In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19α Gene Expression in Zebrafish Danio rerio
In this study, we tested the compatibility of two extracts from the plant Jerusalem artichokes and button mushrooms with two different Lactobacillus probiotics (Lactobacillus acidophilus; La and Lactobacillus delbrueckii subsp. Bulgaricus; Lb) to develop a synbiotic formulation to improve the growth...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.758758/full |
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| author | Hamideh Zakariaee Mohammad Sudagar Seyede Sedighe Hosseini Seyede Sedighe Hosseini Hamed Paknejad Kartik Baruah |
| author_facet | Hamideh Zakariaee Mohammad Sudagar Seyede Sedighe Hosseini Seyede Sedighe Hosseini Hamed Paknejad Kartik Baruah |
| author_sort | Hamideh Zakariaee |
| collection | DOAJ |
| description | In this study, we tested the compatibility of two extracts from the plant Jerusalem artichokes and button mushrooms with two different Lactobacillus probiotics (Lactobacillus acidophilus; La and Lactobacillus delbrueckii subsp. Bulgaricus; Lb) to develop a synbiotic formulation to improve the growth, survival, and reproductive performances of farmed fishes. Initially, we employed in vitro approach to monitor the growth of the probiotic lactobacilli in the presence of the different doses of the plant-based prebiotics, with the aim of selecting interesting combination(s) for further verification under in vivo conditions using zebrafish as a model. Results from the in vitro screening assay in the broth showed that both the probiotic species showed a preference for 50% mushroom extract as a source of prebiotic. A synbiotic formulation, developed with the selected combination of L. acidophilus, L. bulgaricus, and 50% mushroom extract, showed a positive influence on the growth and reproductive performances of the zebrafish. Our findings also imply that the improvement in the reproductive indices was associated with the upregulation of a cyp19a gene. Overall results suggest that a combination of L. acidophilus, L. bulgaricus, and mushroom extract can be considered as a potential synbiotic for the successful production of aquaculture species. |
| first_indexed | 2024-12-19T00:24:01Z |
| format | Article |
| id | doaj.art-6a9792858e1d4edfba5c41311aa3a1c7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T00:24:01Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6a9792858e1d4edfba5c41311aa3a1c72022-12-21T20:45:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-09-011210.3389/fmicb.2021.758758758758In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19α Gene Expression in Zebrafish Danio rerioHamideh Zakariaee0Mohammad Sudagar1Seyede Sedighe Hosseini2Seyede Sedighe Hosseini3Hamed Paknejad4Kartik Baruah5Department of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agriculture Sciences and Natural Resources, Gorgan, IranDepartment of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agriculture Sciences and Natural Resources, Gorgan, IranLaboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, IranDepartment of Laboratory Sciences, Faculty of Paramedicine, Golestan University of Medical Sciences, Gorgan, IranDepartment of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agriculture Sciences and Natural Resources, Gorgan, IranDepartment of Animal Nutrition and Management, Aquaculture Nutraceuticals Research Group, Faculty of Veterinary Medicine and Animal Sciences, Swedish University of Agricultural Sciences, Uppsala, SwedenIn this study, we tested the compatibility of two extracts from the plant Jerusalem artichokes and button mushrooms with two different Lactobacillus probiotics (Lactobacillus acidophilus; La and Lactobacillus delbrueckii subsp. Bulgaricus; Lb) to develop a synbiotic formulation to improve the growth, survival, and reproductive performances of farmed fishes. Initially, we employed in vitro approach to monitor the growth of the probiotic lactobacilli in the presence of the different doses of the plant-based prebiotics, with the aim of selecting interesting combination(s) for further verification under in vivo conditions using zebrafish as a model. Results from the in vitro screening assay in the broth showed that both the probiotic species showed a preference for 50% mushroom extract as a source of prebiotic. A synbiotic formulation, developed with the selected combination of L. acidophilus, L. bulgaricus, and 50% mushroom extract, showed a positive influence on the growth and reproductive performances of the zebrafish. Our findings also imply that the improvement in the reproductive indices was associated with the upregulation of a cyp19a gene. Overall results suggest that a combination of L. acidophilus, L. bulgaricus, and mushroom extract can be considered as a potential synbiotic for the successful production of aquaculture species.https://www.frontiersin.org/articles/10.3389/fmicb.2021.758758/fullprebioticsprobioticszebrafishmushroomartichokereproduction |
| spellingShingle | Hamideh Zakariaee Mohammad Sudagar Seyede Sedighe Hosseini Seyede Sedighe Hosseini Hamed Paknejad Kartik Baruah In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19α Gene Expression in Zebrafish Danio rerio Frontiers in Microbiology prebiotics probiotics zebrafish mushroom artichoke reproduction |
| title | In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19α Gene Expression in Zebrafish Danio rerio |
| title_full | In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19α Gene Expression in Zebrafish Danio rerio |
| title_fullStr | In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19α Gene Expression in Zebrafish Danio rerio |
| title_full_unstemmed | In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19α Gene Expression in Zebrafish Danio rerio |
| title_short | In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19α Gene Expression in Zebrafish Danio rerio |
| title_sort | in vitro selection of synbiotics and in vivo investigation of growth indices reproduction performance survival and ovarian cyp19α gene expression in zebrafish danio rerio |
| topic | prebiotics probiotics zebrafish mushroom artichoke reproduction |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.758758/full |
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