Inhibitory Activity of the Flower Buds of Lonicera japonica Thunb. against Histamine Production and L-Histidine Decarboxylase in Human Keratinocytes
In previous studies we found that anionic surfactants such as sodium laurate (SL) and/or sodium dodecylsulfate (SDS) exert actions on epidermal keratinocytes rather than mast cells to give rise of histamine production and skin itching through increasing the expression of the 53-kDa active form of l-...
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MDPI AG
2014-06-01
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| Series: | Molecules |
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| author | Yoshihiro Inami Yuko Matsui Tomoko Hoshino Chiaki Murayama Hisayoshi Norimoto |
| author_facet | Yoshihiro Inami Yuko Matsui Tomoko Hoshino Chiaki Murayama Hisayoshi Norimoto |
| author_sort | Yoshihiro Inami |
| collection | DOAJ |
| description | In previous studies we found that anionic surfactants such as sodium laurate (SL) and/or sodium dodecylsulfate (SDS) exert actions on epidermal keratinocytes rather than mast cells to give rise of histamine production and skin itching through increasing the expression of the 53-kDa active form of l-histidine decarboxylase (HDC). In addition, with treatment of SL in a three-dimensional human keratinocyte culture, increases in both the 53-kDa HDC and histamine production are detected and thus this culture assay is applied to screen anti-itching materials from natural resources. In this study, the inhibitory activity of “Kin-gin-ka” (flower buds of Lonicera japonica Thunb., FLJ) against histamine production and expression of the active form of HDC were examined in this culture assay. FLJ is a well-known traditional Chinese medicine, being used to treat fevers, coughs and some infectious diseases. The result showed both FLJ and chlorogenic acid had inhibitory activities against the expression of 53-kDa HDC and histamine production. However, chlorogenic acid showed a weaker effect on histamine production than that of FLJ, suggesting that other chemical constituents besides chlorogenic acid could contribute to the inhibitory activities. Thus, a further chemical study of FLJ is now under investigation. |
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| last_indexed | 2024-12-12T11:04:25Z |
| publishDate | 2014-06-01 |
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| spelling | doaj.art-6a9884b5bad947d6ad1463302edd132c2022-12-22T00:26:26ZengMDPI AGMolecules1420-30492014-06-011968212821910.3390/molecules19068212molecules19068212Inhibitory Activity of the Flower Buds of Lonicera japonica Thunb. against Histamine Production and L-Histidine Decarboxylase in Human KeratinocytesYoshihiro Inami0Yuko Matsui1Tomoko Hoshino2Chiaki Murayama3Hisayoshi Norimoto4Fundamental Research Laboratories, Hoyu Co., Ltd., Roboku 1-12, Nagakute City Aichi 480-1136, JapanFundamental Research Laboratories, Hoyu Co., Ltd., Roboku 1-12, Nagakute City Aichi 480-1136, JapanFundamental Research Laboratories, Hoyu Co., Ltd., Roboku 1-12, Nagakute City Aichi 480-1136, JapanKampo Research Laboratories, Kracie Pharma., Ltd., Kanebo machi 3-1, Takaoka City Toyama 933-0856, JapanKampo Research Laboratories, Kracie Pharma., Ltd., Kanebo machi 3-1, Takaoka City Toyama 933-0856, JapanIn previous studies we found that anionic surfactants such as sodium laurate (SL) and/or sodium dodecylsulfate (SDS) exert actions on epidermal keratinocytes rather than mast cells to give rise of histamine production and skin itching through increasing the expression of the 53-kDa active form of l-histidine decarboxylase (HDC). In addition, with treatment of SL in a three-dimensional human keratinocyte culture, increases in both the 53-kDa HDC and histamine production are detected and thus this culture assay is applied to screen anti-itching materials from natural resources. In this study, the inhibitory activity of “Kin-gin-ka” (flower buds of Lonicera japonica Thunb., FLJ) against histamine production and expression of the active form of HDC were examined in this culture assay. FLJ is a well-known traditional Chinese medicine, being used to treat fevers, coughs and some infectious diseases. The result showed both FLJ and chlorogenic acid had inhibitory activities against the expression of 53-kDa HDC and histamine production. However, chlorogenic acid showed a weaker effect on histamine production than that of FLJ, suggesting that other chemical constituents besides chlorogenic acid could contribute to the inhibitory activities. Thus, a further chemical study of FLJ is now under investigation.http://www.mdpi.com/1420-3049/19/6/8212flower buds of Lonicera japonica Thunb.chlorogenic acidhistamineL-histidine decarboxylasehuman keratinocytes |
| spellingShingle | Yoshihiro Inami Yuko Matsui Tomoko Hoshino Chiaki Murayama Hisayoshi Norimoto Inhibitory Activity of the Flower Buds of Lonicera japonica Thunb. against Histamine Production and L-Histidine Decarboxylase in Human Keratinocytes Molecules flower buds of Lonicera japonica Thunb. chlorogenic acid histamine L-histidine decarboxylase human keratinocytes |
| title | Inhibitory Activity of the Flower Buds of Lonicera japonica Thunb. against Histamine Production and L-Histidine Decarboxylase in Human Keratinocytes |
| title_full | Inhibitory Activity of the Flower Buds of Lonicera japonica Thunb. against Histamine Production and L-Histidine Decarboxylase in Human Keratinocytes |
| title_fullStr | Inhibitory Activity of the Flower Buds of Lonicera japonica Thunb. against Histamine Production and L-Histidine Decarboxylase in Human Keratinocytes |
| title_full_unstemmed | Inhibitory Activity of the Flower Buds of Lonicera japonica Thunb. against Histamine Production and L-Histidine Decarboxylase in Human Keratinocytes |
| title_short | Inhibitory Activity of the Flower Buds of Lonicera japonica Thunb. against Histamine Production and L-Histidine Decarboxylase in Human Keratinocytes |
| title_sort | inhibitory activity of the flower buds of lonicera japonica thunb against histamine production and l histidine decarboxylase in human keratinocytes |
| topic | flower buds of Lonicera japonica Thunb. chlorogenic acid histamine L-histidine decarboxylase human keratinocytes |
| url | http://www.mdpi.com/1420-3049/19/6/8212 |
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