Responses of Anabaena sp. PCC7120 to lindane: Physiological effects and differential expression of potential lin genes
Abstract Lindane (γ‐HCH) is an organochlorine pesticide that causes huge environmental concerns worldwide due to its recalcitrance and toxicity. The use of the cyanobacterium Anabaena sp. PCC 7120 in aquatic lindane bioremediation has been suggested but information relative to this process is scarce...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | MicrobiologyOpen |
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| Online Access: | https://doi.org/10.1002/mbo3.1355 |
| _version_ | 1797793306811301888 |
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| author | Jorge Guío Maria F. Fillat Maria L. Peleato Emma Sevilla |
| author_facet | Jorge Guío Maria F. Fillat Maria L. Peleato Emma Sevilla |
| author_sort | Jorge Guío |
| collection | DOAJ |
| description | Abstract Lindane (γ‐HCH) is an organochlorine pesticide that causes huge environmental concerns worldwide due to its recalcitrance and toxicity. The use of the cyanobacterium Anabaena sp. PCC 7120 in aquatic lindane bioremediation has been suggested but information relative to this process is scarce. In the present work, data relative to the growth, pigment composition, photosynthetic/respiration rate, and oxidative stress response of Anabaena sp. PCC 7120 in the presence of lindane at its solubility limit in water are shown. In addition, lindane degradation experiments revealed almost a total disappearance of lindane in the supernatants of Anabaena sp. PCC 7120 culture after 6 days of incubation. The diminishing in lindane concentration was in concordance with an increase in the levels of trichlorobenzene inside the cells. Furthermore, to identify potential orthologs of the linA, linB, linC, linD, linE, and linR genes from Sphingomonas paucimobilis B90A in Anabaena sp. PCC 7120, a whole genome screening was performed allowing the identification of five putative lin orthologs (all1353 and all0193 putative orthologs of linB, all3836 putative orthologs of linC, and all0352 and alr0353 putative orthologs of linE and linR, respectively) which could be involved in the lindane degradation pathway. Differential expression analysis of these genes in the presence of lindane revealed strong upregulation of one of the potential lin genes of Anabaena sp. PCC 7120. |
| first_indexed | 2024-03-13T02:46:24Z |
| format | Article |
| id | doaj.art-6a9d2bc2ecf84dea878c59de6f475817 |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-03-13T02:46:24Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-6a9d2bc2ecf84dea878c59de6f4758172023-06-28T18:00:37ZengWileyMicrobiologyOpen2045-88272023-06-01123n/an/a10.1002/mbo3.1355Responses of Anabaena sp. PCC7120 to lindane: Physiological effects and differential expression of potential lin genesJorge Guío0Maria F. Fillat1Maria L. Peleato2Emma Sevilla3Departamento de Bioquímica y Biología Molecular y Celular and Institute for Biocomputation and Physics of Complex Systems Universidad de Zaragoza Zaragoza SpainDepartamento de Bioquímica y Biología Molecular y Celular and Institute for Biocomputation and Physics of Complex Systems Universidad de Zaragoza Zaragoza SpainDepartamento de Bioquímica y Biología Molecular y Celular and Institute for Biocomputation and Physics of Complex Systems Universidad de Zaragoza Zaragoza SpainDepartamento de Bioquímica y Biología Molecular y Celular and Institute for Biocomputation and Physics of Complex Systems Universidad de Zaragoza Zaragoza SpainAbstract Lindane (γ‐HCH) is an organochlorine pesticide that causes huge environmental concerns worldwide due to its recalcitrance and toxicity. The use of the cyanobacterium Anabaena sp. PCC 7120 in aquatic lindane bioremediation has been suggested but information relative to this process is scarce. In the present work, data relative to the growth, pigment composition, photosynthetic/respiration rate, and oxidative stress response of Anabaena sp. PCC 7120 in the presence of lindane at its solubility limit in water are shown. In addition, lindane degradation experiments revealed almost a total disappearance of lindane in the supernatants of Anabaena sp. PCC 7120 culture after 6 days of incubation. The diminishing in lindane concentration was in concordance with an increase in the levels of trichlorobenzene inside the cells. Furthermore, to identify potential orthologs of the linA, linB, linC, linD, linE, and linR genes from Sphingomonas paucimobilis B90A in Anabaena sp. PCC 7120, a whole genome screening was performed allowing the identification of five putative lin orthologs (all1353 and all0193 putative orthologs of linB, all3836 putative orthologs of linC, and all0352 and alr0353 putative orthologs of linE and linR, respectively) which could be involved in the lindane degradation pathway. Differential expression analysis of these genes in the presence of lindane revealed strong upregulation of one of the potential lin genes of Anabaena sp. PCC 7120.https://doi.org/10.1002/mbo3.1355aquatic bacteriabiodegradationcyanobacteriaenvironmental microbiology |
| spellingShingle | Jorge Guío Maria F. Fillat Maria L. Peleato Emma Sevilla Responses of Anabaena sp. PCC7120 to lindane: Physiological effects and differential expression of potential lin genes MicrobiologyOpen aquatic bacteria biodegradation cyanobacteria environmental microbiology |
| title | Responses of Anabaena sp. PCC7120 to lindane: Physiological effects and differential expression of potential lin genes |
| title_full | Responses of Anabaena sp. PCC7120 to lindane: Physiological effects and differential expression of potential lin genes |
| title_fullStr | Responses of Anabaena sp. PCC7120 to lindane: Physiological effects and differential expression of potential lin genes |
| title_full_unstemmed | Responses of Anabaena sp. PCC7120 to lindane: Physiological effects and differential expression of potential lin genes |
| title_short | Responses of Anabaena sp. PCC7120 to lindane: Physiological effects and differential expression of potential lin genes |
| title_sort | responses of anabaena sp pcc7120 to lindane physiological effects and differential expression of potential lin genes |
| topic | aquatic bacteria biodegradation cyanobacteria environmental microbiology |
| url | https://doi.org/10.1002/mbo3.1355 |
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