Improving the Effect of Cancer Cells Irradiation with X-rays and High-Energy Protons Using Bimetallic Palladium-Platinum Nanoparticles with Various Nanostructures
Nano-sized radiosensitizers can be used to increase the effectiveness of radiation-based anticancer therapies. In this study, bimetallic, ~30 nm palladium-platinum nanoparticles (PdPt NPs) with different nanostructures (random nano-alloy NPs and ordered core-shell NPs) were prepared. Scanning transm...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/14/23/5899 |
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| author | Bartosz Klebowski Malgorzata Stec Joanna Depciuch Agnieszka Panek Dawid Krzempek Wiktor Komenda Adrianna Gałuszka-Bulaga Anna Pajor-Swierzy Jarek Baran Magdalena Parlinska-Wojtan |
| author_facet | Bartosz Klebowski Malgorzata Stec Joanna Depciuch Agnieszka Panek Dawid Krzempek Wiktor Komenda Adrianna Gałuszka-Bulaga Anna Pajor-Swierzy Jarek Baran Magdalena Parlinska-Wojtan |
| author_sort | Bartosz Klebowski |
| collection | DOAJ |
| description | Nano-sized radiosensitizers can be used to increase the effectiveness of radiation-based anticancer therapies. In this study, bimetallic, ~30 nm palladium-platinum nanoparticles (PdPt NPs) with different nanostructures (random nano-alloy NPs and ordered core-shell NPs) were prepared. Scanning transmission electron microscopy (STEM), selected area electron diffraction (SAED), energy-dispersive X-ray spectroscopy (EDS), zeta potential measurements, and nanoparticle tracking analysis (NTA) were used to provide the physicochemical characteristics of PdPt NPs. Then, PdPt NPs were added to the cultures of colon cancer cells and normal colon epithelium cells in individually established non-toxic concentrations and irradiated with the non-harmful dose of X-rays/protons. Cell viability before and after PdPt NPs-(non) assisted X-ray/proton irradiation was evaluated by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. Flow cytometry was used to assess cell apoptosis. The results showed that PdPt NPs significantly enhanced the effect of irradiation on cancer cells. It was noticed that nano-alloy PdPt NPs possess better radiosensitizing properties compared to PtPd core-shell NPs, and the combined effect against cancer cells was c.a. 10% stronger for X-ray than for proton irradiation. Thus, the radio-enhancing features of differently structured PdPt NPs indicate their potential application for the improvement of the effectiveness of radiation-based anticancer therapies. |
| first_indexed | 2024-03-09T17:51:23Z |
| format | Article |
| id | doaj.art-6ab7a168ae464fc3bf83510e968df8b1 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T17:51:23Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-6ab7a168ae464fc3bf83510e968df8b12023-11-24T10:40:35ZengMDPI AGCancers2072-66942022-11-011423589910.3390/cancers14235899Improving the Effect of Cancer Cells Irradiation with X-rays and High-Energy Protons Using Bimetallic Palladium-Platinum Nanoparticles with Various NanostructuresBartosz Klebowski0Malgorzata Stec1Joanna Depciuch2Agnieszka Panek3Dawid Krzempek4Wiktor Komenda5Adrianna Gałuszka-Bulaga6Anna Pajor-Swierzy7Jarek Baran8Magdalena Parlinska-Wojtan9Institute of Nuclear Physics Polish Academy of Sciences, 31-342 Krakow, PolandDepartment of Clinical Immunology, Jagiellonian University Medical College, 30-663 Krakow, PolandInstitute of Nuclear Physics Polish Academy of Sciences, 31-342 Krakow, PolandInstitute of Nuclear Physics Polish Academy of Sciences, 31-342 Krakow, PolandInstitute of Nuclear Physics Polish Academy of Sciences, 31-342 Krakow, PolandInstitute of Nuclear Physics Polish Academy of Sciences, 31-342 Krakow, PolandDepartment of Clinical Immunology, Jagiellonian University Medical College, 30-663 Krakow, PolandJerzy Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences, 30-239 Krakow, PolandDepartment of Clinical Immunology, Jagiellonian University Medical College, 30-663 Krakow, PolandInstitute of Nuclear Physics Polish Academy of Sciences, 31-342 Krakow, PolandNano-sized radiosensitizers can be used to increase the effectiveness of radiation-based anticancer therapies. In this study, bimetallic, ~30 nm palladium-platinum nanoparticles (PdPt NPs) with different nanostructures (random nano-alloy NPs and ordered core-shell NPs) were prepared. Scanning transmission electron microscopy (STEM), selected area electron diffraction (SAED), energy-dispersive X-ray spectroscopy (EDS), zeta potential measurements, and nanoparticle tracking analysis (NTA) were used to provide the physicochemical characteristics of PdPt NPs. Then, PdPt NPs were added to the cultures of colon cancer cells and normal colon epithelium cells in individually established non-toxic concentrations and irradiated with the non-harmful dose of X-rays/protons. Cell viability before and after PdPt NPs-(non) assisted X-ray/proton irradiation was evaluated by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. Flow cytometry was used to assess cell apoptosis. The results showed that PdPt NPs significantly enhanced the effect of irradiation on cancer cells. It was noticed that nano-alloy PdPt NPs possess better radiosensitizing properties compared to PtPd core-shell NPs, and the combined effect against cancer cells was c.a. 10% stronger for X-ray than for proton irradiation. Thus, the radio-enhancing features of differently structured PdPt NPs indicate their potential application for the improvement of the effectiveness of radiation-based anticancer therapies.https://www.mdpi.com/2072-6694/14/23/5899palladium nanoparticlesplatinum nanoparticlesbimetallic nanoparticlesgreen chemistrygallic acidradiosensitizers |
| spellingShingle | Bartosz Klebowski Malgorzata Stec Joanna Depciuch Agnieszka Panek Dawid Krzempek Wiktor Komenda Adrianna Gałuszka-Bulaga Anna Pajor-Swierzy Jarek Baran Magdalena Parlinska-Wojtan Improving the Effect of Cancer Cells Irradiation with X-rays and High-Energy Protons Using Bimetallic Palladium-Platinum Nanoparticles with Various Nanostructures Cancers palladium nanoparticles platinum nanoparticles bimetallic nanoparticles green chemistry gallic acid radiosensitizers |
| title | Improving the Effect of Cancer Cells Irradiation with X-rays and High-Energy Protons Using Bimetallic Palladium-Platinum Nanoparticles with Various Nanostructures |
| title_full | Improving the Effect of Cancer Cells Irradiation with X-rays and High-Energy Protons Using Bimetallic Palladium-Platinum Nanoparticles with Various Nanostructures |
| title_fullStr | Improving the Effect of Cancer Cells Irradiation with X-rays and High-Energy Protons Using Bimetallic Palladium-Platinum Nanoparticles with Various Nanostructures |
| title_full_unstemmed | Improving the Effect of Cancer Cells Irradiation with X-rays and High-Energy Protons Using Bimetallic Palladium-Platinum Nanoparticles with Various Nanostructures |
| title_short | Improving the Effect of Cancer Cells Irradiation with X-rays and High-Energy Protons Using Bimetallic Palladium-Platinum Nanoparticles with Various Nanostructures |
| title_sort | improving the effect of cancer cells irradiation with x rays and high energy protons using bimetallic palladium platinum nanoparticles with various nanostructures |
| topic | palladium nanoparticles platinum nanoparticles bimetallic nanoparticles green chemistry gallic acid radiosensitizers |
| url | https://www.mdpi.com/2072-6694/14/23/5899 |
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