Quantitative HPLC–UV Study of Lignans in <i>Anthriscus sylvestris</i>
Wild chervil (<i>Anthriscus sylvestris</i>) is a wild-growing plant from the Apiaceae family, used as a food in Europe and eastern Asia. Due to its high content of lignans known to possess anti-inflammatory, antiproliferative, antiviral and other activities, it represents a potential fun...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/18/6072 |
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| author | Dejan Orčić Sanja Berežni Neda Mimica-Dukić |
| author_facet | Dejan Orčić Sanja Berežni Neda Mimica-Dukić |
| author_sort | Dejan Orčić |
| collection | DOAJ |
| description | Wild chervil (<i>Anthriscus sylvestris</i>) is a wild-growing plant from the Apiaceae family, used as a food in Europe and eastern Asia. Due to its high content of lignans known to possess anti-inflammatory, antiproliferative, antiviral and other activities, it represents a potential functional food. However, quantitative data on lignans are still scarce and limited to deoxypodophyllotoxin, nemerosin and yatein. In this paper, a newly developed and validated reverse-phase HPLC–UV method was used to evaluate the content of 14 lignans in both aerial parts and roots of <i>A. sylvestris</i>. The most abundant root components were found to be deoxypodophyllotoxin (2.0–42.8 mg/g), nemerosin (2.0–23.4 mg/g), yatein (1.1–18.5 mg/g), podophyllotoxone (0.7–20.5 mg/g), guaiadequiol (0.8–8.3 mg/g) and dimethylmatairesinol (0.1–5.2 mg/g). Despite the high intra-population variability, a general trend of an increased lignan content during plant development could be observed in the root samples, whereas an opposite trend was observed in the herb samples. A validation study indicated that some of the investigated compounds—7-oxoaryltetralins and dibenzylbutyrolactones—have low stability and require cold storage in the dark. Furthermore, dibenzylbutyrolactones were confirmed to undergo a fast <i>cis</i>–<i>trans</i> isomerization; therefore, only the total content of these isomers should be reported. |
| first_indexed | 2024-03-09T23:00:03Z |
| format | Article |
| id | doaj.art-6aa35f749d6341738fd4675347e8fc04 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:00:03Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6aa35f749d6341738fd4675347e8fc042023-11-23T18:03:48ZengMDPI AGMolecules1420-30492022-09-012718607210.3390/molecules27186072Quantitative HPLC–UV Study of Lignans in <i>Anthriscus sylvestris</i>Dejan Orčić0Sanja Berežni1Neda Mimica-Dukić2Faculty of Sciences, University of Novi Sad, 21102 Novi Sad, SerbiaFaculty of Sciences, University of Novi Sad, 21102 Novi Sad, SerbiaFaculty of Sciences, University of Novi Sad, 21102 Novi Sad, SerbiaWild chervil (<i>Anthriscus sylvestris</i>) is a wild-growing plant from the Apiaceae family, used as a food in Europe and eastern Asia. Due to its high content of lignans known to possess anti-inflammatory, antiproliferative, antiviral and other activities, it represents a potential functional food. However, quantitative data on lignans are still scarce and limited to deoxypodophyllotoxin, nemerosin and yatein. In this paper, a newly developed and validated reverse-phase HPLC–UV method was used to evaluate the content of 14 lignans in both aerial parts and roots of <i>A. sylvestris</i>. The most abundant root components were found to be deoxypodophyllotoxin (2.0–42.8 mg/g), nemerosin (2.0–23.4 mg/g), yatein (1.1–18.5 mg/g), podophyllotoxone (0.7–20.5 mg/g), guaiadequiol (0.8–8.3 mg/g) and dimethylmatairesinol (0.1–5.2 mg/g). Despite the high intra-population variability, a general trend of an increased lignan content during plant development could be observed in the root samples, whereas an opposite trend was observed in the herb samples. A validation study indicated that some of the investigated compounds—7-oxoaryltetralins and dibenzylbutyrolactones—have low stability and require cold storage in the dark. Furthermore, dibenzylbutyrolactones were confirmed to undergo a fast <i>cis</i>–<i>trans</i> isomerization; therefore, only the total content of these isomers should be reported.https://www.mdpi.com/1420-3049/27/18/6072<i>Anthriscus sylvestris</i>ApiaceaequantificationvalidationHPLClignans |
| spellingShingle | Dejan Orčić Sanja Berežni Neda Mimica-Dukić Quantitative HPLC–UV Study of Lignans in <i>Anthriscus sylvestris</i> Molecules <i>Anthriscus sylvestris</i> Apiaceae quantification validation HPLC lignans |
| title | Quantitative HPLC–UV Study of Lignans in <i>Anthriscus sylvestris</i> |
| title_full | Quantitative HPLC–UV Study of Lignans in <i>Anthriscus sylvestris</i> |
| title_fullStr | Quantitative HPLC–UV Study of Lignans in <i>Anthriscus sylvestris</i> |
| title_full_unstemmed | Quantitative HPLC–UV Study of Lignans in <i>Anthriscus sylvestris</i> |
| title_short | Quantitative HPLC–UV Study of Lignans in <i>Anthriscus sylvestris</i> |
| title_sort | quantitative hplc uv study of lignans in i anthriscus sylvestris i |
| topic | <i>Anthriscus sylvestris</i> Apiaceae quantification validation HPLC lignans |
| url | https://www.mdpi.com/1420-3049/27/18/6072 |
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