The Halophyte Dehydrin Sequence Landscape
Dehydrins (DHNs) belong to the LEA (late embryogenesis abundant) family group II, that comprise four conserved motifs (the Y-, S-, F-, and K-segments) and are known to play a multifunctional role in plant stress tolerance. Based on the presence and order of these segments, dehydrins are divided into...
| Κύριοι συγγραφείς: | , , |
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| Μορφή: | Άρθρο |
| Γλώσσα: | English |
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MDPI AG
2022-02-01
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| Σειρά: | Biomolecules |
| Θέματα: | |
| Διαθέσιμο Online: | https://www.mdpi.com/2218-273X/12/2/330 |
| _version_ | 1827656474819559424 |
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| author | Siwar Ghanmi Steffen P. Graether Moez Hanin |
| author_facet | Siwar Ghanmi Steffen P. Graether Moez Hanin |
| author_sort | Siwar Ghanmi |
| collection | DOAJ |
| description | Dehydrins (DHNs) belong to the LEA (late embryogenesis abundant) family group II, that comprise four conserved motifs (the Y-, S-, F-, and K-segments) and are known to play a multifunctional role in plant stress tolerance. Based on the presence and order of these segments, dehydrins are divided into six subclasses: YnSKn, FnSKn, YnKn, SKn, Kn, and KnS. DHNs are rarely studied in halophytes, and their contribution to the mechanisms developed by these plants to survive in extreme conditions remains unknown. In this work, we carried out multiple genomic analyses of the conservation of halophytic DHN sequences to discover new segments, and examine their architectures, while comparing them with their orthologs in glycophytic plants. We performed an in silico analysis on 86 DHN sequences from 10 halophytic genomes. The phylogenetic tree showed that there are different distributions of the architectures among the different species, and that FSKn is the only architecture present in every plant studied. It was found that K-, F-, Y-, and S-segments are highly conserved in halophytes and glycophytes with a few modifications, mainly involving charged amino acids. Finally, expression data collected for three halophytic species (<i>Puccinillia tenuiflora</i>, <i>Eutrema salsugenium</i>, and <i>Hordeum marinum</i>) revealed that many DHNs are upregulated by salt stress, and the intensity of this upregulation depends on the DHN architecture. |
| first_indexed | 2024-03-09T22:28:57Z |
| format | Article |
| id | doaj.art-6a90f071eb6f4ccd8bde2fca50225ed3 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T22:28:57Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-6a90f071eb6f4ccd8bde2fca50225ed32023-11-23T19:00:12ZengMDPI AGBiomolecules2218-273X2022-02-0112233010.3390/biom12020330The Halophyte Dehydrin Sequence LandscapeSiwar Ghanmi0Steffen P. Graether1Moez Hanin2Plant Physiology & Functional Genomics Research Unit, Institute of Biotechnology, University of Sfax, Sfax 3038, TunisiaDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, CanadaPlant Physiology & Functional Genomics Research Unit, Institute of Biotechnology, University of Sfax, Sfax 3038, TunisiaDehydrins (DHNs) belong to the LEA (late embryogenesis abundant) family group II, that comprise four conserved motifs (the Y-, S-, F-, and K-segments) and are known to play a multifunctional role in plant stress tolerance. Based on the presence and order of these segments, dehydrins are divided into six subclasses: YnSKn, FnSKn, YnKn, SKn, Kn, and KnS. DHNs are rarely studied in halophytes, and their contribution to the mechanisms developed by these plants to survive in extreme conditions remains unknown. In this work, we carried out multiple genomic analyses of the conservation of halophytic DHN sequences to discover new segments, and examine their architectures, while comparing them with their orthologs in glycophytic plants. We performed an in silico analysis on 86 DHN sequences from 10 halophytic genomes. The phylogenetic tree showed that there are different distributions of the architectures among the different species, and that FSKn is the only architecture present in every plant studied. It was found that K-, F-, Y-, and S-segments are highly conserved in halophytes and glycophytes with a few modifications, mainly involving charged amino acids. Finally, expression data collected for three halophytic species (<i>Puccinillia tenuiflora</i>, <i>Eutrema salsugenium</i>, and <i>Hordeum marinum</i>) revealed that many DHNs are upregulated by salt stress, and the intensity of this upregulation depends on the DHN architecture.https://www.mdpi.com/2218-273X/12/2/330halophytic plantsdehydrinin silico analysisabiotic stresssalt stress |
| spellingShingle | Siwar Ghanmi Steffen P. Graether Moez Hanin The Halophyte Dehydrin Sequence Landscape Biomolecules halophytic plants dehydrin in silico analysis abiotic stress salt stress |
| title | The Halophyte Dehydrin Sequence Landscape |
| title_full | The Halophyte Dehydrin Sequence Landscape |
| title_fullStr | The Halophyte Dehydrin Sequence Landscape |
| title_full_unstemmed | The Halophyte Dehydrin Sequence Landscape |
| title_short | The Halophyte Dehydrin Sequence Landscape |
| title_sort | halophyte dehydrin sequence landscape |
| topic | halophytic plants dehydrin in silico analysis abiotic stress salt stress |
| url | https://www.mdpi.com/2218-273X/12/2/330 |
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