Halloween genes AhCYP307A2 and AhCYP314A1 modulate last instar larva–pupa–adult transition, ovarian development and oogenesis in Agasicles hygrophila (Coleoptera: Chrysomelidae)
In insects, ecdysteroids are synthesized by genes of the Halloween family and play important roles in several key developmental events, including molting and metamorphosis. However, the roles of these genes in Agasicles hygrophila are still largely unknown. In this study, the expression patterns of...
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| Format: | Article |
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Elsevier
2023-03-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311922000995 |
| _version_ | 1797869842706989056 |
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| author | Ji-su JIN Yi-ran LIU Zhong-shi ZHOU Fang-hao WAN Jian-ying GUO |
| author_facet | Ji-su JIN Yi-ran LIU Zhong-shi ZHOU Fang-hao WAN Jian-ying GUO |
| author_sort | Ji-su JIN |
| collection | DOAJ |
| description | In insects, ecdysteroids are synthesized by genes of the Halloween family and play important roles in several key developmental events, including molting and metamorphosis. However, the roles of these genes in Agasicles hygrophila are still largely unknown. In this study, the expression patterns of the two Halloween genes AhCYP307A2 and AhCYP314A1 were determined by quantitative PCR (qPCR) at different developmental stages. Moreover, the functions of these two genes were explored using RNA interference (RNAi), and ovarian development was observed by dissecting the ovaries of A. hygrophila females. The qPCR results showed that AhCYP307A2 and AhCYP314A1 were highly expressed in last instar larvae and in adult females. In addition, AhCYP307A2 was also highly expressed in eggs and pupae but was markedly lower than in third-instar larvae and females. The RNAi results showed that the injection of dsAhCYP307A2 or dsAhCYP314A1 markedly inhibited their expression and the transcription levels of three related AhVgs. Knockdown of AhCYP307A2 or AhCYP314A1 significantly inhibited larval molting, impaired last instar larva–pupa–adult transition, delayed ovarian development, and stopped egg production (i.e., no eggs were laid). These results indicate that AhCYP307A2 and AhCYP314A1 play important regulatory roles in last instar larva–pupa–adult transition and reproduction in A. hygrophila. |
| first_indexed | 2024-04-10T00:17:55Z |
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| id | doaj.art-6a9e8b24f25c4e24a4d367bb28b2a1bf |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-04-10T00:17:55Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
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| series | Journal of Integrative Agriculture |
| spelling | doaj.art-6a9e8b24f25c4e24a4d367bb28b2a1bf2023-03-16T05:03:41ZengElsevierJournal of Integrative Agriculture2095-31192023-03-01223812824Halloween genes AhCYP307A2 and AhCYP314A1 modulate last instar larva–pupa–adult transition, ovarian development and oogenesis in Agasicles hygrophila (Coleoptera: Chrysomelidae)Ji-su JIN0Yi-ran LIU1Zhong-shi ZHOU2Fang-hao WAN3Jian-ying GUO4State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China; Correspondence GUO Jian-ying, Tel: +86-10-82109572In insects, ecdysteroids are synthesized by genes of the Halloween family and play important roles in several key developmental events, including molting and metamorphosis. However, the roles of these genes in Agasicles hygrophila are still largely unknown. In this study, the expression patterns of the two Halloween genes AhCYP307A2 and AhCYP314A1 were determined by quantitative PCR (qPCR) at different developmental stages. Moreover, the functions of these two genes were explored using RNA interference (RNAi), and ovarian development was observed by dissecting the ovaries of A. hygrophila females. The qPCR results showed that AhCYP307A2 and AhCYP314A1 were highly expressed in last instar larvae and in adult females. In addition, AhCYP307A2 was also highly expressed in eggs and pupae but was markedly lower than in third-instar larvae and females. The RNAi results showed that the injection of dsAhCYP307A2 or dsAhCYP314A1 markedly inhibited their expression and the transcription levels of three related AhVgs. Knockdown of AhCYP307A2 or AhCYP314A1 significantly inhibited larval molting, impaired last instar larva–pupa–adult transition, delayed ovarian development, and stopped egg production (i.e., no eggs were laid). These results indicate that AhCYP307A2 and AhCYP314A1 play important regulatory roles in last instar larva–pupa–adult transition and reproduction in A. hygrophila.http://www.sciencedirect.com/science/article/pii/S2095311922000995Agasicles hygrophilaovaryoogenesisAhCYP307A2AhCYP314A1RNA interference |
| spellingShingle | Ji-su JIN Yi-ran LIU Zhong-shi ZHOU Fang-hao WAN Jian-ying GUO Halloween genes AhCYP307A2 and AhCYP314A1 modulate last instar larva–pupa–adult transition, ovarian development and oogenesis in Agasicles hygrophila (Coleoptera: Chrysomelidae) Journal of Integrative Agriculture Agasicles hygrophila ovary oogenesis AhCYP307A2 AhCYP314A1 RNA interference |
| title | Halloween genes AhCYP307A2 and AhCYP314A1 modulate last instar larva–pupa–adult transition, ovarian development and oogenesis in Agasicles hygrophila (Coleoptera: Chrysomelidae) |
| title_full | Halloween genes AhCYP307A2 and AhCYP314A1 modulate last instar larva–pupa–adult transition, ovarian development and oogenesis in Agasicles hygrophila (Coleoptera: Chrysomelidae) |
| title_fullStr | Halloween genes AhCYP307A2 and AhCYP314A1 modulate last instar larva–pupa–adult transition, ovarian development and oogenesis in Agasicles hygrophila (Coleoptera: Chrysomelidae) |
| title_full_unstemmed | Halloween genes AhCYP307A2 and AhCYP314A1 modulate last instar larva–pupa–adult transition, ovarian development and oogenesis in Agasicles hygrophila (Coleoptera: Chrysomelidae) |
| title_short | Halloween genes AhCYP307A2 and AhCYP314A1 modulate last instar larva–pupa–adult transition, ovarian development and oogenesis in Agasicles hygrophila (Coleoptera: Chrysomelidae) |
| title_sort | halloween genes ahcyp307a2 and ahcyp314a1 modulate last instar larva pupa adult transition ovarian development and oogenesis in agasicles hygrophila coleoptera chrysomelidae |
| topic | Agasicles hygrophila ovary oogenesis AhCYP307A2 AhCYP314A1 RNA interference |
| url | http://www.sciencedirect.com/science/article/pii/S2095311922000995 |
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