Episomal vector reprogramming of human umbilical cord blood natural killer cells to an induced pluripotent stem cell line MUSIi013-A
Natural killer (NK) cells were isolated from human umbilical cord blood from a healthy newborn and reprogrammed by episomal vectors carrying reprograming factors L-MYC, LIN28, OCT4, SOX2, KLF4, EBNA-1, and shRNA against p53 delivered using nucleofection. The obtained MUSIi013-A human induced pluripo...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-08-01
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| Series: | Stem Cell Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506121003196 |
| _version_ | 1818912310852321280 |
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| author | Phatchanat Klaihmon Sudjit Luanpitpong Chanchao Lorthongpanich Chuti Laowtammathron Supaporn Waeteekul Papussorn Terbto Surapol Issaragrisil |
| author_facet | Phatchanat Klaihmon Sudjit Luanpitpong Chanchao Lorthongpanich Chuti Laowtammathron Supaporn Waeteekul Papussorn Terbto Surapol Issaragrisil |
| author_sort | Phatchanat Klaihmon |
| collection | DOAJ |
| description | Natural killer (NK) cells were isolated from human umbilical cord blood from a healthy newborn and reprogrammed by episomal vectors carrying reprograming factors L-MYC, LIN28, OCT4, SOX2, KLF4, EBNA-1, and shRNA against p53 delivered using nucleofection. The obtained MUSIi013-A human induced pluripotent stem cell (iPSC) line highly expressed pluripotency markers, had the capacity to differentiate into derivatives of the three germ layers, while retained a normal karyotype. This cell line may be a useful tool to study epigenic memory that may predispose hiPSCs to enhanced NK differentiation. |
| first_indexed | 2024-12-19T23:12:34Z |
| format | Article |
| id | doaj.art-6ab2f93043734c53875893df6ddf6658 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-19T23:12:34Z |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-6ab2f93043734c53875893df6ddf66582022-12-21T20:02:10ZengElsevierStem Cell Research1873-50612021-08-0155102472Episomal vector reprogramming of human umbilical cord blood natural killer cells to an induced pluripotent stem cell line MUSIi013-APhatchanat Klaihmon0Sudjit Luanpitpong1Chanchao Lorthongpanich2Chuti Laowtammathron3Supaporn Waeteekul4Papussorn Terbto5Surapol Issaragrisil6Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Siriraj Hospital, Bangkoknoi, Bangkok 10700, ThailandSiriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Siriraj Hospital, Bangkoknoi, Bangkok 10700, Thailand; Corresponding author.Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Siriraj Hospital, Bangkoknoi, Bangkok 10700, ThailandSiriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Siriraj Hospital, Bangkoknoi, Bangkok 10700, ThailandDivision of Medical Genetics, Department of Obstetrics & Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Siriraj Hospital, Bangkoknoi, Bangkok 10700, Thailand; Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandNatural killer (NK) cells were isolated from human umbilical cord blood from a healthy newborn and reprogrammed by episomal vectors carrying reprograming factors L-MYC, LIN28, OCT4, SOX2, KLF4, EBNA-1, and shRNA against p53 delivered using nucleofection. The obtained MUSIi013-A human induced pluripotent stem cell (iPSC) line highly expressed pluripotency markers, had the capacity to differentiate into derivatives of the three germ layers, while retained a normal karyotype. This cell line may be a useful tool to study epigenic memory that may predispose hiPSCs to enhanced NK differentiation.http://www.sciencedirect.com/science/article/pii/S1873506121003196Induced pluripotent stem celliPSCNatural killer cellsReprogrammingEpisomal vectors |
| spellingShingle | Phatchanat Klaihmon Sudjit Luanpitpong Chanchao Lorthongpanich Chuti Laowtammathron Supaporn Waeteekul Papussorn Terbto Surapol Issaragrisil Episomal vector reprogramming of human umbilical cord blood natural killer cells to an induced pluripotent stem cell line MUSIi013-A Stem Cell Research Induced pluripotent stem cell iPSC Natural killer cells Reprogramming Episomal vectors |
| title | Episomal vector reprogramming of human umbilical cord blood natural killer cells to an induced pluripotent stem cell line MUSIi013-A |
| title_full | Episomal vector reprogramming of human umbilical cord blood natural killer cells to an induced pluripotent stem cell line MUSIi013-A |
| title_fullStr | Episomal vector reprogramming of human umbilical cord blood natural killer cells to an induced pluripotent stem cell line MUSIi013-A |
| title_full_unstemmed | Episomal vector reprogramming of human umbilical cord blood natural killer cells to an induced pluripotent stem cell line MUSIi013-A |
| title_short | Episomal vector reprogramming of human umbilical cord blood natural killer cells to an induced pluripotent stem cell line MUSIi013-A |
| title_sort | episomal vector reprogramming of human umbilical cord blood natural killer cells to an induced pluripotent stem cell line musii013 a |
| topic | Induced pluripotent stem cell iPSC Natural killer cells Reprogramming Episomal vectors |
| url | http://www.sciencedirect.com/science/article/pii/S1873506121003196 |
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