Measuring Solution Viscosity and its Effect on Enzyme Activity
<p>In proteins, some processes require conformational changes involving structural domain diffusion. Among these processes are protein folding, unfolding and enzyme catalysis. During catalysis some enzymes undergo large conformational changes as they progress through the catalytic cycle. Accor...
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| Format: | Article |
| Language: | English |
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BMC
2003-01-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/52/m52.htm |
| _version_ | 1811295298495971328 |
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| author | Uribe Salvador Sampedro José G. |
| author_facet | Uribe Salvador Sampedro José G. |
| author_sort | Uribe Salvador |
| collection | DOAJ |
| description | <p>In proteins, some processes require conformational changes involving structural domain diffusion. Among these processes are protein folding, unfolding and enzyme catalysis. During catalysis some enzymes undergo large conformational changes as they progress through the catalytic cycle. According to Kramers theory, solvent viscosity results in friction against proteins in solution, and this should result in decreased motion, inhibiting catalysis in motile enzymes. Solution viscosity was increased by adding increasing concentrations of glycerol, sucrose and trehalose, resulting in a decrease in the reaction rate of the H<sup>+</sup>-ATPase from the plasma membrane of <it>Kluyveromyces lactis.</it> A direct correlation was found between viscosity (&eegr;) and the inhibition of the maximum rate of catalysis (<it>V</it> <sub>max</sub>). The protocol used to measure viscosity by means of a falling ball type viscometer is described, together with the determination of enzyme kinetics and the application of Kramers’ equation to evaluate the effect of viscosity on the rate of ATP hydrolysis by the H<sup>+</sup>-ATPase. |
| first_indexed | 2024-04-13T05:31:12Z |
| format | Article |
| id | doaj.art-6a95ac7eae7e43a8a1a1c32a03b39fb6 |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-04-13T05:31:12Z |
| publishDate | 2003-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-6a95ac7eae7e43a8a1a1c32a03b39fb62022-12-22T03:00:26ZengBMCBiological Procedures Online1480-92222003-01-015110811510.1251/bpo52Measuring Solution Viscosity and its Effect on Enzyme ActivityUribe SalvadorSampedro José G.<p>In proteins, some processes require conformational changes involving structural domain diffusion. Among these processes are protein folding, unfolding and enzyme catalysis. During catalysis some enzymes undergo large conformational changes as they progress through the catalytic cycle. According to Kramers theory, solvent viscosity results in friction against proteins in solution, and this should result in decreased motion, inhibiting catalysis in motile enzymes. Solution viscosity was increased by adding increasing concentrations of glycerol, sucrose and trehalose, resulting in a decrease in the reaction rate of the H<sup>+</sup>-ATPase from the plasma membrane of <it>Kluyveromyces lactis.</it> A direct correlation was found between viscosity (&eegr;) and the inhibition of the maximum rate of catalysis (<it>V</it> <sub>max</sub>). The protocol used to measure viscosity by means of a falling ball type viscometer is described, together with the determination of enzyme kinetics and the application of Kramers’ equation to evaluate the effect of viscosity on the rate of ATP hydrolysis by the H<sup>+</sup>-ATPase.http://www.biologicalprocedures.com/bpo/arts/1/52/m52.htmViscosityTrehalose |
| spellingShingle | Uribe Salvador Sampedro José G. Measuring Solution Viscosity and its Effect on Enzyme Activity Biological Procedures Online Viscosity Trehalose |
| title | Measuring Solution Viscosity and its Effect on Enzyme Activity |
| title_full | Measuring Solution Viscosity and its Effect on Enzyme Activity |
| title_fullStr | Measuring Solution Viscosity and its Effect on Enzyme Activity |
| title_full_unstemmed | Measuring Solution Viscosity and its Effect on Enzyme Activity |
| title_short | Measuring Solution Viscosity and its Effect on Enzyme Activity |
| title_sort | measuring solution viscosity and its effect on enzyme activity |
| topic | Viscosity Trehalose |
| url | http://www.biologicalprocedures.com/bpo/arts/1/52/m52.htm |
| work_keys_str_mv | AT uribesalvador measuringsolutionviscosityanditseffectonenzymeactivity AT sampedrojoseacuteg measuringsolutionviscosityanditseffectonenzymeactivity |