OsMKK6 Regulates Disease Resistance in Rice
Mitogen-activated protein kinase cascades play important roles in various biological programs in plants, including immune responses, but the underlying mechanisms remain elusive. Here, we identified the lesion mimic mutant <i>rsr25</i> (<i>rust spots rice 25</i>) and determin...
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/16/12678 |
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| author | Ruirui Jiang Shichen Zhou Xiaowen Da Peng Yan Kai Wang Jiming Xu Xiaorong Mo |
| author_facet | Ruirui Jiang Shichen Zhou Xiaowen Da Peng Yan Kai Wang Jiming Xu Xiaorong Mo |
| author_sort | Ruirui Jiang |
| collection | DOAJ |
| description | Mitogen-activated protein kinase cascades play important roles in various biological programs in plants, including immune responses, but the underlying mechanisms remain elusive. Here, we identified the lesion mimic mutant <i>rsr25</i> (<i>rust spots rice 25</i>) and determined that the mutant harbored a loss-of-function allele for <i>OsMKK6 (MITOGEN-ACTIVATED KINASE KINASE 6)</i>. <i>rsr25</i> developed reddish-brown spots on its leaves at the heading stage, as well as on husks. Compared to the wild type, the <i>rsr25</i> mutant exhibited enhanced resistance to the fungal pathogen <i>Magnaporthe oryzae</i> (<i>M. oryzae</i>) and to the bacterial pathogen <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>). OsMKK6 interacted with OsMPK4 (MITOGEN-ACTIVATED KINASE 4) in vivo, and OsMKK6 phosphorylated OsMPK4 in vitro. The <i>Osmpk4</i> mutant is also a lesion mimic mutant, with reddish-brown spots on its leaves and husks. Pathogen-related genes were significantly upregulated in <i>Osmpk4</i>, and this mutant exhibited enhanced resistance to <i>M. oryzae</i> compared to the wild type. Our results indicate that OsMKK6 and OsMPK4 form a cascade that regulates immune responses in rice. |
| first_indexed | 2024-03-10T23:52:22Z |
| format | Article |
| id | doaj.art-6aa1a38167a84012ba33edbcae164a44 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:52:22Z |
| publishDate | 2023-08-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6aa1a38167a84012ba33edbcae164a442023-11-19T01:27:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124161267810.3390/ijms241612678OsMKK6 Regulates Disease Resistance in RiceRuirui Jiang0Shichen Zhou1Xiaowen Da2Peng Yan3Kai Wang4Jiming Xu5Xiaorong Mo6State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, ChinaMitogen-activated protein kinase cascades play important roles in various biological programs in plants, including immune responses, but the underlying mechanisms remain elusive. Here, we identified the lesion mimic mutant <i>rsr25</i> (<i>rust spots rice 25</i>) and determined that the mutant harbored a loss-of-function allele for <i>OsMKK6 (MITOGEN-ACTIVATED KINASE KINASE 6)</i>. <i>rsr25</i> developed reddish-brown spots on its leaves at the heading stage, as well as on husks. Compared to the wild type, the <i>rsr25</i> mutant exhibited enhanced resistance to the fungal pathogen <i>Magnaporthe oryzae</i> (<i>M. oryzae</i>) and to the bacterial pathogen <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>). OsMKK6 interacted with OsMPK4 (MITOGEN-ACTIVATED KINASE 4) in vivo, and OsMKK6 phosphorylated OsMPK4 in vitro. The <i>Osmpk4</i> mutant is also a lesion mimic mutant, with reddish-brown spots on its leaves and husks. Pathogen-related genes were significantly upregulated in <i>Osmpk4</i>, and this mutant exhibited enhanced resistance to <i>M. oryzae</i> compared to the wild type. Our results indicate that OsMKK6 and OsMPK4 form a cascade that regulates immune responses in rice.https://www.mdpi.com/1422-0067/24/16/12678rice (<i>Oryza sativa</i> L.)lesion mimic mutantdefense responseOsMKK6 |
| spellingShingle | Ruirui Jiang Shichen Zhou Xiaowen Da Peng Yan Kai Wang Jiming Xu Xiaorong Mo OsMKK6 Regulates Disease Resistance in Rice International Journal of Molecular Sciences rice (<i>Oryza sativa</i> L.) lesion mimic mutant defense response OsMKK6 |
| title | OsMKK6 Regulates Disease Resistance in Rice |
| title_full | OsMKK6 Regulates Disease Resistance in Rice |
| title_fullStr | OsMKK6 Regulates Disease Resistance in Rice |
| title_full_unstemmed | OsMKK6 Regulates Disease Resistance in Rice |
| title_short | OsMKK6 Regulates Disease Resistance in Rice |
| title_sort | osmkk6 regulates disease resistance in rice |
| topic | rice (<i>Oryza sativa</i> L.) lesion mimic mutant defense response OsMKK6 |
| url | https://www.mdpi.com/1422-0067/24/16/12678 |
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