lin Nan Chen |
| author_facet | Xu Hao Xia Liu Weiming Wang Hong Ren Jingyuan Xie Pingyan Shen Donghai Lin Nan Chen |
| author_sort | Xu Hao |
| collection | DOAJ |
| description | BACKGROUND: Primary focal segmental glomerulosclerosis (FSGS) is pathological entity which is characterized by idiopathic steroid-resistant nephrotic syndrome (SRNS) and progression to end-stage renal disease (ESRD) in the majority of affected individuals. Currently, there is no practical noninvasive technique to predict different pathological types of glomerulopathies. In this study, the role of urinary metabolomics in the diagnosis and pathogenesis of FSGS was investigated. METHODS: NMR-based metabolomics was applied for the urinary metabolic profile in the patients with FSGS (n = 25), membranous nephropathy (MN, n = 24), minimal change disease (MCD, n = 14) and IgA nephropathy (IgAN, n = 26), and healthy controls (CON, n = 35). The acquired data were analyzed using principal component analysis (PCA) followed by orthogonal projections to latent structure discriminant analysis (OPLS-DA). Model validity was verified using permutation tests. RESULTS: FSGS patients were clearly distinguished from healthy controls and other three types of glomerulopathies with good sensitivity and specificity based on their global urinary metabolic profiles. In FSGS patients, urinary levels of glucose, dimethylamine and trimethylamine increased compared with healthy controls, while pyruvate, valine, hippurate, isoleucine, phenylacetylglycine, citrate, tyrosine, 3-methylhistidine and β-hydroxyisovalerate decreased. Additionally, FSGS patients had lower urine N-methylnicotinamide levels compared with other glomerulopathies. CONCLUSIONS: NMR-based metabonomic approach is amenable for the noninvasive diagnosis and differential diagnosis of FSGS as well as other glomerulopathies, and it could indicate the possible mechanisms of primary FSGS. |
| first_indexed | 2024-04-12T18:24:24Z |
| format | Article |
| id | doaj.art-6a8138f8ba874a999b9ced58f8194e6b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T18:24:24Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-6a8138f8ba874a999b9ced58f8194e6b2022-12-22T03:21:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7853110.1371/journal.pone.0078531Distinct metabolic profile of primary focal segmental glomerulosclerosis revealed by NMR-based metabolomics.Xu HaoXia LiuWeiming WangHong RenJingyuan XiePingyan ShenDonghai LinNan ChenBACKGROUND: Primary focal segmental glomerulosclerosis (FSGS) is pathological entity which is characterized by idiopathic steroid-resistant nephrotic syndrome (SRNS) and progression to end-stage renal disease (ESRD) in the majority of affected individuals. Currently, there is no practical noninvasive technique to predict different pathological types of glomerulopathies. In this study, the role of urinary metabolomics in the diagnosis and pathogenesis of FSGS was investigated. METHODS: NMR-based metabolomics was applied for the urinary metabolic profile in the patients with FSGS (n = 25), membranous nephropathy (MN, n = 24), minimal change disease (MCD, n = 14) and IgA nephropathy (IgAN, n = 26), and healthy controls (CON, n = 35). The acquired data were analyzed using principal component analysis (PCA) followed by orthogonal projections to latent structure discriminant analysis (OPLS-DA). Model validity was verified using permutation tests. RESULTS: FSGS patients were clearly distinguished from healthy controls and other three types of glomerulopathies with good sensitivity and specificity based on their global urinary metabolic profiles. In FSGS patients, urinary levels of glucose, dimethylamine and trimethylamine increased compared with healthy controls, while pyruvate, valine, hippurate, isoleucine, phenylacetylglycine, citrate, tyrosine, 3-methylhistidine and β-hydroxyisovalerate decreased. Additionally, FSGS patients had lower urine N-methylnicotinamide levels compared with other glomerulopathies. CONCLUSIONS: NMR-based metabonomic approach is amenable for the noninvasive diagnosis and differential diagnosis of FSGS as well as other glomerulopathies, and it could indicate the possible mechanisms of primary FSGS.http://europepmc.org/articles/PMC3823857?pdf=render |
| spellingShingle | Xu Hao Xia Liu Weiming Wang Hong Ren Jingyuan Xie Pingyan Shen Donghai Lin Nan Chen Distinct metabolic profile of primary focal segmental glomerulosclerosis revealed by NMR-based metabolomics. PLoS ONE |
| title | Distinct metabolic profile of primary focal segmental glomerulosclerosis revealed by NMR-based metabolomics. |
| title_full | Distinct metabolic profile of primary focal segmental glomerulosclerosis revealed by NMR-based metabolomics. |
| title_fullStr | Distinct metabolic profile of primary focal segmental glomerulosclerosis revealed by NMR-based metabolomics. |
| title_full_unstemmed | Distinct metabolic profile of primary focal segmental glomerulosclerosis revealed by NMR-based metabolomics. |
| title_short | Distinct metabolic profile of primary focal segmental glomerulosclerosis revealed by NMR-based metabolomics. |
| title_sort | distinct metabolic profile of primary focal segmental glomerulosclerosis revealed by nmr based metabolomics |
| url | http://europepmc.org/articles/PMC3823857?pdf=render |
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