Genetic diversity in some local chicken breeds using microsatellite markers

Genetic relationships among Veneto native breeds of chickens were studied on the basis of microsatellites polymorphisms. A total of 100 DNA samples from 2 local chicken breeds (45 Robusta Lionata and 43 Robusta Maculata) and a commercial broiler line (12 Golden Comet) were analyzed using 19 microsat...

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Main Authors: M. Cassandro, C. Dalvit, M. Gervaso, E. Zanetti
Format: Article
Language:English
Published: Taylor & Francis Group 2010-04-01
Series:Italian Journal of Animal Science
Subjects:
Online Access:http://www.aspajournal.it/index.php/ijas/article/view/1460
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author M. Cassandro
C. Dalvit
M. Gervaso
E. Zanetti
author_facet M. Cassandro
C. Dalvit
M. Gervaso
E. Zanetti
author_sort M. Cassandro
collection DOAJ
description Genetic relationships among Veneto native breeds of chickens were studied on the basis of microsatellites polymorphisms. A total of 100 DNA samples from 2 local chicken breeds (45 Robusta Lionata and 43 Robusta Maculata) and a commercial broiler line (12 Golden Comet) were analyzed using 19 microsatellite markers. The average number of alleles per locus was 4.05 and the expected heterozigosity resulted lower for the local breeds than the broiler line. The Robusta Lionata breed and the broiler line showed a significant deficit and excess of heterozygotes, respectively, deviating from Hardy-Weinberg equilibrium. Nei’s standard genetic distances corrected for bias due to sampling of individuals (Da), based on allele frequencies, were calculated among breeds. The local breeds resulted very similar confirming the same genetic origin. The results suggested that microsatellite markers are a useful tool for studying the genetic diversity among local chicken breeds.
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spelling doaj.art-6a9386779a1c40808941da9e9fc11d532022-12-22T02:01:28ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2010-04-0161s22522710.4081/ijas.2007.1s.225Genetic diversity in some local chicken breeds using microsatellite markersM. CassandroC. DalvitM. GervasoE. ZanettiGenetic relationships among Veneto native breeds of chickens were studied on the basis of microsatellites polymorphisms. A total of 100 DNA samples from 2 local chicken breeds (45 Robusta Lionata and 43 Robusta Maculata) and a commercial broiler line (12 Golden Comet) were analyzed using 19 microsatellite markers. The average number of alleles per locus was 4.05 and the expected heterozigosity resulted lower for the local breeds than the broiler line. The Robusta Lionata breed and the broiler line showed a significant deficit and excess of heterozygotes, respectively, deviating from Hardy-Weinberg equilibrium. Nei’s standard genetic distances corrected for bias due to sampling of individuals (Da), based on allele frequencies, were calculated among breeds. The local breeds resulted very similar confirming the same genetic origin. The results suggested that microsatellite markers are a useful tool for studying the genetic diversity among local chicken breeds.http://www.aspajournal.it/index.php/ijas/article/view/1460Chicken, Genetic distances, Microsatellites, Conservation
spellingShingle M. Cassandro
C. Dalvit
M. Gervaso
E. Zanetti
Genetic diversity in some local chicken breeds using microsatellite markers
Italian Journal of Animal Science
Chicken, Genetic distances, Microsatellites, Conservation
title Genetic diversity in some local chicken breeds using microsatellite markers
title_full Genetic diversity in some local chicken breeds using microsatellite markers
title_fullStr Genetic diversity in some local chicken breeds using microsatellite markers
title_full_unstemmed Genetic diversity in some local chicken breeds using microsatellite markers
title_short Genetic diversity in some local chicken breeds using microsatellite markers
title_sort genetic diversity in some local chicken breeds using microsatellite markers
topic Chicken, Genetic distances, Microsatellites, Conservation
url http://www.aspajournal.it/index.php/ijas/article/view/1460
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AT ezanetti geneticdiversityinsomelocalchickenbreedsusingmicrosatellitemarkers