Comparative immunohistochemical analysis of inflammatory cytokines in distinct subtypes of Sweet syndrome
BackgroundA dysregulated immune response has been implicated in Sweet syndrome (SS) pathogenesis; however, cytokine profiles across different conditions associated with SS — including adult-onset immunodeficiency (AOID) due to anti-interferon (IFN)-γ autoantibodies — remain unknown.ObjectiveTo inves...
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| Format: | Article |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1355681/full |
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| author | Panjit Chieosilapatham Panjit Chieosilapatham Teerada Daroontum Songkiet Suwansirikul Romanee Chaiwarith Phichayut Phinyo Phichayut Phinyo Suteeraporn Chaowattanapanit Charoen Choonhakarn Salin Kiratikanon Rujira Rujiwetpongstorn Napatra Tovanabutra Siri Chiewchanvit Mati Chuamanochan Mati Chuamanochan |
| author_facet | Panjit Chieosilapatham Panjit Chieosilapatham Teerada Daroontum Songkiet Suwansirikul Romanee Chaiwarith Phichayut Phinyo Phichayut Phinyo Suteeraporn Chaowattanapanit Charoen Choonhakarn Salin Kiratikanon Rujira Rujiwetpongstorn Napatra Tovanabutra Siri Chiewchanvit Mati Chuamanochan Mati Chuamanochan |
| author_sort | Panjit Chieosilapatham |
| collection | DOAJ |
| description | BackgroundA dysregulated immune response has been implicated in Sweet syndrome (SS) pathogenesis; however, cytokine profiles across different conditions associated with SS — including adult-onset immunodeficiency (AOID) due to anti-interferon (IFN)-γ autoantibodies — remain unknown.ObjectiveTo investigate alterations in inflammatory cytokines in skin lesions of distinct subtypes of SS.MethodsSkin biopsies were collected from 42 AOID- and 52 non-AOID-associated SS patients and 18 healthy controls. The comparative immunohistochemical study was conducted using monoclonal antibodies against interleukin (IL)-1β, IL-6, IL-17, IFN-γ, and tumor necrosis factor-α on paraffin-embedded sections. The quantitative percentage positivity and intensity were calculated using computer-based image analysis.ResultsThe results showed stronger and more diffuse dermal immunoreactivity for IFN-γ and IL-17 in the AOID-associated (p < 0.001 and p < 0.001, respectively) and non-AOID-associated SS (p < 0.001 and p < 0.001, respectively) groups. However, no significant differences in the levels of these two cytokines were observed between the AOID- and non-AOID-associated SS groups. Increased expression of IFN-γ together with IL-17 was also noted in almost all subtypes among non-AOID-associated SS.ConclusionsThese results demonstrate that IFN-γ and IL-17 are implicated in immunopathology of all SS subtypes, including AOID-associated SS, despite the presence of anti-IFN-γ autoantibodies. |
| first_indexed | 2024-04-25T01:00:31Z |
| format | Article |
| id | doaj.art-6aab39a933054b28924fdbbb0920f4fc |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-25T01:00:31Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-6aab39a933054b28924fdbbb0920f4fc2024-03-11T04:45:20ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-03-011510.3389/fimmu.2024.13556811355681Comparative immunohistochemical analysis of inflammatory cytokines in distinct subtypes of Sweet syndromePanjit Chieosilapatham0Panjit Chieosilapatham1Teerada Daroontum2Songkiet Suwansirikul3Romanee Chaiwarith4Phichayut Phinyo5Phichayut Phinyo6Suteeraporn Chaowattanapanit7Charoen Choonhakarn8Salin Kiratikanon9Rujira Rujiwetpongstorn10Napatra Tovanabutra11Siri Chiewchanvit12Mati Chuamanochan13Mati Chuamanochan14Division of Dermatology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDivision of Immunology, Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDepartment of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDepartment of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDivision of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandCenter for Clinical Epidemiology and Clinical Statistics, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDepartment of Family Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDivision of Dermatology, Department of Medicine, Faculty of Medicine, Srinagarind Hospital, Khon Kaen University, Khon Kaen, ThailandDivision of Dermatology, Department of Medicine, Faculty of Medicine, Srinagarind Hospital, Khon Kaen University, Khon Kaen, ThailandDivision of Dermatology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDivision of Dermatology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDivision of Dermatology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDivision of Dermatology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandDivision of Dermatology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, ThailandPharmacoepidemiology and Statistics Research Center (PESRC), Faculty of Pharmacy, Chiang Mai University, Chiang Mai, ThailandBackgroundA dysregulated immune response has been implicated in Sweet syndrome (SS) pathogenesis; however, cytokine profiles across different conditions associated with SS — including adult-onset immunodeficiency (AOID) due to anti-interferon (IFN)-γ autoantibodies — remain unknown.ObjectiveTo investigate alterations in inflammatory cytokines in skin lesions of distinct subtypes of SS.MethodsSkin biopsies were collected from 42 AOID- and 52 non-AOID-associated SS patients and 18 healthy controls. The comparative immunohistochemical study was conducted using monoclonal antibodies against interleukin (IL)-1β, IL-6, IL-17, IFN-γ, and tumor necrosis factor-α on paraffin-embedded sections. The quantitative percentage positivity and intensity were calculated using computer-based image analysis.ResultsThe results showed stronger and more diffuse dermal immunoreactivity for IFN-γ and IL-17 in the AOID-associated (p < 0.001 and p < 0.001, respectively) and non-AOID-associated SS (p < 0.001 and p < 0.001, respectively) groups. However, no significant differences in the levels of these two cytokines were observed between the AOID- and non-AOID-associated SS groups. Increased expression of IFN-γ together with IL-17 was also noted in almost all subtypes among non-AOID-associated SS.ConclusionsThese results demonstrate that IFN-γ and IL-17 are implicated in immunopathology of all SS subtypes, including AOID-associated SS, despite the presence of anti-IFN-γ autoantibodies.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1355681/fulladult-onset immunodeficiencyanti-IFN-γ autoantibodycytokine expressionimmunohistochemical stainingsweet syndrome |
| spellingShingle | Panjit Chieosilapatham Panjit Chieosilapatham Teerada Daroontum Songkiet Suwansirikul Romanee Chaiwarith Phichayut Phinyo Phichayut Phinyo Suteeraporn Chaowattanapanit Charoen Choonhakarn Salin Kiratikanon Rujira Rujiwetpongstorn Napatra Tovanabutra Siri Chiewchanvit Mati Chuamanochan Mati Chuamanochan Comparative immunohistochemical analysis of inflammatory cytokines in distinct subtypes of Sweet syndrome Frontiers in Immunology adult-onset immunodeficiency anti-IFN-γ autoantibody cytokine expression immunohistochemical staining sweet syndrome |
| title | Comparative immunohistochemical analysis of inflammatory cytokines in distinct subtypes of Sweet syndrome |
| title_full | Comparative immunohistochemical analysis of inflammatory cytokines in distinct subtypes of Sweet syndrome |
| title_fullStr | Comparative immunohistochemical analysis of inflammatory cytokines in distinct subtypes of Sweet syndrome |
| title_full_unstemmed | Comparative immunohistochemical analysis of inflammatory cytokines in distinct subtypes of Sweet syndrome |
| title_short | Comparative immunohistochemical analysis of inflammatory cytokines in distinct subtypes of Sweet syndrome |
| title_sort | comparative immunohistochemical analysis of inflammatory cytokines in distinct subtypes of sweet syndrome |
| topic | adult-onset immunodeficiency anti-IFN-γ autoantibody cytokine expression immunohistochemical staining sweet syndrome |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1355681/full |
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