Proteomics and Mass Spectrometry for Cancer Biomarker Discovery
Proteomics is a rapidly advancing field not only in the field of biology but also in translational cancer research. In recent years, mass spectrometry and associated technologies have been explored to identify proteins or a set of proteins specific to a given disease, for the purpose of disease dete...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2007-01-01
|
| Series: | Biomarker Insights |
| Subjects: | |
| Online Access: | http://la-press.com/article.php?article_id=385 |
| _version_ | 1819100108190384128 |
|---|---|
| author | Ming Lu Kym F. Faull Julian P. Whitelegge Jianbo He Dejun Shen Romaine E. Saxton Helena R. Chang |
| author_facet | Ming Lu Kym F. Faull Julian P. Whitelegge Jianbo He Dejun Shen Romaine E. Saxton Helena R. Chang |
| author_sort | Ming Lu |
| collection | DOAJ |
| description | Proteomics is a rapidly advancing field not only in the field of biology but also in translational cancer research. In recent years, mass spectrometry and associated technologies have been explored to identify proteins or a set of proteins specific to a given disease, for the purpose of disease detection and diagnosis. Such biomarkers are being investigated in samples including cells, tissues, serum/plasma, and other types of body fluids. When sufficiently refined, proteomic technologies may pave the way for early detection of cancer or individualized therapy for cancer. Mass spectrometry approaches coupled with bioinformatic tools are being developed for biomarker discovery and validation. Understanding basic concepts and application of such technology by investigators in the field may accelerate the clinical application of protein biomarkers in disease management.Abbreviations: 2DE: two-dimensional gel electrophoresis; ABPP: activity-based protein profiling; CEA: carcinoembryonic antigen; CI: confidence interval; ESI: electrospray ionization; FP: fluorophosphonate; HPLC: high performance liquid chromatography; ICAT: isotope coded affi nitytags; IEF: isoelectric focusing; iTRAQ: isobaric tags for relative and absolute quantification; LCMS: combined liquid chromatography-mass spectrometry; LCMSMS: liquid chromatography tandem mass spectrometry; LOD: limit of detection; m/z: mass to charge ratio; MALDI: matrix-assisted laser desorption ionization; MS: mass spectrometry; MUDPIT: multidimensional protein identification technology; NAF: nipple aspirate fluid; PMF: peptide mass fingerprinting; PSA: prostate specifi c antigen; PTMs: post-translational modifications; RPMA: reverse phase protein microarray; SELDI: surface enhanced laser desorption ionization; TOF: time-of-flight. |
| first_indexed | 2024-12-22T00:57:32Z |
| format | Article |
| id | doaj.art-6a9160ff5ad34c79be24167e5a1ba36c |
| institution | Directory Open Access Journal |
| issn | 1177-2719 |
| language | English |
| last_indexed | 2024-12-22T00:57:32Z |
| publishDate | 2007-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Biomarker Insights |
| spelling | doaj.art-6a9160ff5ad34c79be24167e5a1ba36c2022-12-21T18:44:16ZengSAGE PublishingBiomarker Insights1177-27192007-01-012347360Proteomics and Mass Spectrometry for Cancer Biomarker DiscoveryMing LuKym F. FaullJulian P. WhiteleggeJianbo HeDejun ShenRomaine E. SaxtonHelena R. ChangProteomics is a rapidly advancing field not only in the field of biology but also in translational cancer research. In recent years, mass spectrometry and associated technologies have been explored to identify proteins or a set of proteins specific to a given disease, for the purpose of disease detection and diagnosis. Such biomarkers are being investigated in samples including cells, tissues, serum/plasma, and other types of body fluids. When sufficiently refined, proteomic technologies may pave the way for early detection of cancer or individualized therapy for cancer. Mass spectrometry approaches coupled with bioinformatic tools are being developed for biomarker discovery and validation. Understanding basic concepts and application of such technology by investigators in the field may accelerate the clinical application of protein biomarkers in disease management.Abbreviations: 2DE: two-dimensional gel electrophoresis; ABPP: activity-based protein profiling; CEA: carcinoembryonic antigen; CI: confidence interval; ESI: electrospray ionization; FP: fluorophosphonate; HPLC: high performance liquid chromatography; ICAT: isotope coded affi nitytags; IEF: isoelectric focusing; iTRAQ: isobaric tags for relative and absolute quantification; LCMS: combined liquid chromatography-mass spectrometry; LCMSMS: liquid chromatography tandem mass spectrometry; LOD: limit of detection; m/z: mass to charge ratio; MALDI: matrix-assisted laser desorption ionization; MS: mass spectrometry; MUDPIT: multidimensional protein identification technology; NAF: nipple aspirate fluid; PMF: peptide mass fingerprinting; PSA: prostate specifi c antigen; PTMs: post-translational modifications; RPMA: reverse phase protein microarray; SELDI: surface enhanced laser desorption ionization; TOF: time-of-flight.http://la-press.com/article.php?article_id=385ProteomicsMass spectrometryCancerBiomarkers. |
| spellingShingle | Ming Lu Kym F. Faull Julian P. Whitelegge Jianbo He Dejun Shen Romaine E. Saxton Helena R. Chang Proteomics and Mass Spectrometry for Cancer Biomarker Discovery Biomarker Insights Proteomics Mass spectrometry Cancer Biomarkers. |
| title | Proteomics and Mass Spectrometry for Cancer Biomarker Discovery |
| title_full | Proteomics and Mass Spectrometry for Cancer Biomarker Discovery |
| title_fullStr | Proteomics and Mass Spectrometry for Cancer Biomarker Discovery |
| title_full_unstemmed | Proteomics and Mass Spectrometry for Cancer Biomarker Discovery |
| title_short | Proteomics and Mass Spectrometry for Cancer Biomarker Discovery |
| title_sort | proteomics and mass spectrometry for cancer biomarker discovery |
| topic | Proteomics Mass spectrometry Cancer Biomarkers. |
| url | http://la-press.com/article.php?article_id=385 |
| work_keys_str_mv | AT minglu proteomicsandmassspectrometryforcancerbiomarkerdiscovery AT kymffaull proteomicsandmassspectrometryforcancerbiomarkerdiscovery AT julianpwhitelegge proteomicsandmassspectrometryforcancerbiomarkerdiscovery AT jianbohe proteomicsandmassspectrometryforcancerbiomarkerdiscovery AT dejunshen proteomicsandmassspectrometryforcancerbiomarkerdiscovery AT romaineesaxton proteomicsandmassspectrometryforcancerbiomarkerdiscovery AT helenarchang proteomicsandmassspectrometryforcancerbiomarkerdiscovery |