In situ modelling of biofilm formation in a hydrothermal spring cave
Abstract Attachment of microorganisms to natural or artificial surfaces and the development of biofilms are complex processes which can be influenced by several factors. Nevertheless, our knowledge on biofilm formation in karstic environment is quite incomplete. The present study aimed to examine bi...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-78759-4 |
| _version_ | 1819200230175801344 |
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| author | Dóra Anda Attila Szabó Petra Kovács-Bodor Judit Makk Tamás Felföldi Éva Ács Judit Mádl-Szőnyi Andrea K. Borsodi |
| author_facet | Dóra Anda Attila Szabó Petra Kovács-Bodor Judit Makk Tamás Felföldi Éva Ács Judit Mádl-Szőnyi Andrea K. Borsodi |
| author_sort | Dóra Anda |
| collection | DOAJ |
| description | Abstract Attachment of microorganisms to natural or artificial surfaces and the development of biofilms are complex processes which can be influenced by several factors. Nevertheless, our knowledge on biofilm formation in karstic environment is quite incomplete. The present study aimed to examine biofilm development for a year under controlled conditions in quasi-stagnant water of a hydrothermal spring cave located in the Buda Thermal Karst System (Hungary). Using a model system, we investigated how the structure of the biofilm is formed from the water and also how the growth rate of biofilm development takes place in this environment. Besides scanning electron microscopy, next-generation DNA sequencing was used to reveal the characteristic taxa and major shifts in the composition of the bacterial communities. Dynamic temporal changes were observed in the structure of bacterial communities. Bacterial richness and diversity increased during the biofilm formation, and 9–12 weeks were needed for the maturation. Increasing EPS production was also observed from the 9–12 weeks. The biofilm was different from the water that filled the cave pool, in terms of the taxonomic composition and metabolic potential of microorganisms. In these karstic environments, the formation of mature biofilm appears to take place relatively quickly, in a few months. |
| first_indexed | 2024-12-23T03:28:55Z |
| format | Article |
| id | doaj.art-6a8a73e80c68428ea1f9bd82e05e0a56 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T03:28:55Z |
| publishDate | 2020-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6a8a73e80c68428ea1f9bd82e05e0a562022-12-21T18:01:45ZengNature PortfolioScientific Reports2045-23222020-12-011011910.1038/s41598-020-78759-4In situ modelling of biofilm formation in a hydrothermal spring caveDóra Anda0Attila Szabó1Petra Kovács-Bodor2Judit Makk3Tamás Felföldi4Éva Ács5Judit Mádl-Szőnyi6Andrea K. Borsodi7Department of Microbiology, ELTE Eötvös Loránd UniversityDepartment of Microbiology, ELTE Eötvös Loránd UniversityDepartment of Geology, ELTE Eötvös Loránd UniversityDepartment of Microbiology, ELTE Eötvös Loránd UniversityDepartment of Microbiology, ELTE Eötvös Loránd UniversityDanube Research Institute, Centre for Ecological ResearchDepartment of Geology, ELTE Eötvös Loránd UniversityDepartment of Microbiology, ELTE Eötvös Loránd UniversityAbstract Attachment of microorganisms to natural or artificial surfaces and the development of biofilms are complex processes which can be influenced by several factors. Nevertheless, our knowledge on biofilm formation in karstic environment is quite incomplete. The present study aimed to examine biofilm development for a year under controlled conditions in quasi-stagnant water of a hydrothermal spring cave located in the Buda Thermal Karst System (Hungary). Using a model system, we investigated how the structure of the biofilm is formed from the water and also how the growth rate of biofilm development takes place in this environment. Besides scanning electron microscopy, next-generation DNA sequencing was used to reveal the characteristic taxa and major shifts in the composition of the bacterial communities. Dynamic temporal changes were observed in the structure of bacterial communities. Bacterial richness and diversity increased during the biofilm formation, and 9–12 weeks were needed for the maturation. Increasing EPS production was also observed from the 9–12 weeks. The biofilm was different from the water that filled the cave pool, in terms of the taxonomic composition and metabolic potential of microorganisms. In these karstic environments, the formation of mature biofilm appears to take place relatively quickly, in a few months.https://doi.org/10.1038/s41598-020-78759-4 |
| spellingShingle | Dóra Anda Attila Szabó Petra Kovács-Bodor Judit Makk Tamás Felföldi Éva Ács Judit Mádl-Szőnyi Andrea K. Borsodi In situ modelling of biofilm formation in a hydrothermal spring cave Scientific Reports |
| title | In situ modelling of biofilm formation in a hydrothermal spring cave |
| title_full | In situ modelling of biofilm formation in a hydrothermal spring cave |
| title_fullStr | In situ modelling of biofilm formation in a hydrothermal spring cave |
| title_full_unstemmed | In situ modelling of biofilm formation in a hydrothermal spring cave |
| title_short | In situ modelling of biofilm formation in a hydrothermal spring cave |
| title_sort | in situ modelling of biofilm formation in a hydrothermal spring cave |
| url | https://doi.org/10.1038/s41598-020-78759-4 |
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