Anopheles gambiae eicosanoids modulate Plasmodium berghei survival from oocyst to salivary gland invasion
Eicosanoids affect the immunity of several pathogen/insect models, but their role on the Anopheles gambiae response to Plasmodium is still unknown. Plasmodium berghei-infected mosquitoes were injected with an eicosanoid biosynthesis inhibitor, indomethacin (IN), or a substrate, arachidonic acid (AA)...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2014-08-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000500668&lng=en&tlng=en |
| _version_ | 1797718081386053632 |
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| author | Susana Ramos Ana Custódio Henrique Silveira |
| author_facet | Susana Ramos Ana Custódio Henrique Silveira |
| author_sort | Susana Ramos |
| collection | DOAJ |
| description | Eicosanoids affect the immunity of several pathogen/insect models, but their role on the Anopheles gambiae response to Plasmodium is still unknown. Plasmodium berghei-infected mosquitoes were injected with an eicosanoid biosynthesis inhibitor, indomethacin (IN), or a substrate, arachidonic acid (AA), at day 7 or day 12 post-infection (p.i.). Salivary gland invasion was evaluated by sporozoite counts at day 21 p.i. IN promoted infection upon sporozoite release from oocysts, but inhibited infection when sporozoites were still maturing within the oocysts, as observed by a reduction in the number of sporozoites reaching the salivary glands. AA treatment had the opposite effect. We show for the first time that An. gambiae can modulate parasite survival through eicosanoids by exerting an antagonistic or agonistic effect on the parasite, depending on its stage of development. |
| first_indexed | 2024-03-12T08:46:51Z |
| format | Article |
| id | doaj.art-6ab0e059670647cfa7089f4869ef1a18 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:46:51Z |
| publishDate | 2014-08-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-6ab0e059670647cfa7089f4869ef1a182023-09-02T16:26:10ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602014-08-01109566867110.1590/0074-0276140098S0074-02762014000500668Anopheles gambiae eicosanoids modulate Plasmodium berghei survival from oocyst to salivary gland invasionSusana RamosAna CustódioHenrique SilveiraEicosanoids affect the immunity of several pathogen/insect models, but their role on the Anopheles gambiae response to Plasmodium is still unknown. Plasmodium berghei-infected mosquitoes were injected with an eicosanoid biosynthesis inhibitor, indomethacin (IN), or a substrate, arachidonic acid (AA), at day 7 or day 12 post-infection (p.i.). Salivary gland invasion was evaluated by sporozoite counts at day 21 p.i. IN promoted infection upon sporozoite release from oocysts, but inhibited infection when sporozoites were still maturing within the oocysts, as observed by a reduction in the number of sporozoites reaching the salivary glands. AA treatment had the opposite effect. We show for the first time that An. gambiae can modulate parasite survival through eicosanoids by exerting an antagonistic or agonistic effect on the parasite, depending on its stage of development.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000500668&lng=en&tlng=eneicosanoidAnophelesPlasmodium |
| spellingShingle | Susana Ramos Ana Custódio Henrique Silveira Anopheles gambiae eicosanoids modulate Plasmodium berghei survival from oocyst to salivary gland invasion Memorias do Instituto Oswaldo Cruz eicosanoid Anopheles Plasmodium |
| title | Anopheles gambiae eicosanoids modulate Plasmodium berghei survival from oocyst to salivary gland invasion |
| title_full | Anopheles gambiae eicosanoids modulate Plasmodium berghei survival from oocyst to salivary gland invasion |
| title_fullStr | Anopheles gambiae eicosanoids modulate Plasmodium berghei survival from oocyst to salivary gland invasion |
| title_full_unstemmed | Anopheles gambiae eicosanoids modulate Plasmodium berghei survival from oocyst to salivary gland invasion |
| title_short | Anopheles gambiae eicosanoids modulate Plasmodium berghei survival from oocyst to salivary gland invasion |
| title_sort | anopheles gambiae eicosanoids modulate plasmodium berghei survival from oocyst to salivary gland invasion |
| topic | eicosanoid Anopheles Plasmodium |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000500668&lng=en&tlng=en |
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