Impact of genetic variation on three dimensional structure and function of proteins.
The Protein Data Bank (PDB; http://wwpdb.org) was established in 1971 as the first open access digital data resource in biology with seven protein structures as its initial holdings. The global PDB archive now contains more than 126,000 experimentally determined atomic level three-dimensional (3D) s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5351996?pdf=render |
| _version_ | 1811319781573263360 |
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| author | Roshni Bhattacharya Peter W Rose Stephen K Burley Andreas Prlić |
| author_facet | Roshni Bhattacharya Peter W Rose Stephen K Burley Andreas Prlić |
| author_sort | Roshni Bhattacharya |
| collection | DOAJ |
| description | The Protein Data Bank (PDB; http://wwpdb.org) was established in 1971 as the first open access digital data resource in biology with seven protein structures as its initial holdings. The global PDB archive now contains more than 126,000 experimentally determined atomic level three-dimensional (3D) structures of biological macromolecules (proteins, DNA, RNA), all of which are freely accessible via the Internet. Knowledge of the 3D structure of the gene product can help in understanding its function and role in disease. Of particular interest in the PDB archive are proteins for which 3D structures of genetic variant proteins have been determined, thus revealing atomic-level structural differences caused by the variation at the DNA level. Herein, we present a systematic and qualitative analysis of such cases. We observe a wide range of structural and functional changes caused by single amino acid differences, including changes in enzyme activity, aggregation propensity, structural stability, binding, and dissociation, some in the context of large assemblies. Structural comparison of wild type and mutated proteins, when both are available, provide insights into atomic-level structural differences caused by the genetic variation. |
| first_indexed | 2024-04-13T12:48:32Z |
| format | Article |
| id | doaj.art-6aae8dfc7f7a461da22980ac7a9f8255 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T12:48:32Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6aae8dfc7f7a461da22980ac7a9f82552022-12-22T02:46:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01123e017135510.1371/journal.pone.0171355Impact of genetic variation on three dimensional structure and function of proteins.Roshni BhattacharyaPeter W RoseStephen K BurleyAndreas PrlićThe Protein Data Bank (PDB; http://wwpdb.org) was established in 1971 as the first open access digital data resource in biology with seven protein structures as its initial holdings. The global PDB archive now contains more than 126,000 experimentally determined atomic level three-dimensional (3D) structures of biological macromolecules (proteins, DNA, RNA), all of which are freely accessible via the Internet. Knowledge of the 3D structure of the gene product can help in understanding its function and role in disease. Of particular interest in the PDB archive are proteins for which 3D structures of genetic variant proteins have been determined, thus revealing atomic-level structural differences caused by the variation at the DNA level. Herein, we present a systematic and qualitative analysis of such cases. We observe a wide range of structural and functional changes caused by single amino acid differences, including changes in enzyme activity, aggregation propensity, structural stability, binding, and dissociation, some in the context of large assemblies. Structural comparison of wild type and mutated proteins, when both are available, provide insights into atomic-level structural differences caused by the genetic variation.http://europepmc.org/articles/PMC5351996?pdf=render |
| spellingShingle | Roshni Bhattacharya Peter W Rose Stephen K Burley Andreas Prlić Impact of genetic variation on three dimensional structure and function of proteins. PLoS ONE |
| title | Impact of genetic variation on three dimensional structure and function of proteins. |
| title_full | Impact of genetic variation on three dimensional structure and function of proteins. |
| title_fullStr | Impact of genetic variation on three dimensional structure and function of proteins. |
| title_full_unstemmed | Impact of genetic variation on three dimensional structure and function of proteins. |
| title_short | Impact of genetic variation on three dimensional structure and function of proteins. |
| title_sort | impact of genetic variation on three dimensional structure and function of proteins |
| url | http://europepmc.org/articles/PMC5351996?pdf=render |
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