Morphological Evaluation of Transscleral Laser Retinopexy in Rabbits: Comparison of Optical Coherence Tomography and Histologic Examinations
Transscleral retinopexy is a preventive technique used against retinal detachment. Fundus examination can allow the monitoring of morphological retinal changes in the progression of photocoagulation lesions, without offering details on the morphological changes by the retinal lesion. The aim of the...
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MDPI AG
2023-08-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/9/535 |
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| author | Maria Vanore Tristan Juette Javier Benito Marie-Odile Benoit-Biancamano |
| author_facet | Maria Vanore Tristan Juette Javier Benito Marie-Odile Benoit-Biancamano |
| author_sort | Maria Vanore |
| collection | DOAJ |
| description | Transscleral retinopexy is a preventive technique used against retinal detachment. Fundus examination can allow the monitoring of morphological retinal changes in the progression of photocoagulation lesions, without offering details on the morphological changes by the retinal lesion. The aim of the study was to assess the progression of photocoagulation lesions induced by transscleral retinopexy (840 nm diode laser), by comparing the optical coherence tomography (OCT) and histological images over a period of six weeks on eight pigmented New Zealand healthy rabbits (four males and four females; n = 16 eyes)<b>.</b> All rabbits underwent transscleral retinopexy on their left eye on day 0 (D0). Measurements of the photocoagulation lesions were obtained in vivo on D0, D7, D15, D21, and D42 by acquiring OCT images of both eyes from all rabbits. On D1, D7, D21, and D42, two rabbits were euthanized, and their eyes were enucleated. A significant effect by time on the decrease in the central retinal thickness of the photocoagulation lesion was observed from D1 to D7 (<i>p</i> = 0.001); however, no such effect was observed on the horizontal length ((HL) <i>p</i> = 0.584) of the lesion surface. The reliability between the OCT and histological measurements, which were evaluated using intraclass correlation coefficients, was excellent for measuring the retinal thickness at the center (ICC = 0.91, <i>p</i> < 0.001), moderate for the right side of the retinal lesions (ICC = 0.72, <i>p</i> = 0.006), and not significant for the left side and HL (<i>p</i> = 0.055 and 0.500, respectively). The morphological changes observed in the OCT and histopathological images of the photocoagulation lesions were qualitatively described over time. OCT is an effective tool for monitoring changes in photocoagulation lesions. Some measurements and qualitative changes showed an adequate correlation between the OCT and histological findings. |
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| language | English |
| last_indexed | 2024-03-10T21:52:33Z |
| publishDate | 2023-08-01 |
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| series | Veterinary Sciences |
| spelling | doaj.art-6aa5b024ab12431086657141bfe608f32023-11-19T13:20:30ZengMDPI AGVeterinary Sciences2306-73812023-08-0110953510.3390/vetsci10090535Morphological Evaluation of Transscleral Laser Retinopexy in Rabbits: Comparison of Optical Coherence Tomography and Histologic ExaminationsMaria Vanore0Tristan Juette1Javier Benito2Marie-Odile Benoit-Biancamano3Centre Hospitalier Universitaire Vétérinaire, Département de Sciences Cliniques, Faculté de Médecine Vétérinaire, Université de Montréal, 3200 Rue Sicotte, Saint-Hyacinthe, QC J2S 2M2, CanadaFaculté de Médecine Vétérinaire, Université de Montréal, 3200 Rue Sicotte, Saint-Hyacinthe, QC J2S 2M2, CanadaCentre Hospitalier Universitaire Vétérinaire, Département de Sciences Cliniques, Faculté de Médecine Vétérinaire, Université de Montréal, 3200 Rue Sicotte, Saint-Hyacinthe, QC J2S 2M2, CanadaGroupe de Recherche sur les Maladies Infectieuses en Production Animale (GREMIP), Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, 3200 Rue Sicotte, Saint-Hyacinthe, QC J2S 2M2, CanadaTransscleral retinopexy is a preventive technique used against retinal detachment. Fundus examination can allow the monitoring of morphological retinal changes in the progression of photocoagulation lesions, without offering details on the morphological changes by the retinal lesion. The aim of the study was to assess the progression of photocoagulation lesions induced by transscleral retinopexy (840 nm diode laser), by comparing the optical coherence tomography (OCT) and histological images over a period of six weeks on eight pigmented New Zealand healthy rabbits (four males and four females; n = 16 eyes)<b>.</b> All rabbits underwent transscleral retinopexy on their left eye on day 0 (D0). Measurements of the photocoagulation lesions were obtained in vivo on D0, D7, D15, D21, and D42 by acquiring OCT images of both eyes from all rabbits. On D1, D7, D21, and D42, two rabbits were euthanized, and their eyes were enucleated. A significant effect by time on the decrease in the central retinal thickness of the photocoagulation lesion was observed from D1 to D7 (<i>p</i> = 0.001); however, no such effect was observed on the horizontal length ((HL) <i>p</i> = 0.584) of the lesion surface. The reliability between the OCT and histological measurements, which were evaluated using intraclass correlation coefficients, was excellent for measuring the retinal thickness at the center (ICC = 0.91, <i>p</i> < 0.001), moderate for the right side of the retinal lesions (ICC = 0.72, <i>p</i> = 0.006), and not significant for the left side and HL (<i>p</i> = 0.055 and 0.500, respectively). The morphological changes observed in the OCT and histopathological images of the photocoagulation lesions were qualitatively described over time. OCT is an effective tool for monitoring changes in photocoagulation lesions. Some measurements and qualitative changes showed an adequate correlation between the OCT and histological findings.https://www.mdpi.com/2306-7381/10/9/535diode laserhistologyoptical coherence tomographyphotocoagulationrabbitstransscleral retinopexy |
| spellingShingle | Maria Vanore Tristan Juette Javier Benito Marie-Odile Benoit-Biancamano Morphological Evaluation of Transscleral Laser Retinopexy in Rabbits: Comparison of Optical Coherence Tomography and Histologic Examinations Veterinary Sciences diode laser histology optical coherence tomography photocoagulation rabbits transscleral retinopexy |
| title | Morphological Evaluation of Transscleral Laser Retinopexy in Rabbits: Comparison of Optical Coherence Tomography and Histologic Examinations |
| title_full | Morphological Evaluation of Transscleral Laser Retinopexy in Rabbits: Comparison of Optical Coherence Tomography and Histologic Examinations |
| title_fullStr | Morphological Evaluation of Transscleral Laser Retinopexy in Rabbits: Comparison of Optical Coherence Tomography and Histologic Examinations |
| title_full_unstemmed | Morphological Evaluation of Transscleral Laser Retinopexy in Rabbits: Comparison of Optical Coherence Tomography and Histologic Examinations |
| title_short | Morphological Evaluation of Transscleral Laser Retinopexy in Rabbits: Comparison of Optical Coherence Tomography and Histologic Examinations |
| title_sort | morphological evaluation of transscleral laser retinopexy in rabbits comparison of optical coherence tomography and histologic examinations |
| topic | diode laser histology optical coherence tomography photocoagulation rabbits transscleral retinopexy |
| url | https://www.mdpi.com/2306-7381/10/9/535 |
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