Effects of cadmium on nucleolus in root tips of <em>Hordeum vulgare</em>
Background Hordeum vulgare is an economically important crop that is consumed in large quantities in the world. In a previous investigation, we found that cadmium (Cd) could induce some nucleolar materials containing the argyrophilic proteins scattered in the nuclei and located in the cytoplasm in...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedad Botánica de México, A. C.
2017-03-01
|
| Series: | Botanical Sciences |
| Subjects: | |
| Online Access: | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/694 |
| _version_ | 1828128943738191872 |
|---|---|
| author | Qiuyue Shi Junran Wang Ze Jiang Hangfeng Wu Jiayue Wang Wusheng Jiang Donghua Liu Jinhua Zou |
| author_facet | Qiuyue Shi Junran Wang Ze Jiang Hangfeng Wu Jiayue Wang Wusheng Jiang Donghua Liu Jinhua Zou |
| author_sort | Qiuyue Shi |
| collection | DOAJ |
| description |
Background Hordeum vulgare is an economically important crop that is consumed in large quantities in the world. In a previous investigation, we found that cadmium (Cd) could induce some nucleolar materials containing the argyrophilic proteins scattered in the nuclei and located in the cytoplasm in the root tip cells of H. vulgare. However, what kinds of nucleolar proteins are affected has not been reported. In order to further confirm the cytological effects of Cd on nucleolus and nucleolar proteins, alterations in the cellular localization and expression of two major nucleolar proteins: fibrillarin and nucleolin in the root tips of H. vulgare were studied in the present investigation.
Methods Cytological effects of Cd on nucleolus were observed by silver-staining method and two nucleolarproteins: nucleolin and fibrillarin were observed and analyzed by means of indirect immunofluorescent microscopy and western blotting.
Results Results from the present investigation demonstrated that under Cd stress (cadmium chloride at 50 and 100 ?M), Cd could disturb the nucleolar cycle with the extrusion of silver-stained materials containing argyrophilic proteins from the nucleolus into the cytoplasm. Nucleolar material containing nucleolin and fibrillarin and their movement into the cytoplasm following Cd stress was detected with indirect immunofluorescence techniques. Western blotting revealed higher expression of these two major nucleoproteins in Cd-treated roots, which was consistent with the results obtained by indirect immunofluorescence.
Conclusion The findings of the present study confirm that Cd can have toxic effects on nucleolus and affect the expression of nucleolar proteins, nucleolin and fibrillarin. The data obtained here can provide valuable information for monitoring and forecast early effects of exposure to Cd in polluted environments.
|
| first_indexed | 2024-04-11T16:11:51Z |
| format | Article |
| id | doaj.art-6a9ece38bd524b97b1eb10426b8f2819 |
| institution | Directory Open Access Journal |
| issn | 2007-4298 2007-4476 |
| language | English |
| last_indexed | 2024-04-11T16:11:51Z |
| publishDate | 2017-03-01 |
| publisher | Sociedad Botánica de México, A. C. |
| record_format | Article |
| series | Botanical Sciences |
| spelling | doaj.art-6a9ece38bd524b97b1eb10426b8f28192022-12-22T04:14:40ZengSociedad Botánica de México, A. C.Botanical Sciences2007-42982007-44762017-03-0195110.17129/botsci.694Effects of cadmium on nucleolus in root tips of <em>Hordeum vulgare</em>Qiuyue Shi0Junran Wang1Ze Jiang2Hangfeng Wu3Jiayue Wang4Wusheng Jiang5Donghua Liu6Jinhua Zou7College of Life Sciences, Tianjin Normal University, Tianjin, ChinaCollege of Life Sciences, Tianjin Normal University, Tianjin, ChinaCollege of Life Sciences, Tianjin Normal University, Tianjin, ChinaCollege of Life Sciences, Tianjin Normal University, Tianjin, ChinaCollege of Life Sciences, Tianjin Normal University, Tianjin, ChinaCollege of Life Sciences, Tianjin Normal University, Tianjin, ChinaCollege of Life Sciences, Tianjin Normal University, Tianjin, ChinaCollege of Life Sciences, Tianjin Normal University, Tianjin, China Background Hordeum vulgare is an economically important crop that is consumed in large quantities in the world. In a previous investigation, we found that cadmium (Cd) could induce some nucleolar materials containing the argyrophilic proteins scattered in the nuclei and located in the cytoplasm in the root tip cells of H. vulgare. However, what kinds of nucleolar proteins are affected has not been reported. In order to further confirm the cytological effects of Cd on nucleolus and nucleolar proteins, alterations in the cellular localization and expression of two major nucleolar proteins: fibrillarin and nucleolin in the root tips of H. vulgare were studied in the present investigation. Methods Cytological effects of Cd on nucleolus were observed by silver-staining method and two nucleolarproteins: nucleolin and fibrillarin were observed and analyzed by means of indirect immunofluorescent microscopy and western blotting. Results Results from the present investigation demonstrated that under Cd stress (cadmium chloride at 50 and 100 ?M), Cd could disturb the nucleolar cycle with the extrusion of silver-stained materials containing argyrophilic proteins from the nucleolus into the cytoplasm. Nucleolar material containing nucleolin and fibrillarin and their movement into the cytoplasm following Cd stress was detected with indirect immunofluorescence techniques. Western blotting revealed higher expression of these two major nucleoproteins in Cd-treated roots, which was consistent with the results obtained by indirect immunofluorescence. Conclusion The findings of the present study confirm that Cd can have toxic effects on nucleolus and affect the expression of nucleolar proteins, nucleolin and fibrillarin. The data obtained here can provide valuable information for monitoring and forecast early effects of exposure to Cd in polluted environments. https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/694cadmiumHordeum vulgarenucleolusnucleolinfibrillarinin |
| spellingShingle | Qiuyue Shi Junran Wang Ze Jiang Hangfeng Wu Jiayue Wang Wusheng Jiang Donghua Liu Jinhua Zou Effects of cadmium on nucleolus in root tips of <em>Hordeum vulgare</em> Botanical Sciences cadmium Hordeum vulgare nucleolus nucleolin fibrillarinin |
| title | Effects of cadmium on nucleolus in root tips of <em>Hordeum vulgare</em> |
| title_full | Effects of cadmium on nucleolus in root tips of <em>Hordeum vulgare</em> |
| title_fullStr | Effects of cadmium on nucleolus in root tips of <em>Hordeum vulgare</em> |
| title_full_unstemmed | Effects of cadmium on nucleolus in root tips of <em>Hordeum vulgare</em> |
| title_short | Effects of cadmium on nucleolus in root tips of <em>Hordeum vulgare</em> |
| title_sort | effects of cadmium on nucleolus in root tips of em hordeum vulgare em |
| topic | cadmium Hordeum vulgare nucleolus nucleolin fibrillarinin |
| url | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/694 |
| work_keys_str_mv | AT qiuyueshi effectsofcadmiumonnucleolusinroottipsofemhordeumvulgareem AT junranwang effectsofcadmiumonnucleolusinroottipsofemhordeumvulgareem AT zejiang effectsofcadmiumonnucleolusinroottipsofemhordeumvulgareem AT hangfengwu effectsofcadmiumonnucleolusinroottipsofemhordeumvulgareem AT jiayuewang effectsofcadmiumonnucleolusinroottipsofemhordeumvulgareem AT wushengjiang effectsofcadmiumonnucleolusinroottipsofemhordeumvulgareem AT donghualiu effectsofcadmiumonnucleolusinroottipsofemhordeumvulgareem AT jinhuazou effectsofcadmiumonnucleolusinroottipsofemhordeumvulgareem |