![Diversity and antibacterial activity of endophytic fungi of the Amazonian medicinal plant sucuba [Himatanthus sucuuba (Spruce ex Müll. Arg.) Woodson]](/Cover/Show?author=Fernanda+Viana-Diniz&callnumber=&size=medium&title=Diversity+and+antibacterial+activity+of+endophytic+fungi+of+the+Amazonian+medicinal+plant+sucuba+%5BHimatanthus+sucuuba+%28Spruce+ex+M%C3%BCll.+Arg.%29+Woodson%5D&recordid=doaj.art-6ab281aa72d34666953c3abcf2ff48be&source=Solr&issn=0370-3908)
Diversity and antibacterial activity of endophytic fungi of the Amazonian medicinal plant sucuba [Himatanthus sucuuba (Spruce ex Müll. Arg.) Woodson]
The Amazonian medicinal plant sucuba (Himatanthus sucuuba) is widely used in ethnopharmacology and has pharmacological potential. Its endophytic fungi also have important bioactive substances of interest to the pharmaceutical industry.Despite this, there are no studies in the literature about its e...
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Academia Colombiana de Ciencias Exactas, Físicas y Naturales
2022-03-01
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| Series: | Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales |
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| Online Access: | https://raccefyn.co/index.php/raccefyn/article/view/1525 |
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| author | Fernanda Viana-Diniz Maria Francineide Ribeiro-Gomes Atilon Vasconcelos de-Araújo Leila Priscila-Peters Clarice Maia-Carvalho |
| author_facet | Fernanda Viana-Diniz Maria Francineide Ribeiro-Gomes Atilon Vasconcelos de-Araújo Leila Priscila-Peters Clarice Maia-Carvalho |
| author_sort | Fernanda Viana-Diniz |
| collection | DOAJ |
| description |
The Amazonian medicinal plant sucuba (Himatanthus sucuuba) is widely used in ethnopharmacology and has pharmacological potential. Its endophytic fungi also have important bioactive substances of interest to the pharmaceutical industry.Despite this, there are no studies in the literature about its endophytic microorganisms. In this context, this work aimed to describe endophytic fungi of H. sucuuba and evaluate the antibacterial potential of its secondary metabolites against Staphylococcus aureus, Streptococcus pneumoniae,Escherichia coli, and Klebsiella pneumoniae. We isolated leaves and stem material using the culturemedia potato-dextrose-agar (PDA) and Sabouraud-dextroseagar (SDA) with or without 10 % of plantextract and then incubated at 18°C or 28°C. The isolated fungi were analyzed for macromorphological characteristics and classified into morphospecies for micromorphological characterization and production of fungal extracts. The fungi were grouped into 259 morphospecies, 135 (52.12%) were identified at the genus level, and 16 different genera were described. Endophytic fungi were most frequently isolated from H. sucuuba leaf (341 fungi) in the SDA culture medium at 28°C. H. sucuuba showed high diversity with a Shannon index of 5.26, a Simpson’s diversity (D) index of 0.99, and an evenness index of 0.83. Of the 235 fungal extracts tested, 28 (12%) inhibited the growth of Gram-positive and Gram-negative bacteria. S. pneumoniae and E. coli were the most sensitive and K.pneumoniae the most resistant. This is the first report of studies with endophytic fungi from H.sucuuba indicating their potential for the production of antibacterial substances.
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| institution | Directory Open Access Journal |
| issn | 0370-3908 2382-4980 |
| language | English |
| last_indexed | 2024-04-13T23:23:24Z |
| publishDate | 2022-03-01 |
| publisher | Academia Colombiana de Ciencias Exactas, Físicas y Naturales |
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| series | Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales |
| spelling | doaj.art-6ab281aa72d34666953c3abcf2ff48be2022-12-22T02:25:10ZengAcademia Colombiana de Ciencias Exactas, Físicas y NaturalesRevista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales0370-39082382-49802022-03-014617810.18257/raccefyn.1525Diversity and antibacterial activity of endophytic fungi of the Amazonian medicinal plant sucuba [Himatanthus sucuuba (Spruce ex Müll. Arg.) Woodson]Fernanda Viana-Diniz0Maria Francineide Ribeiro-Gomes1Atilon Vasconcelos de-Araújo2Leila Priscila-Peters3Clarice Maia-Carvalho4Programa de Pós-graduação em Ciência, Inovação e Tecnologia para a Amazônia, Universidade Federal do Acre, Rio Branco, Acre, Brazil Programa de Pós-graduação em Ciência, Inovação e Tecnologia para a Amazônia, Universidade Federal do Acre, Rio Branco, Acre, Brazil Programa de Pós-graduação em Biodiversidade e Biotecnologia da Rede Bionorte, Universidade Federal do Acre, Rio Branco, Acre, BrazilPrograma de Pós-graduação em Ciência, Inovação e Tecnologia para a Amazônia, Universidade Federal do Acre, Rio Branco, Acre, Brazil Programa de Pós-graduação em Ciência, Inovação e Tecnologia para a Amazônia, Universidade Federal do Acre, Rio Branco, Acre, Brazil - Programa de Pós-graduação em Biodiversidade e Biotecnologia da Rede Bionorte, Universidade Federal do Acre, Rio Branco, Acre, Brazil - Centro de Ciências Biológicas e da Natureza, Universidade Federal do Acre, Rio Branco, Acre, Brazil The Amazonian medicinal plant sucuba (Himatanthus sucuuba) is widely used in ethnopharmacology and has pharmacological potential. Its endophytic fungi also have important bioactive substances of interest to the pharmaceutical industry.Despite this, there are no studies in the literature about its endophytic microorganisms. In this context, this work aimed to describe endophytic fungi of H. sucuuba and evaluate the antibacterial potential of its secondary metabolites against Staphylococcus aureus, Streptococcus pneumoniae,Escherichia coli, and Klebsiella pneumoniae. We isolated leaves and stem material using the culturemedia potato-dextrose-agar (PDA) and Sabouraud-dextroseagar (SDA) with or without 10 % of plantextract and then incubated at 18°C or 28°C. The isolated fungi were analyzed for macromorphological characteristics and classified into morphospecies for micromorphological characterization and production of fungal extracts. The fungi were grouped into 259 morphospecies, 135 (52.12%) were identified at the genus level, and 16 different genera were described. Endophytic fungi were most frequently isolated from H. sucuuba leaf (341 fungi) in the SDA culture medium at 28°C. H. sucuuba showed high diversity with a Shannon index of 5.26, a Simpson’s diversity (D) index of 0.99, and an evenness index of 0.83. Of the 235 fungal extracts tested, 28 (12%) inhibited the growth of Gram-positive and Gram-negative bacteria. S. pneumoniae and E. coli were the most sensitive and K.pneumoniae the most resistant. This is the first report of studies with endophytic fungi from H.sucuuba indicating their potential for the production of antibacterial substances. https://raccefyn.co/index.php/raccefyn/article/view/1525Antibiotics Bioprospecting SucubaEndophytic diversity |
| spellingShingle | Fernanda Viana-Diniz Maria Francineide Ribeiro-Gomes Atilon Vasconcelos de-Araújo Leila Priscila-Peters Clarice Maia-Carvalho Diversity and antibacterial activity of endophytic fungi of the Amazonian medicinal plant sucuba [Himatanthus sucuuba (Spruce ex Müll. Arg.) Woodson] Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales Antibiotics Bioprospecting Sucuba Endophytic diversity |
| title | Diversity and antibacterial activity of endophytic fungi of the Amazonian medicinal plant sucuba [Himatanthus sucuuba (Spruce ex Müll. Arg.) Woodson] |
| title_full | Diversity and antibacterial activity of endophytic fungi of the Amazonian medicinal plant sucuba [Himatanthus sucuuba (Spruce ex Müll. Arg.) Woodson] |
| title_fullStr | Diversity and antibacterial activity of endophytic fungi of the Amazonian medicinal plant sucuba [Himatanthus sucuuba (Spruce ex Müll. Arg.) Woodson] |
| title_full_unstemmed | Diversity and antibacterial activity of endophytic fungi of the Amazonian medicinal plant sucuba [Himatanthus sucuuba (Spruce ex Müll. Arg.) Woodson] |
| title_short | Diversity and antibacterial activity of endophytic fungi of the Amazonian medicinal plant sucuba [Himatanthus sucuuba (Spruce ex Müll. Arg.) Woodson] |
| title_sort | diversity and antibacterial activity of endophytic fungi of the amazonian medicinal plant sucuba himatanthus sucuuba spruce ex mull arg woodson |
| topic | Antibiotics Bioprospecting Sucuba Endophytic diversity |
| url | https://raccefyn.co/index.php/raccefyn/article/view/1525 |
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