Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats
Background: Osteoarthritis is the most common degenerative joint disease worldwide but its treatment can cause serious adverse events. Ethanol extract of peanut shells contains luteolin functioned as its main anti-inflammatory and chondroprotective agent. Apis dorsata honey also contains anti-inflam...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Diponegoro University
2020-12-01
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| Series: | Journal of Biomedicine and Translational Research |
| Subjects: | |
| Online Access: | https://ejournal2.undip.ac.id/index.php/jbtr/article/view/7908 |
| _version_ | 1818900221703225344 |
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| author | Bella Stevanny Tungki Pratama Umar Junoretta Haviva Ernanto Rachmat Hidayat |
| author_facet | Bella Stevanny Tungki Pratama Umar Junoretta Haviva Ernanto Rachmat Hidayat |
| author_sort | Bella Stevanny |
| collection | DOAJ |
| description | Background: Osteoarthritis is the most common degenerative joint disease worldwide but its treatment can cause serious adverse events. Ethanol extract of peanut shells contains luteolin functioned as its main anti-inflammatory and chondroprotective agent. Apis dorsata honey also contains anti-inflammatory and antioxidant agent, especially from Tualang honey type. Both have the potential to reduce inflammation and prevent articular degradation in osteoarthritis.
Objective: This study aims to determine the effect of peanut shells extract and Apis dorsata honey on matrix metalloproteinase-3 (MMP-3) level in monosodium iodoacetate-induced (MIA) osteoarthritic rats.
Methods: In this in vivo study, female Wistar rats (n=27) were randomly divided to nine groups containing three rats each. Treatment was given to group 1, 2, and 3: Tualang honey (TH) 25% + peanut shells extract (PSE) with 1%, 5%, and 10% concentration; group 4, 5, and 6: TH 50% + PSE with 1%, 5%, and 10% concentration; group 7: diclofenac sodium (positive control); group 8: aquadest (negative control); and group 9: aquadest (normal) for 10 days. We induced knee osteoarthritis by intraarticular injection of MIA in day 4. Anti-inflammatory and chondroprotective activities were evaluated with MMP-3 ELISA.
Results: The mixture of peanut shells extract and Apis dorsata honey significantly reduced MMP-3 level in group 1 (331.12 pg/ml), group 2 (291.73 pg/ml), group 3 (266.58 pg/ml), group 4 (274.15 pg/ml), group 5 (251.12 pg/ml), and group 6 (220.52 pg/ml) after 10 days of treatment. MMP-3 level was also evaluated in group 7 (169.61 pg/ml), group 8 (413.55 pg/ml), and group 9 (39 pg/ml). Compared to the negative control group, treatment and diclofenac groups showed significant effect in reducing MMP-3 level in patello-femoral articular cartilage.
Conclusion: Peanut shells extract and Apis dorsata honey showed antiinflammatory and chondroprotective effect by reducing MMP-3 level in MIA-induced osteoarthritic rats. |
| first_indexed | 2024-12-19T20:00:25Z |
| format | Article |
| id | doaj.art-6aac35ac89c64a93ad8b18c47b54a363 |
| institution | Directory Open Access Journal |
| issn | 2503-2178 |
| language | English |
| last_indexed | 2024-12-19T20:00:25Z |
| publishDate | 2020-12-01 |
| publisher | Diponegoro University |
| record_format | Article |
| series | Journal of Biomedicine and Translational Research |
| spelling | doaj.art-6aac35ac89c64a93ad8b18c47b54a3632022-12-21T20:07:41ZengDiponegoro UniversityJournal of Biomedicine and Translational Research2503-21782020-12-0163747810.14710/jbtr.v6i3.79084536Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic RatsBella Stevanny0https://orcid.org/0000-0002-5135-3012Tungki Pratama Umar1https://orcid.org/0000-0001-6975-8096Junoretta Haviva Ernanto2https://orcid.org/0000-0002-5063-4891Rachmat Hidayat3https://orcid.org/0000-0001-5770-1201Sriwijaya University, IndonesiaSriwijaya University, IndonesiaSriwijaya University, IndonesiaSriwijaya University, IndonesiaBackground: Osteoarthritis is the most common degenerative joint disease worldwide but its treatment can cause serious adverse events. Ethanol extract of peanut shells contains luteolin functioned as its main anti-inflammatory and chondroprotective agent. Apis dorsata honey also contains anti-inflammatory and antioxidant agent, especially from Tualang honey type. Both have the potential to reduce inflammation and prevent articular degradation in osteoarthritis. Objective: This study aims to determine the effect of peanut shells extract and Apis dorsata honey on matrix metalloproteinase-3 (MMP-3) level in monosodium iodoacetate-induced (MIA) osteoarthritic rats. Methods: In this in vivo study, female Wistar rats (n=27) were randomly divided to nine groups containing three rats each. Treatment was given to group 1, 2, and 3: Tualang honey (TH) 25% + peanut shells extract (PSE) with 1%, 5%, and 10% concentration; group 4, 5, and 6: TH 50% + PSE with 1%, 5%, and 10% concentration; group 7: diclofenac sodium (positive control); group 8: aquadest (negative control); and group 9: aquadest (normal) for 10 days. We induced knee osteoarthritis by intraarticular injection of MIA in day 4. Anti-inflammatory and chondroprotective activities were evaluated with MMP-3 ELISA. Results: The mixture of peanut shells extract and Apis dorsata honey significantly reduced MMP-3 level in group 1 (331.12 pg/ml), group 2 (291.73 pg/ml), group 3 (266.58 pg/ml), group 4 (274.15 pg/ml), group 5 (251.12 pg/ml), and group 6 (220.52 pg/ml) after 10 days of treatment. MMP-3 level was also evaluated in group 7 (169.61 pg/ml), group 8 (413.55 pg/ml), and group 9 (39 pg/ml). Compared to the negative control group, treatment and diclofenac groups showed significant effect in reducing MMP-3 level in patello-femoral articular cartilage. Conclusion: Peanut shells extract and Apis dorsata honey showed antiinflammatory and chondroprotective effect by reducing MMP-3 level in MIA-induced osteoarthritic rats.https://ejournal2.undip.ac.id/index.php/jbtr/article/view/7908apis dorsata honeyinflammationmmp-3osteoarthritispeanut shells extract |
| spellingShingle | Bella Stevanny Tungki Pratama Umar Junoretta Haviva Ernanto Rachmat Hidayat Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats Journal of Biomedicine and Translational Research apis dorsata honey inflammation mmp-3 osteoarthritis peanut shells extract |
| title | Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats |
| title_full | Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats |
| title_fullStr | Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats |
| title_full_unstemmed | Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats |
| title_short | Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats |
| title_sort | peanut arachis hypogaea shells extract and apis dorsata honey reduce matrix metalloproteinase 3 in monosodium iodoacetate induced osteoarthritic rats |
| topic | apis dorsata honey inflammation mmp-3 osteoarthritis peanut shells extract |
| url | https://ejournal2.undip.ac.id/index.php/jbtr/article/view/7908 |
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