Immunoinformatics-guided approach for designing a pan-proteome multi-epitope subunit vaccine against African swine fever virus
Abstract Despite being identified over a hundred years ago, there is still no commercially available vaccine for the highly contagious and deadly African swine fever virus (ASFV). This study used immunoinformatics for the rapid and inexpensive designing of a safe and effective multi-epitope subunit...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-01-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-51005-3 |
| _version_ | 1797349930052878336 |
|---|---|
| author | Alea Maurice Simbulan Edward C. Banico Ella Mae Joy S. Sira Nyzar Mabeth O. Odchimar Fredmoore L. Orosco |
| author_facet | Alea Maurice Simbulan Edward C. Banico Ella Mae Joy S. Sira Nyzar Mabeth O. Odchimar Fredmoore L. Orosco |
| author_sort | Alea Maurice Simbulan |
| collection | DOAJ |
| description | Abstract Despite being identified over a hundred years ago, there is still no commercially available vaccine for the highly contagious and deadly African swine fever virus (ASFV). This study used immunoinformatics for the rapid and inexpensive designing of a safe and effective multi-epitope subunit vaccine for ASFV. A total of 18,858 proteins from 100 well-annotated ASFV proteomes were screened using various computational tools to identify potential epitopes, or peptides capable of triggering an immune response in swine. Proteins from genotypes I and II were prioritized for their involvement in the recent global ASFV outbreaks. The screened epitopes exhibited promising qualities that positioned them as effective components of the ASFV vaccine. They demonstrated antigenicity, immunogenicity, and cytokine-inducing properties indicating their ability to induce potent immune responses. They have strong binding affinities to multiple swine allele receptors suggesting a high likelihood of yielding more amplified responses. Moreover, they were non-allergenic and non-toxic, a crucial prerequisite for ensuring safety and minimizing any potential adverse effects when the vaccine is processed within the host. Integrated with an immunogenic 50S ribosomal protein adjuvant and linkers, the epitopes formed a 364-amino acid multi-epitope subunit vaccine. The ASFV vaccine construct exhibited notable immunogenicity in immune simulation and molecular docking analyses, and stable profiles in secondary and tertiary structure assessments. Moreover, this study designed an optimized codon for efficient translation of the ASFV vaccine construct into the Escherichia coli K-12 expression system using the pET28a(+) vector. Overall, both sequence and structural evaluations suggested the potential of the ASFV vaccine construct as a candidate for controlling and eradicating outbreaks caused by the pathogen. |
| first_indexed | 2024-03-08T12:38:27Z |
| format | Article |
| id | doaj.art-6aab4accb0394ca68f3677a3d4bc34ee |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T12:38:27Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6aab4accb0394ca68f3677a3d4bc34ee2024-01-21T12:20:13ZengNature PortfolioScientific Reports2045-23222024-01-0114111410.1038/s41598-023-51005-3Immunoinformatics-guided approach for designing a pan-proteome multi-epitope subunit vaccine against African swine fever virusAlea Maurice Simbulan0Edward C. Banico1Ella Mae Joy S. Sira2Nyzar Mabeth O. Odchimar3Fredmoore L. Orosco4Department of Science and Technology, Virology and Vaccine Research and Development Program, Industrial Technology Development InstituteDepartment of Science and Technology, Virology and Vaccine Research and Development Program, Industrial Technology Development InstituteDepartment of Science and Technology, Virology and Vaccine Research and Development Program, Industrial Technology Development InstituteDepartment of Science and Technology, Virology and Vaccine Research and Development Program, Industrial Technology Development InstituteDepartment of Science and Technology, Virology and Vaccine Research and Development Program, Industrial Technology Development InstituteAbstract Despite being identified over a hundred years ago, there is still no commercially available vaccine for the highly contagious and deadly African swine fever virus (ASFV). This study used immunoinformatics for the rapid and inexpensive designing of a safe and effective multi-epitope subunit vaccine for ASFV. A total of 18,858 proteins from 100 well-annotated ASFV proteomes were screened using various computational tools to identify potential epitopes, or peptides capable of triggering an immune response in swine. Proteins from genotypes I and II were prioritized for their involvement in the recent global ASFV outbreaks. The screened epitopes exhibited promising qualities that positioned them as effective components of the ASFV vaccine. They demonstrated antigenicity, immunogenicity, and cytokine-inducing properties indicating their ability to induce potent immune responses. They have strong binding affinities to multiple swine allele receptors suggesting a high likelihood of yielding more amplified responses. Moreover, they were non-allergenic and non-toxic, a crucial prerequisite for ensuring safety and minimizing any potential adverse effects when the vaccine is processed within the host. Integrated with an immunogenic 50S ribosomal protein adjuvant and linkers, the epitopes formed a 364-amino acid multi-epitope subunit vaccine. The ASFV vaccine construct exhibited notable immunogenicity in immune simulation and molecular docking analyses, and stable profiles in secondary and tertiary structure assessments. Moreover, this study designed an optimized codon for efficient translation of the ASFV vaccine construct into the Escherichia coli K-12 expression system using the pET28a(+) vector. Overall, both sequence and structural evaluations suggested the potential of the ASFV vaccine construct as a candidate for controlling and eradicating outbreaks caused by the pathogen.https://doi.org/10.1038/s41598-023-51005-3 |
| spellingShingle | Alea Maurice Simbulan Edward C. Banico Ella Mae Joy S. Sira Nyzar Mabeth O. Odchimar Fredmoore L. Orosco Immunoinformatics-guided approach for designing a pan-proteome multi-epitope subunit vaccine against African swine fever virus Scientific Reports |
| title | Immunoinformatics-guided approach for designing a pan-proteome multi-epitope subunit vaccine against African swine fever virus |
| title_full | Immunoinformatics-guided approach for designing a pan-proteome multi-epitope subunit vaccine against African swine fever virus |
| title_fullStr | Immunoinformatics-guided approach for designing a pan-proteome multi-epitope subunit vaccine against African swine fever virus |
| title_full_unstemmed | Immunoinformatics-guided approach for designing a pan-proteome multi-epitope subunit vaccine against African swine fever virus |
| title_short | Immunoinformatics-guided approach for designing a pan-proteome multi-epitope subunit vaccine against African swine fever virus |
| title_sort | immunoinformatics guided approach for designing a pan proteome multi epitope subunit vaccine against african swine fever virus |
| url | https://doi.org/10.1038/s41598-023-51005-3 |
| work_keys_str_mv | AT aleamauricesimbulan immunoinformaticsguidedapproachfordesigningapanproteomemultiepitopesubunitvaccineagainstafricanswinefevervirus AT edwardcbanico immunoinformaticsguidedapproachfordesigningapanproteomemultiepitopesubunitvaccineagainstafricanswinefevervirus AT ellamaejoyssira immunoinformaticsguidedapproachfordesigningapanproteomemultiepitopesubunitvaccineagainstafricanswinefevervirus AT nyzarmabethoodchimar immunoinformaticsguidedapproachfordesigningapanproteomemultiepitopesubunitvaccineagainstafricanswinefevervirus AT fredmoorelorosco immunoinformaticsguidedapproachfordesigningapanproteomemultiepitopesubunitvaccineagainstafricanswinefevervirus |