Genetic diversity in some local chicken breeds using microsatellite markers
Genetic relationships among Veneto native breeds of chickens were studied on the basis of microsatellites polymorphisms. A total of 100 DNA samples from 2 local chicken breeds (45 Robusta Lionata and 43 Robusta Maculata) and a commercial broiler line (12 Golden Comet) were analyzed using 19 microsat...
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Format: | Article |
Language: | English |
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Taylor & Francis Group
2010-04-01
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Series: | Italian Journal of Animal Science |
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Online Access: | http://www.aspajournal.it/index.php/ijas/article/view/1460 |
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author | M. Cassandro C. Dalvit M. Gervaso E. Zanetti |
author_facet | M. Cassandro C. Dalvit M. Gervaso E. Zanetti |
author_sort | M. Cassandro |
collection | DOAJ |
description | Genetic relationships among Veneto native breeds of chickens were studied on the basis of microsatellites polymorphisms. A total of 100 DNA samples from 2 local chicken breeds (45 Robusta Lionata and 43 Robusta Maculata) and a commercial broiler line (12 Golden Comet) were analyzed using 19 microsatellite markers. The average number of alleles per locus was 4.05 and the expected heterozigosity resulted lower for the local breeds than the broiler line. The Robusta Lionata breed and the broiler line showed a significant deficit and excess of heterozygotes, respectively, deviating from Hardy-Weinberg equilibrium. Nei’s standard genetic distances corrected for bias due to sampling of individuals (Da), based on allele frequencies, were calculated among breeds. The local breeds resulted very similar confirming the same genetic origin. The results suggested that microsatellite markers are a useful tool for studying the genetic diversity among local chicken breeds. |
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id | doaj.art-6a9386779a1c40808941da9e9fc11d53 |
institution | Directory Open Access Journal |
issn | 1594-4077 1828-051X |
language | English |
last_indexed | 2024-12-10T04:57:06Z |
publishDate | 2010-04-01 |
publisher | Taylor & Francis Group |
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series | Italian Journal of Animal Science |
spelling | doaj.art-6a9386779a1c40808941da9e9fc11d532022-12-22T02:01:28ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2010-04-0161s22522710.4081/ijas.2007.1s.225Genetic diversity in some local chicken breeds using microsatellite markersM. CassandroC. DalvitM. GervasoE. ZanettiGenetic relationships among Veneto native breeds of chickens were studied on the basis of microsatellites polymorphisms. A total of 100 DNA samples from 2 local chicken breeds (45 Robusta Lionata and 43 Robusta Maculata) and a commercial broiler line (12 Golden Comet) were analyzed using 19 microsatellite markers. The average number of alleles per locus was 4.05 and the expected heterozigosity resulted lower for the local breeds than the broiler line. The Robusta Lionata breed and the broiler line showed a significant deficit and excess of heterozygotes, respectively, deviating from Hardy-Weinberg equilibrium. Nei’s standard genetic distances corrected for bias due to sampling of individuals (Da), based on allele frequencies, were calculated among breeds. The local breeds resulted very similar confirming the same genetic origin. The results suggested that microsatellite markers are a useful tool for studying the genetic diversity among local chicken breeds.http://www.aspajournal.it/index.php/ijas/article/view/1460Chicken, Genetic distances, Microsatellites, Conservation |
spellingShingle | M. Cassandro C. Dalvit M. Gervaso E. Zanetti Genetic diversity in some local chicken breeds using microsatellite markers Italian Journal of Animal Science Chicken, Genetic distances, Microsatellites, Conservation |
title | Genetic diversity in some local chicken breeds using microsatellite markers |
title_full | Genetic diversity in some local chicken breeds using microsatellite markers |
title_fullStr | Genetic diversity in some local chicken breeds using microsatellite markers |
title_full_unstemmed | Genetic diversity in some local chicken breeds using microsatellite markers |
title_short | Genetic diversity in some local chicken breeds using microsatellite markers |
title_sort | genetic diversity in some local chicken breeds using microsatellite markers |
topic | Chicken, Genetic distances, Microsatellites, Conservation |
url | http://www.aspajournal.it/index.php/ijas/article/view/1460 |
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