The serodiagnositic value of Chlamydia trachomatis antigens in antibody detection using luciferase immunosorbent assay
IntroductionAmong the different antigens used in the detection of anti-Chlamydia trachomatis antibodies, significant differences in sensitivity and specificity have been observed. Further evaluation of C. trachomatis antigens in antibody detection is urgently needed for the development and applicati...
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Public Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpubh.2024.1333559/full |
| _version_ | 1797294207775277056 |
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| author | Yulian Pang Jingwei Shui Jingwei Shui Changchang Li Yongzhi Li Hongliang Chen Shixing Tang |
| author_facet | Yulian Pang Jingwei Shui Jingwei Shui Changchang Li Yongzhi Li Hongliang Chen Shixing Tang |
| author_sort | Yulian Pang |
| collection | DOAJ |
| description | IntroductionAmong the different antigens used in the detection of anti-Chlamydia trachomatis antibodies, significant differences in sensitivity and specificity have been observed. Further evaluation of C. trachomatis antigens in antibody detection is urgently needed for the development and application of C. trachomatis serologic assays.MethodsChlamydia trachomatis antigens Pgp3, TmeA, InaC, and HSP60 were selected and used in luciferase immunosorbent assay (LISA). The detection results obtained from well-defined C. trachomatis positive and negative samples were compared with the commercial C. trachomatis ELISA (Mikrogen) for performance evaluation.ResultsPgp3, TmeA, InaC, and HSP60-based LISA showed sensitivity of 92.8, 88.8, 90.4, and 94.4%, and specificity of 99.2, 99.2, 99.2, and 92%, respectively. ROC analysis indicated that Pgp3-based LISA showed similar performance to Mikrogen ELISA (AUC 0.986 vs. 0.993, p = 0.207). Furthermore, four C. trachomatis antigens achieved strong diagnostic efficiency, i.e., positive likelihood ratios [+LR] ≥ 10 in C. trachomatis-infected women and negative likelihood ratios [−LR] ≤ 0.1 in C. trachomatis negative low exposure risk children, but only Pgp3 and TmeA showed strong diagnostic value in general adults. In addition, Pgp3, TmeA, and InaC, but not HSP60, achieved high performance, i.e., both positive predictive value (PPV) and negative predictive value (NPV) ≥ 90.9%, and showed no significant cross-reactivity with anti-Chlamydiapneumoniae.ConclusionThree C. trachomatis species-specific antigens Pgp3, TmeA, and InaC show superior performance in the detection of anti-C. trachomatis antibody, indicating the potential to be used in developing C. trachomatis serologic tests. |
| first_indexed | 2024-03-07T21:26:54Z |
| format | Article |
| id | doaj.art-6a8443d8f18b4638a186635587b4b4e5 |
| institution | Directory Open Access Journal |
| issn | 2296-2565 |
| language | English |
| last_indexed | 2024-03-07T21:26:54Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj.art-6a8443d8f18b4638a186635587b4b4e52024-02-27T04:24:08ZengFrontiers Media S.A.Frontiers in Public Health2296-25652024-02-011210.3389/fpubh.2024.13335591333559The serodiagnositic value of Chlamydia trachomatis antigens in antibody detection using luciferase immunosorbent assayYulian Pang0Jingwei Shui1Jingwei Shui2Changchang Li3Yongzhi Li4Hongliang Chen5Shixing Tang6Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Emergency, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaDermatology Hospital of Southern Medical University, Guangzhou, ChinaDepartment of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, Guangdong, ChinaChenzhou No.1 People's Hospital, Chenzhou, ChinaDepartment of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, Guangdong, ChinaIntroductionAmong the different antigens used in the detection of anti-Chlamydia trachomatis antibodies, significant differences in sensitivity and specificity have been observed. Further evaluation of C. trachomatis antigens in antibody detection is urgently needed for the development and application of C. trachomatis serologic assays.MethodsChlamydia trachomatis antigens Pgp3, TmeA, InaC, and HSP60 were selected and used in luciferase immunosorbent assay (LISA). The detection results obtained from well-defined C. trachomatis positive and negative samples were compared with the commercial C. trachomatis ELISA (Mikrogen) for performance evaluation.ResultsPgp3, TmeA, InaC, and HSP60-based LISA showed sensitivity of 92.8, 88.8, 90.4, and 94.4%, and specificity of 99.2, 99.2, 99.2, and 92%, respectively. ROC analysis indicated that Pgp3-based LISA showed similar performance to Mikrogen ELISA (AUC 0.986 vs. 0.993, p = 0.207). Furthermore, four C. trachomatis antigens achieved strong diagnostic efficiency, i.e., positive likelihood ratios [+LR] ≥ 10 in C. trachomatis-infected women and negative likelihood ratios [−LR] ≤ 0.1 in C. trachomatis negative low exposure risk children, but only Pgp3 and TmeA showed strong diagnostic value in general adults. In addition, Pgp3, TmeA, and InaC, but not HSP60, achieved high performance, i.e., both positive predictive value (PPV) and negative predictive value (NPV) ≥ 90.9%, and showed no significant cross-reactivity with anti-Chlamydiapneumoniae.ConclusionThree C. trachomatis species-specific antigens Pgp3, TmeA, and InaC show superior performance in the detection of anti-C. trachomatis antibody, indicating the potential to be used in developing C. trachomatis serologic tests.https://www.frontiersin.org/articles/10.3389/fpubh.2024.1333559/fullChlamydia trachomatisluciferase immunosorbent assay (LISA)plasmid-encoded protein 3 (Pgp3)translocated membrane-associated effector A (TmeA)inclusion membrane protein for actin assembly (InaC)heat shock protein 60 (HSP60) |
| spellingShingle | Yulian Pang Jingwei Shui Jingwei Shui Changchang Li Yongzhi Li Hongliang Chen Shixing Tang The serodiagnositic value of Chlamydia trachomatis antigens in antibody detection using luciferase immunosorbent assay Frontiers in Public Health Chlamydia trachomatis luciferase immunosorbent assay (LISA) plasmid-encoded protein 3 (Pgp3) translocated membrane-associated effector A (TmeA) inclusion membrane protein for actin assembly (InaC) heat shock protein 60 (HSP60) |
| title | The serodiagnositic value of Chlamydia trachomatis antigens in antibody detection using luciferase immunosorbent assay |
| title_full | The serodiagnositic value of Chlamydia trachomatis antigens in antibody detection using luciferase immunosorbent assay |
| title_fullStr | The serodiagnositic value of Chlamydia trachomatis antigens in antibody detection using luciferase immunosorbent assay |
| title_full_unstemmed | The serodiagnositic value of Chlamydia trachomatis antigens in antibody detection using luciferase immunosorbent assay |
| title_short | The serodiagnositic value of Chlamydia trachomatis antigens in antibody detection using luciferase immunosorbent assay |
| title_sort | serodiagnositic value of chlamydia trachomatis antigens in antibody detection using luciferase immunosorbent assay |
| topic | Chlamydia trachomatis luciferase immunosorbent assay (LISA) plasmid-encoded protein 3 (Pgp3) translocated membrane-associated effector A (TmeA) inclusion membrane protein for actin assembly (InaC) heat shock protein 60 (HSP60) |
| url | https://www.frontiersin.org/articles/10.3389/fpubh.2024.1333559/full |
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