Identification and validation of the role of c-Myc in head and neck squamous cell carcinoma
BackgroundMany studies have shown that c-Myc plays a critical role in tumorigenesis. However, the molecular role of c-Myc in head and neck squamous cell carcinoma (HNSC) remains unclear.MethodsSeveral biological databases, including UALCAN, TIMER2.0, TCGAportal, GEPIA, KM plotter, OncoLnc, LinkedOmi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.820587/full |
| _version_ | 1828357195200200704 |
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| author | Sufeng Zhao Li An Xudong Yang Zheng Wei He Zhang Yufeng Wang |
| author_facet | Sufeng Zhao Li An Xudong Yang Zheng Wei He Zhang Yufeng Wang |
| author_sort | Sufeng Zhao |
| collection | DOAJ |
| description | BackgroundMany studies have shown that c-Myc plays a critical role in tumorigenesis. However, the molecular role of c-Myc in head and neck squamous cell carcinoma (HNSC) remains unclear.MethodsSeveral biological databases, including UALCAN, TIMER2.0, TCGAportal, GEPIA, KM plotter, OncoLnc, LinkedOmics, GSCA, and TCIA, were used to analyze the molecular role of c-Myc in HNSC. The expression levels of c-Myc were validated by real-time PCR (RT–PCR) and Western blot in CAL-27 cells.ResultsThe expression of c-Myc mRNA were significantly increased in HPV-negative HNSC tissues. The expression of c-Myc gene level was correlated with TP53 mutation status. HNSC also showed hypomethylated c-Myc compared with normal tissues. c-Myc was identified as an ominous prognostic factor for HNSC patients and correlated with immune infiltrating levels. Moreover, high c-Myc expression was associated with decreased expression of a series of immune checkpoints, resulting in a dampened immune response. c-Myc potentially mediated IL-17 signaling pathway and Th1 and Th2 cell differentiation. Inhibition of c-Myc expression increased apoptosis of CAL-27 cells.ConclusionsThese findings suggest a new mechanism of c-Myc in the prognosis of HNSC, implying the potential of c-Myc as a therapeutic target for HNSC patients. |
| first_indexed | 2024-04-14T03:11:37Z |
| format | Article |
| id | doaj.art-6a9471a652694a0e878811f3810bb5e8 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-14T03:11:37Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-6a9471a652694a0e878811f3810bb5e82022-12-22T02:15:35ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-08-011210.3389/fonc.2022.820587820587Identification and validation of the role of c-Myc in head and neck squamous cell carcinomaSufeng Zhao0Li An1Xudong Yang2Zheng Wei3He Zhang4Yufeng Wang5Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Geriatrics, Zhongda Hospital Southeast University, Nanjing, ChinaDepartment of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Pediatric Dentistry, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaBackgroundMany studies have shown that c-Myc plays a critical role in tumorigenesis. However, the molecular role of c-Myc in head and neck squamous cell carcinoma (HNSC) remains unclear.MethodsSeveral biological databases, including UALCAN, TIMER2.0, TCGAportal, GEPIA, KM plotter, OncoLnc, LinkedOmics, GSCA, and TCIA, were used to analyze the molecular role of c-Myc in HNSC. The expression levels of c-Myc were validated by real-time PCR (RT–PCR) and Western blot in CAL-27 cells.ResultsThe expression of c-Myc mRNA were significantly increased in HPV-negative HNSC tissues. The expression of c-Myc gene level was correlated with TP53 mutation status. HNSC also showed hypomethylated c-Myc compared with normal tissues. c-Myc was identified as an ominous prognostic factor for HNSC patients and correlated with immune infiltrating levels. Moreover, high c-Myc expression was associated with decreased expression of a series of immune checkpoints, resulting in a dampened immune response. c-Myc potentially mediated IL-17 signaling pathway and Th1 and Th2 cell differentiation. Inhibition of c-Myc expression increased apoptosis of CAL-27 cells.ConclusionsThese findings suggest a new mechanism of c-Myc in the prognosis of HNSC, implying the potential of c-Myc as a therapeutic target for HNSC patients.https://www.frontiersin.org/articles/10.3389/fonc.2022.820587/fullhead and neck squamous cell carcinomac-MycsurvivalmRNAtriptonide |
| spellingShingle | Sufeng Zhao Li An Xudong Yang Zheng Wei He Zhang Yufeng Wang Identification and validation of the role of c-Myc in head and neck squamous cell carcinoma Frontiers in Oncology head and neck squamous cell carcinoma c-Myc survival mRNA triptonide |
| title | Identification and validation of the role of c-Myc in head and neck squamous cell carcinoma |
| title_full | Identification and validation of the role of c-Myc in head and neck squamous cell carcinoma |
| title_fullStr | Identification and validation of the role of c-Myc in head and neck squamous cell carcinoma |
| title_full_unstemmed | Identification and validation of the role of c-Myc in head and neck squamous cell carcinoma |
| title_short | Identification and validation of the role of c-Myc in head and neck squamous cell carcinoma |
| title_sort | identification and validation of the role of c myc in head and neck squamous cell carcinoma |
| topic | head and neck squamous cell carcinoma c-Myc survival mRNA triptonide |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.820587/full |
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