Acute and Delayed Effects of Stress Eliciting Post-Traumatic Stress-Like Disorder Differentially Alters Fecal Microbiota Composition in a Male Mouse Model
The association between the shift in fecal resident microbiome and social conflicts with long-term consequences on psychological plasticity, such as the development of post-traumatic stress disorder (PTSD), is yet to be comprehended. We developed an aggressor-exposed (Agg-E) social stress (SS) mouse...
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.810815/full |
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| author | Allison Hoke Allison Hoke Nabarun Chakraborty Aarti Gautam Rasha Hammamieh Marti Jett |
| author_facet | Allison Hoke Allison Hoke Nabarun Chakraborty Aarti Gautam Rasha Hammamieh Marti Jett |
| author_sort | Allison Hoke |
| collection | DOAJ |
| description | The association between the shift in fecal resident microbiome and social conflicts with long-term consequences on psychological plasticity, such as the development of post-traumatic stress disorder (PTSD), is yet to be comprehended. We developed an aggressor-exposed (Agg-E) social stress (SS) mouse model to mimic warzone-like conflicts, where random life-threatening interactions took place between naïve intruder mice and aggressive resident mice. Gradually these Agg-E mice developed distinct characteristics simulating PTSD-like aspects, whereas the control mice not exposed to Agg-E SS demonstrated distinct phenotypes. To further investigate the role of Agg-E SS on the resident microbiome, 16S rRNA gene sequencing was assayed using fecal samples collected at pre-, during, and post-SS time points. A time agonist shift in the fecal microbial composition of Agg-E mice in contrast to its controls suggested a persistent impact of Agg-E SS on resident microbiota. At the taxonomic level, Agg-E SS caused a significant shift in the time-resolved ratios of Firmicutes and Bacteroidetes abundance. Furthermore, Agg-E SS caused diverging shifts in the relative abundances of Verrucomicrobia and Actinobacteria. An in silico estimation of genomic potential identified a potentially perturbed cluster of bioenergetic networks, which became increasingly enriched with time since the termination of Agg-E SS. Supported by a growing number of studies, our results indicated the roles of the microbiome in a wide range of phenotypes that could mimic the comorbidities of PTSD, which would be directly influenced by energy deficiency. Together, the present work suggested the fecal microbiome as a potential tool to manage long-term effects of social conflicts, including the management of PTSD. |
| first_indexed | 2024-12-10T17:12:36Z |
| format | Article |
| id | doaj.art-6abb2cb1c93d4cadada87e5d17eaae9c |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-10T17:12:36Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-6abb2cb1c93d4cadada87e5d17eaae9c2022-12-22T01:40:17ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-03-011210.3389/fcimb.2022.810815810815Acute and Delayed Effects of Stress Eliciting Post-Traumatic Stress-Like Disorder Differentially Alters Fecal Microbiota Composition in a Male Mouse ModelAllison Hoke0Allison Hoke1Nabarun Chakraborty2Aarti Gautam3Rasha Hammamieh4Marti Jett5Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN, United StatesMedical Readiness Systems Biology Branch, Center for Military Psychiatry and Neuroscience Research (CMPN), Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United StatesMedical Readiness Systems Biology Branch, Center for Military Psychiatry and Neuroscience Research (CMPN), Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United StatesMedical Readiness Systems Biology Branch, Center for Military Psychiatry and Neuroscience Research (CMPN), Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United StatesMedical Readiness Systems Biology Branch, Center for Military Psychiatry and Neuroscience Research (CMPN), Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United StatesMedical Readiness Systems Biology Branch, Center for Military Psychiatry and Neuroscience Research (CMPN), Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United StatesThe association between the shift in fecal resident microbiome and social conflicts with long-term consequences on psychological plasticity, such as the development of post-traumatic stress disorder (PTSD), is yet to be comprehended. We developed an aggressor-exposed (Agg-E) social stress (SS) mouse model to mimic warzone-like conflicts, where random life-threatening interactions took place between naïve intruder mice and aggressive resident mice. Gradually these Agg-E mice developed distinct characteristics simulating PTSD-like aspects, whereas the control mice not exposed to Agg-E SS demonstrated distinct phenotypes. To further investigate the role of Agg-E SS on the resident microbiome, 16S rRNA gene sequencing was assayed using fecal samples collected at pre-, during, and post-SS time points. A time agonist shift in the fecal microbial composition of Agg-E mice in contrast to its controls suggested a persistent impact of Agg-E SS on resident microbiota. At the taxonomic level, Agg-E SS caused a significant shift in the time-resolved ratios of Firmicutes and Bacteroidetes abundance. Furthermore, Agg-E SS caused diverging shifts in the relative abundances of Verrucomicrobia and Actinobacteria. An in silico estimation of genomic potential identified a potentially perturbed cluster of bioenergetic networks, which became increasingly enriched with time since the termination of Agg-E SS. Supported by a growing number of studies, our results indicated the roles of the microbiome in a wide range of phenotypes that could mimic the comorbidities of PTSD, which would be directly influenced by energy deficiency. Together, the present work suggested the fecal microbiome as a potential tool to manage long-term effects of social conflicts, including the management of PTSD.https://www.frontiersin.org/articles/10.3389/fcimb.2022.810815/fullPTSDC57BL/6Jstresssocial defeatmicrobiome |
| spellingShingle | Allison Hoke Allison Hoke Nabarun Chakraborty Aarti Gautam Rasha Hammamieh Marti Jett Acute and Delayed Effects of Stress Eliciting Post-Traumatic Stress-Like Disorder Differentially Alters Fecal Microbiota Composition in a Male Mouse Model Frontiers in Cellular and Infection Microbiology PTSD C57BL/6J stress social defeat microbiome |
| title | Acute and Delayed Effects of Stress Eliciting Post-Traumatic Stress-Like Disorder Differentially Alters Fecal Microbiota Composition in a Male Mouse Model |
| title_full | Acute and Delayed Effects of Stress Eliciting Post-Traumatic Stress-Like Disorder Differentially Alters Fecal Microbiota Composition in a Male Mouse Model |
| title_fullStr | Acute and Delayed Effects of Stress Eliciting Post-Traumatic Stress-Like Disorder Differentially Alters Fecal Microbiota Composition in a Male Mouse Model |
| title_full_unstemmed | Acute and Delayed Effects of Stress Eliciting Post-Traumatic Stress-Like Disorder Differentially Alters Fecal Microbiota Composition in a Male Mouse Model |
| title_short | Acute and Delayed Effects of Stress Eliciting Post-Traumatic Stress-Like Disorder Differentially Alters Fecal Microbiota Composition in a Male Mouse Model |
| title_sort | acute and delayed effects of stress eliciting post traumatic stress like disorder differentially alters fecal microbiota composition in a male mouse model |
| topic | PTSD C57BL/6J stress social defeat microbiome |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.810815/full |
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